A5083119633- Plant Parasitism and Resistance
- Plant and animal studies
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant biochemistry and biosynthesis
- Antioxidant Activity and Oxidative Stress
- Saffron Plant Research Studies
- Plant Gene Expression Analysis
- Plant and Biological Electrophysiology Studies
- Plant Reproductive Biology
- Nematode management and characterization studies
King Abdullah University of Science and Technology
2017-2025
Active Biotech (Sweden)
2018
Abstract Carotenoid cleavage dioxygenases (CCDs) form hormones and signaling molecules. Here we show that a member of an overlooked plant CCD subfamily from rice, name Zaxinone Synthase (ZAS), can produce zaxinone, novel apocarotenoid metabolite in vitro. Loss-of-function mutants ( zas ) contain less exhibit retarded growth showed elevated levels strigolactones (SLs), hormone determines architecture, mediates mycorrhization facilitates infestation by root parasitic weeds, such as Striga spp....
In seed plants, strigolactones (SLs) regulate architecture and induce mycorrhizal symbiosis in response to environmental cues. SLs are formed by combined activity of the carotenoid cleavage dioxygenases (CCDs) 7 8 from 9-cis-β-carotene, leading carlactone that is converted cytochromes P450 (clade 711; MAX1 Arabidopsis) into various SLs. As Physcomitrella patens possesses CCD7 CCD8 homologs but lacks MAX1, we investigated if PpCCD7 together with PpCCD8 form how deletion these enzymes...
Abscisic acid (ABA) is an important carotenoid-derived phytohormone that plays essential roles in plant response to biotic and abiotic stresses as well various physiological developmental processes. In Arabidopsis, ABA biosynthesis starts with the epoxidation of zeaxanthin by DEFICIENT 1 (ABA1) enzyme, leading epoxycarotenoids; e.g., violaxanthin. The oxidative cleavage 9-cis-epoxycarotenoids, a key regulatory step catalyzed 9-CIS-EPOXYCAROTENOID DIOXYGENASE, forms xanthoxin, which converted...
Strigolactones (SLs) are a plant hormone inhibiting shoot branching/tillering and rhizospheric, chemical signal that triggers seed germination of the noxious root parasitic Striga mediates symbiosis with beneficial arbuscular mycorrhizal fungi. Identifying specific roles canonical noncanonical SLs, two SL subfamilies, is important for developing Striga-resistant cereals engineering architecture. Here, we report rice mutants lacking SLs do not show phenotypes known SL-deficient plants,...
Summary Crocins are beneficial antioxidants and potential chemotherapeutics that give raise, together with picrocrocin, to the colour taste of saffron, most expensive spice, respectively. formed from crocetin dialdehyde is produced in Crocus sativus zeaxanthin by carotenoid cleavage dioxygenase 2L (CsCCD2L), while GjCCD4a Gardenia jasminoides , another major source crocins, converted different carotenoids, including zeaxanthin, into bacterio . To establish a biotechnological platform for...
Strigolactones (SLs) regulate many developmental processes, including shoot-branching/tillering, and mediate rhizospheric interactions. SLs originate from carlactone (CL) are structurally diverse, divided into a canonical noncanonical subfamily. Rice contains two SLs, 4-deoxyorobanchol (4DO) orobanchol (Oro), which common in different plant species. The cytochrome P450 OsMAX1-900 forms 4DO CL through repeated oxygenation ring closure, while the homologous enzyme OsMAX1-1400 hydroxylates Oro....
The plant hormone strigolactones (SLs) are secreted by roots to act as rhizospheric signals. Here, we present a protocol for characterizing plant-released SLs. We first outline all necessary steps required collection, processing, and analysis of root exudates using the C18 column SL extraction, followed liquid chromatography-mass spectrometry (LC-MS) quantification. then describe image processing SeedQuant, an open-source artificial-intelligence-based software, measuring biological activity...
The enzyme DWARF27 (D27) catalyzes the reversible isomerization of all-trans- into 9-cis-β-carotene, initiating strigolactone (SL) biosynthesis. Genomes higher plants encode two D27-homologs, D27-like1 and -like2, with unknown functions. Here, we investigated enzymatic activity biological function Arabidopsis D27-like1. In vitro assays expression in Synechocystis sp. PCC6803 revealed an unreported 13-cis/15-cis/9-cis- a 9-cis/all-trans-β-carotene isomerization. Although disruption...
Abstract The plant hormones strigolactones (SLs) regulate shoot branching and mediate the communication with symbiotic mycorrhizal fungi, but also noxious root parasitic weeds, such as Striga spp. SLs derive from carlactone (CL) are divided structurally into canonical non-canonical SLs. However, questions about particular biological functions of two groups identification SL that inhibits still unanswered, hampering targeted modification pattern towards improving architecture resistance...
Abstract Carotenoid cleavage, catalyzed by CAROTENOID CLEAVAGE DIOXYGENASEs (CCDs), provides signaling molecules and precursors of plant hormones. Recently, we showed that zaxinone, a apocarotenoid metabolite formed the CCD ZAXINONE SYNTHASE (ZAS), is growth regulator required for normal rice (Oryza sativa) development. The genome encodes three OsZAS homologs, called here OsZAS1b, OsZAS1c, OsZAS2, with unknown functions. Here, investigated enzymatic activity, expression pattern, subcellular...
Vitamin A deficiency remains a severe global health issue, which creates need to biofortify crops with provitamin carotenoids (PACs). Expanding plant cell capacity for synthesis and storing of PACs outside the plastids is promising biofortification strategy that has been little explored. Here, we engineered PAC formation sequestration in cytosol Nicotiana benthamiana leaves, Arabidopsis seeds, citrus callus cells, using fungal (Neurospora crassa) carotenoid pathway consists only three...
The yield of pearl millet, a resilient cereal crop crucial for African food security, is severely impacted by the root parasitic weed Striga hermonthica, which requires host-released hormones, called strigolactones (SLs), seed germination. Herein, we identify four SLs present in Striga-susceptible line SOSAT-C88-P10 (P10) but absent resistant 29Aw (Aw). We generate chromosome-scale genome assemblies, including gapless chromosomes each line. Striga-resistant Aw lacks 0.7 Mb segment containing...
Carotenoids are essential components of the photosynthetic apparatus and precursors plant hormones, such as strigolactones (SLs). SLs involved in various aspects development stress-response processes, including establishment root shoot architecture. SL biosynthesis begins with reversible isomerization all- trans -carotene into 9- cis -β-carotene, catalysed by DWARF27 β-carotene isomerase (D27). Sequence comparisons have revealed presence D27-related proteins eukaryotes cyanobacteria lacking...
Abstract The rice (Oryza sativa L.) α/β hydrolase D14 LIKE (D14L), a paralog of the strigolactone receptor D14, is essential for establishment arbuscular mycorrhizal (AM) symbiosis and responses to karrikins, smoke-derived compounds that regulate several developmental processes. It supposed D14L yet unidentified endogenous growth regulator. Herein, we determined effects disrupting on metabolism. Our results revealed loss function altered architecture, leading significant increase in root...
Summary Carotenoid‐derived regulatory metabolites and hormones are generally known to arise through the oxidative cleavage of a single double bond in carotenoid backbone, which yields mono‐carbonyl products called apocarotenoids. However, extended conjugated system these pigments predestines them also repeated forming dialdehyde products, diapocarotenoids, have been less investigated due their instability low abundance. Recently, we reported on short diapocarotenoid anchorene as an...
Apocarotenoid glycosylation serves as a valve regulating carotenoid homeostasis in plants and may contribute to their response photo-oxidative stress.
Strigolactones (SLs) are a plant hormone regulating different processes in development and adjusting plant's architecture to nutrition availability. Moreover, SLs released by plants communicate with beneficial fungi the rhizosphere where they are, however, abused as chemical cues inducing seed germination of root parasitic weeds, e.g. Striga spp., guiding them towards host their vicinity. Based on structure, divided into canonical non-canonical SLs. In this perspective, we describe...
Oxidative cleavage of carotenoids leads to dialdehydes (diapocarotenoids, DIALs) in addition the widely known apocarotenoids. DIALs are biologically active compounds that presumably impact human health and play different roles plant development carotenoid metabolism. However, detection plants is challenging due their instability, low abundance, poor ionization efficiency mass spectrometry. Here, we developed a solid-phase extraction derivatization protocol coupled with ultrahigh performance...
Abstract The natural growth regulator zaxinone increases the levels of phytohormones strigolactone (SL) and abscisic acid in Arabidopsis ( thaliana ) via unknown mechanisms. We demonstrate that parts effects depend on SL receptor DWARF14 At D14), karrikin KARRIKIN INSENSITIVE2 KAI2), F-Box protein MORE AXILLARY BRANCHING2 MAX2) mediates signaling SLs karrikins. Binding assays co-crystallization revealed as an additional ligand D14 antagonist interrupts interaction with MAX2. Zaxinone also...
Abstract Strigolactones (SLs) regulate many developmental processes, including shoot-branching/tillering, and mediate rhizospheric interactions. SLs are structurally diverse, divided into a canonical non-canonical sub-family. To better understand the biological function of particular SLs, we generated CRISPR/Cas9 mutants disrupted in OsMAX1-1400 or OsMAX1-1900 , which encode cytochrome P450 enzymes (CYP711A clade) contributing to SL diversity. The disruption did neither affect pattern nor...