A5007885328- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Advanced Fluorescence Microscopy Techniques
- Near-Field Optical Microscopy
- Plasmonic and Surface Plasmon Research
- Gold and Silver Nanoparticles Synthesis and Applications
- Advanced Electron Microscopy Techniques and Applications
- Quantum Dots Synthesis And Properties
- DNA and Nucleic Acid Chemistry
- Nanowire Synthesis and Applications
- Orbital Angular Momentum in Optics
- Cell Image Analysis Techniques
- Molecular Junctions and Nanostructures
- Graphene research and applications
Center for NanoScience
2018-2022
Ludwig-Maximilians-Universität München
2018-2022
Technische Universität Braunschweig
2014-2018
LMU Klinikum
2018
Abstract Over the last decade, two fields have dominated attention of sub-diffraction photonics research: plasmonics and fluorescence nanoscopy. Nanoscopy based on single-molecule localization offers a practical way to explore plasmonic interactions with nanometre resolution. However, this seemingly straightforward technique may retrieve false positional information. Here, we make use DNA origami both control nanometric separation between emitters gold nanoparticle, as platform for...
Abstract Graphene is considered a game‐changing material, especially for its mechanical and electrical properties. This work exploits that graphene almost transparent but quenches fluorescence in range up to ≈40 nm. as broadband unbleachable energy‐transfer acceptor without labeling, used precisely determine the height of molecules with respect graphene, visualize dynamics DNA nanostructures, orientation Förster‐type resonance energy transfer (FRET) pairs. Using origami nanopositioners,...
In recent years, DNA origami nanorulers for superresolution (SR) fluorescence microscopy have been developed from fundamental proof-of-principle experiments to commercially available test structures. The self-assembled nanostructures allow placing a defined number of fluorescent dye molecules in geometries the nanometer range. Besides unprecedented control over matter on nanoscale, robust are reproducibly obtained high yields. distances between their marks can be easily analysed yielding...
Single-molecule localization based super-resolution microscopy has revolutionized optical and routinely allows for resolving structural details down to a few nanometers. However, there exists rather large discrepancy between lateral axial accuracy, the latter typically three five times worse than former. Here, we use single-molecule metal-induced energy transfer (smMIET) localize single molecules along axis, measure their distance with an accuracy of 5 nm. smMIET relies only on fluorescence...
Abstract Resolution of emerging superresolution microscopy is commonly characterized by the width a point‐spread‐function or localization accuracy single molecules. In contrast, resolution defined as ability to separate two objects. Recently, DNA origamis have been proven valuable scaffold for self‐assembled nanorulers in microscopy. Here, we use origami overcome discrepancy localizing objects and separating resolving docking sites at distances 18, 12, 6 nm using technique PAINT(point...
Abstract Photostability is one of the crucial properties a fluorophore which strongly influences quality single molecule-based super-resolution imaging. Enhanced yellow fluorescent protein (eYFP) most widely used versions proteins in modern cell biology exhibiting fast intrinsic blinking and reversible photoactivation by UV light. Here, we developed an assay for studying photostabilization eYFP molecules with respect to demonstrated 6-fold enhanced photostability beneficial influence on...
A rectangular DNA origami structure is one of the most studied and often used motif for applications in nanotechnology. Here, we present two assays to study structural changes nanostructures reveal a reversible rolling-up induced by bivalent cations such as magnesium or calcium. First, applied one-color two-color superresolution DNA-PAINT with protruding strands along long edges rectangle. At increasing salt concentration, single line instead lines observed first indicator rolling-up....
The combination of DNA nanotechnology and single-molecule biochemistry allows the first step towards investigation structural organization a protein<italic>via</italic>SR microscopy.