A5067051002- RNA Research and Splicing
- RNA modifications and cancer
- MicroRNA in disease regulation
- Electrochemical Analysis and Applications
- Neurogenetic and Muscular Disorders Research
- RNA Interference and Gene Delivery
- Wound Healing and Treatments
- Metal complexes synthesis and properties
- Alzheimer's disease research and treatments
- Corrosion Behavior and Inhibition
- Computational Drug Discovery Methods
- Advanced biosensing and bioanalysis techniques
- Hydrogels: synthesis, properties, applications
- Genetic Syndromes and Imprinting
- RNA and protein synthesis mechanisms
- Tryptophan and brain disorders
- Circular RNAs in diseases
- Cervical Cancer and HPV Research
- Reproductive tract infections research
- Proteoglycans and glycosaminoglycans research
- Renal and related cancers
- Cancer-related molecular mechanisms research
- Biochemical effects in animals
- Extracellular vesicles in disease
- Cystic Fibrosis Research Advances
Rosalind Franklin University of Medicine and Science
2012-2024
Lewis University
2016-2024
University of Michigan
2024
Children's Healthcare of Atlanta
2023
Emory University
2023
University of California, San Diego
2012
MicroRNAs (miRNAs) are released from cells in association with proteins or microvesicles. We previously reported that malignant transformation changes the assortment of miRNAs by affecting whether a particular miRNA species is retained cell. How this selectivity occurs unclear. Here we report selectively exported miRNAs, whose release increased cells, packaged structures different those carry neutrally (n-miRNAs), not affected malignancy. By separating breast cancer cell microvesicles, find...
Canonical microRNA biogenesis requires the Microprocessor components, Drosha and DGCR8, to generate precursor-miRNA, Dicer form mature miRNA. The is not required for processing of some miRNAs, including mirtrons, in which spliceosome-excised introns are direct substrates. In this study, we examine putative human mirtrons demonstrate that although splicing-dependent, as expected, predicted miR-1225 miR-1228, produced absence splicing. Remarkably, knockout cell lines knockdown experiments...
Spinal muscular atrophy (SMA) is one of the most common inherited causes pediatric mortality. SMA caused by deletions or mutations in survival motor neuron 1 (SMN1) gene, which results SMN protein deficiency. Humans have a centromeric copy SMN2, nearly identical to SMN1. However, SMN2 cannot compensate for loss SMN1 because has single-nucleotide difference exon 7, negatively affects splicing exon. As result, mRNA produced from lacks 7. lacking 7 encodes truncated with reduced functionality....
Human papillomavirus type 16 (HPV16) has been identified as being the most common etiological agent leading to cervical cancer. Despite having a clear understanding of role HPV16 in oncogenesis, details how traffics during infection are poorly understood. determined enter via clathrin-mediated endocytosis, but subsequent steps remain unclear. There is emerging evidence that several viruses take advantage cross talk between routes endocytosis. Specifically, JCV and bovine 1 have shown cells...
The ribonuclease III enzyme Drosha has a central role in the biogenesis of microRNA (miRNA) by binding and cleaving hairpin structures primary RNA transcripts into precursor miRNAs (pre-miRNAs). Many miRNA genes are located within protein-coding host cleaved manner that is coincident with splicing introns spliceosome. close proximity pre-miRNA suggests potential for co-regulation gene expression, though this relationship not completely understood. Here, we describe cleavage-independent an...
Autosomal dominant polycystic kidney disease (ADPKD) is characterized by the growth of renal cysts that ultimately destroy function. Mutations in PKD1 and PKD2 genes cause ADPKD. Their protein products, polycystin-1 (PC1) polycystin-2 (PC2) have been proposed to form a calcium-permeable receptor-channel complex; however mechanisms which they function are almost completely unknown. Most mutations truncating loss-of-function or affect biogenesis, trafficking stability reveal very little about...
Most noncoding RNAs function properly only when folded into complex three-dimensional (3D) structures, but the experimental determination of these structures remains challenging. Understanding primary microRNA (miRNA) maturation is currently limited by a lack determined for nonprocessed forms RNA. SHAPE chemistry efficiently determines RNA secondary structural information with single-nucleotide resolution, providing constraints suitable input MC-Pipeline refinement 3D structure models. Here...
MicroRNAs (miRNAs) are regulators of gene expression, and their dysregulation is linked to cancer other diseases, making them important therapeutic targets. Several strategies for targeting modulating miRNA activity being explored. For example, steric-blocking antisense oligonucleotides (ASOs) can reduce by either blocking binding sites on specific mRNAs or base-pairing the itself prevent its interaction with target mRNAs. ASOs have been less explored as a tool elevate levels, which could...
The leading hypothesis for Alzheimer's Disease (AD) has traditionally focused on the aggregation of Amyloid-β into amyloid plaques. However, research yet to definitively prove role peptide in pathology disease. Given that all therapeutics targeting plaques have failed clinical trials, with one exception, there is a need explore alternative approaches treatment this Therefore, we examined other factors are altered during AD pathogenesis. Many patients dysregulation metal ions, such as copper...
Supramolecular nanocomposite materials have emerged as a leading interdisciplinary research area that exploits synergistic relationships at the nanoscale to enhance properties (mechanical and chemical) of next-generation biopolymeric materials. Hydrogels synthesized from natural biopolymers because their intrinsic such noncytotoxicity biodegradability well well-defined three-dimensional, noncovalent network is ideal for modification functionalization. Therefore, it critical develop...
Chronic wound treatment requires the ability to regulate healing process and mitigate propagation of bacterial infection with constant monitoring, in real-time. The need for multi-functional management materials has emerged as a significant research area promise shown incorporation antimicrobial agents within responsive polymeric elicit signals providing dynamic information about process. Traditional dressings provide barrier between outside environment wound; however, these methods are...
Alzheimer’s patients are found to have high concentrations of Amyloid-Beta peptide (A β-42 ), an overexpressed 42 amino acid sequence, along with a concentration copper ions in the brain. The A results from cleavage precursor protein (APP) and aggregates form beta sheets during post-translational modification due oxidative stress. Free metal ions, particularly Cu 2+ , initiate this stress by binding 14 th Histidine residue primary structure undergoing single electron-transfer reduction + ....
The Amyloid β peptide (Aβ-42) can be expressed through cleavage of a precursor protein (APP) during post-translational modification as result oxidative stress in the brain. Once expressed, will readily form binding interactions with free metal ions, particularly Cu 2+ , initiating series redox reactions that production Reactive Oxygen Species (ROS) and amyloid plaques within This process has been implicated major contributing factor neurodegeneration Alzheimer’s patients. An increasingly...