A5030981076- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Computational Drug Discovery Methods
- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- Bioactive Compounds and Antitumor Agents
- Dietary Effects on Health
- Mosquito-borne diseases and control
- HIV/AIDS Research and Interventions
- Advanced biosensing and bioanalysis techniques
- Circadian rhythm and melatonin
- Epigenetics and DNA Methylation
- interferon and immune responses
- Genetics, Aging, and Longevity in Model Organisms
- Sex work and related issues
- Hepatitis C virus research
- Influenza Virus Research Studies
- Neuroinflammation and Neurodegeneration Mechanisms
- Primate Behavior and Ecology
- Viral gastroenteritis research and epidemiology
- Immune Cell Function and Interaction
- HIV, Drug Use, Sexual Risk
University of California, San Diego
2021-2024
University of Montana
2021
San Diego State University
2019-2020
Brain microglia (MG) may serve as a human immunodeficiency virus 1 (HIV) reservoir and ignite rebound viremia following cessation of antiretroviral therapy (ART), but they have yet to be proven harbor replication-competent HIV. Here, we isolated brain myeloid cells (BrMCs) from nonhuman primates rapid autopsy people with HIV (PWH) on ART sought evidence persistent viral infection. BrMCs predominantly displayed microglial markers, in which up 99.9% the were TMEM119+ MG. Total integrated SIV...
Abstract Pathological obesity can result from genetic predisposition, obesogenic diet, and circadian rhythm disruption. Obesity compromises function of muscle, which accounts for a majority body mass. Behavioral intervention that counteract arising genetic, diet or disruption improve muscle holds untapped potential to combat the epidemic. Here we show Drosophila melanogaster (fruit fly) subject challenges exhibits metabolic disease phenotypes in skeletal muscle; sarcomere disorganization,...
We developed viral sensor and restriction factor-cytometry by time of flight (VISOR-CyTOF), which profiles 19 sensors factors (VISORs) simultaneously in single cells, applied it to 41 postmortem tissues from people with HIV. Mucosal myeloid cells are well equipped SAMHD1 capsid DNA while CD4
The worldwide COVID-19 pandemic caused by the coronavirus SARS-CoV-2 urgently demands novel direct antiviral treatments. main protease (Mpro) and papain-like (PLpro) are attractive drug targets among coronaviruses due to their essential role in processing polyproteins translated from viral RNA. In this study, we virtually screened 688 naphthoquinoidal compounds derivatives against Mpro of SARS-CoV-2. Twenty-four were selected evaluated biochemical assays using a fluorogenic substrate....
BackgroundWe examined HIV prevalence and transmission dynamics among people who inject drugs in the U.S./Mexico border region during COVID-19 pandemic.MethodsPeople aged ≥18 years from 3 groups were recruited: live San Diego (SD) engaged cross-border drug use Tijuana, Mexico (SD CBDUs), SD Tijuana (TJ) did not engage (NCBDUs). We computed at baseline bivariate incidence-density rates (IR) 18-month follow-up. Bayesian phylogenetic analysis was used to identify local clusters, estimate their...
Infections of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have triggered a global pandemic with millions deaths worldwide. Herein, synthesis functionalized Re(i) tricarbonyl complexes as inhibitors SARS-CoV-2 main protease, also referred to 3-chymotrypsin-like protease (3CLpro), is presented. The metal were found inhibit activity enzyme IC50 values in low micromolar range. Mass spectrometry revealed that formed coordinate covalent bond enzyme. Chiral separation...
One inhibitor of the main SARS-CoV-2 protease has been approved recently by FDA, yet it targets only (Mpro). Here, we discovered inhibitors containing thiuram disulfide or dithiobis-(thioformate) tested against three key proteases involved in replication, including Mpro, papain-like (PLpro), and human cathepsin L. The use covalent warheads was inspired an idea to find a better alternative than disulfiram, treatment for chronic alcoholism that is currently phase 2 clinical trials SARS-CoV-2....
The worldwide COVID-19 pandemic caused by the coronavirus SARS-CoV-2 urgently demands novel direct antiviral treatments. main protease (Mpro) and papain-like (PLpro) are attractive drug targets among coronaviruses due to their essential role in processing polyproteins translated from viral RNA. In present work, we virtually screened 688 naphthoquinoidal compounds derivatives against Mpro of SARS-CoV-2. Twenty-four were selected evaluated biochemical assays using a fluorogenic substrate....
Objective Identify system-level features in HIV migration within a host across body tissues. Evaluate heterogeneity the presence and magnitude of these hosts. Method Using DNA deep sequencing data generated multiple tissues from 8 people with HIV, we represent complex dependencies among as network model networks using family exponential random graph models (ERGMs). ERGMs allow for statistical assessment whether occur more (or less) frequently viral than might be expected by chance. The...
Objective: We sought to determine if standard influenza and pneumococcal vaccines can be used stimulate HIV reservoirs during antiretroviral therapy (ART). Design: A prospective, randomized, double-blinded, placebo-controlled, crossover trial of two clinically recommended (influenza pneumococcal). Methods: Persons with on ART ( N = 54) were enrolled in the clinical trial. Blood was collected at baseline days 2,4,7,14, 30 postimmunizations. Levels cellular RNA DNA measured by ddPCR....