A5036782278- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Advanced Electron Microscopy Techniques and Applications
- Near-Field Optical Microscopy
- Glaucoma and retinal disorders
- Photoacoustic and Ultrasonic Imaging
- Corneal surgery and disorders
- Retinal and Macular Surgery
- Cardiac electrophysiology and arrhythmias
- Cell Image Analysis Techniques
- Coronary Interventions and Diagnostics
- Laser Material Processing Techniques
- Force Microscopy Techniques and Applications
- Lipid Membrane Structure and Behavior
- Integrated Circuits and Semiconductor Failure Analysis
- Esophageal Cancer Research and Treatment
- Cellular transport and secretion
- Gastric Cancer Management and Outcomes
- Cardiomyopathy and Myosin Studies
- Neuroscience and Neural Engineering
- Laser Design and Applications
- Photonic and Optical Devices
- Ocular and Laser Science Research
- Cell Adhesion Molecules Research
- Intraocular Surgery and Lenses
Max Planck Institute for Multidisciplinary Sciences
2024
Max Planck Institute for Biophysical Chemistry
2008-2017
Max Planck Society
2003-2014
University of Göttingen
2012
Cornell University
2012
German Centre for Cardiovascular Research
2012
Université Paris Cité
2012
Max Planck Institute for Dynamics and Self-Organization
2012
University of Maryland, Baltimore
2012
George Mason University
2012
Superresolution imaging in sharper focus An optical microscope cannot distinguish objects separated by less than half the wavelength of light. techniques have broken this “diffraction limit” and provided exciting new insights into cell biology. Still, such hit a limit at resolution about 10 nm. Balzarotti et al. describe another way localizing single molecules called MINFLUX (see Perspective Xiao Ha). As photoactivated localization microscopy stochastic reconstruction microscopy,...
The diffuseness of the ferroelectric phase transition in ${\mathrm{PbMg}}_{1/3}$${\mathrm{Nb}}_{2/3}$${\mathrm{O}}_{3}$ is proposed to be due quenched random electric fields originating from charged compositional fluctuations. They are responsible for extreme critical slowing down, freezing into nanometric domains, and slow relaxation polarization below ${\mathit{T}}_{\mathit{c}}$\ensuremath{\sim}212 K. Barkhausen jumps during poling exclude glassiness, which was conjectured previously. At...
We present video-rate (28 frames per second) far-field optical imaging with a focal spot size of 62 nanometers in living cells. Fluorescently labeled synaptic vesicles inside the axons cultured neurons were recorded stimulated emission depletion (STED) microscopy 2.5-micrometer by 1.8-micrometer field view. By reducing cross-sectional area about factor 18 below diffraction limit (260 nanometers), STED allowed us to map and describe vesicle mobility within highly confined space boutons....
<h3>Background</h3> Recent advances in high-speed scanning technology have enabled a new generation of optical coherence tomographic (OCT) systems to perform imaging at video rate. Here, handheld OCT probe capable the anterior segment eye high frame rates is demonstrated for first time. <h3>Objective</h3> To demonstrate real-time structures. <h3>Design</h3> Survey structures normal human subjects. <h3>Setting</h3> Laboratory. <h3>Main Outcome Measures</h3> Achieving segment, satisfactory...
Utilizing single fluorescent molecules as probes, we prove the ability of a far-field microscope to attain spatial resolution down 16 nm in focal plane, corresponding about 1/50 employed wavelength. The optical bandwidth expansion by nearly an order magnitude is realized saturated depletion through stimulated emission molecular state. We demonstrate that en route scale, resolving power increases with square root saturation level, which constitutes new law regarding emerging class light...
We undertake a comprehensive study of the inverse square root dependence spatial resolution on saturation factor in stimulated emission depletion (STED) microscopy and generalize it to account for various focal patterns. used an experimental platform featuring high quality pattern which results operation close optimal optical performance. Its superior image brightness uniform effective <25 nm are evidenced by imaging both isolated self-organized convectively assembled fluorescent beads. For...
Rationale: Transverse tubules (TTs) couple electric surface signals to remote intracellular Ca 2+ release units (CRUs). Diffraction-limited imaging studies have proposed loss of TT components as disease mechanism in heart failure (HF). Objectives: Objectives were develop quantitative super-resolution strategies for live-cell membranes intact cardiomyocytes and show that structures are progressively remodeled during HF development, causing early CRU dysfunction. Methods Results: Using...
Neurotransmitter release is achieved through the fusion of synaptic vesicles with neuronal plasma membrane (exocytosis). Vesicles are then retrieved from (endocytosis). It was hypothesized more than 3 decades ago that endosomes participate in vesicle recycling, constituting a slow endocytosis pathway required especially after prolonged stimulation. This recycling model predicts newly endocytosed fuse an endosome, which sorts (organizes) molecules and buds exocytosis-competent vesicles. We...
Significance In stimulated emission depletion (STED) fluorescence microscopy, the diffraction resolution barrier is overcome by applying a doughnut-shaped light beam that transiently switches off, confining molecular to subdiffraction-sized regions around doughnut center. Unfortunately, intensities required for high exacerbate photobleaching. Our remedy, called MINFIELD, exploits fact off-switching STED does not require of crest. By recording areas or volumes in sample, exposure avoided and...
We describe a methodology for quantitative image correction in OCT which includes procedures of nonlinear axial scanning and non-telecentric scan patterns, as well novel approach refraction layered media based on Fermat's principle. The residual spatial error obtained with fan-beam hand-held probe was reduced from several hundred micrometers to near the diffraction coherence-length limits.
We report on fast beam-scanning stimulated-emission-depletion (STED) microscopy in the visible range using for resolution enhancement compact, low cost and turn-key continuous wave (CW) fiber lasers emitting at 592 nm. Spatial resolutions of 35 to 65 nm focal plane are shown various samples including fluorescent nanoparticles, immuno-stained cells with a non-exhaustive selection 5 commonly used organic markers, living expressing yellow protein Citrine. The potential straightforward...
In STED (stimulated emission depletion) nanoscopy, the resolution and signal are limited by fluorophore de-excitation efficiency photobleaching. Here, we investigated their dependence on pulse duration power of applied light for popular 750 nm wavelength. experiments with red- orange-emitting dyes, was varied from sub-picosecond range up to continuous-wave conditions, average powers 200 mW at 80 MHz repetition rate, i.e. peak 1 kW energies 2.5 nJ. We demonstrate bleaching duration, which...
STED microscopes are commonly built using separate optical paths for the excitation and beam. As a result, beams must be co-aligned can subject to mechanical drift. Here, we present single-path microscope whose aligned by design hence is insensitive The of phase plate described which selectively modulates beam but leaves unaffected. performance single-beam setup on par with previous dual-beam designs.
Abstract Up to now, all demonstrations of reversible saturable optical fluorescence transitions (RESOLFT) superresolution microscopy living cells have relied on the use reversibly switchable fluorescent proteins (RSFP) emitting in green spectral range. Here we show RESOLFT imaging with rsCherryRev1.4, a new red‐emitting RSFP enabling spatial resolution up four times higher than diffraction barrier. By co‐expressing and red RSFPs demonstrate two‐color both for single (“donut”) beam scanning...
The extension of fluorescence nanoscopy to larger numbers molecular species concurrently visualized by distinct markers is great importance for advanced biological applications. To date, up four had been distinguished in STED experiments featuring comparatively elaborate imaging schemes and optical setups, exploiting various properties the fluorophores. Here we present a simple yet versatile design multicolour below diffraction limit. A hyperspectral detection arrangement (hyperSTED)...