A5028310933- 14-3-3 protein interactions
- Galectins and Cancer Biology
- Glycosylation and Glycoproteins Research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Microbial Natural Products and Biosynthesis
- Ubiquitin and proteasome pathways
- Phagocytosis and Immune Regulation
- Protein Kinase Regulation and GTPase Signaling
- Melanoma and MAPK Pathways
- Immune cells in cancer
- Immune responses and vaccinations
- Hepatitis C virus research
- Immune Cell Function and Interaction
- Proteoglycans and glycosaminoglycans research
- Complement system in diseases
- Animal Nutrition and Physiology
- Cell Adhesion Molecules Research
- Hepatitis B Virus Studies
Immunocore (United Kingdom)
2024
MRC Protein Phosphorylation and Ubiquitylation Unit
2014-2022
University of Dundee
2014-2022
Medical Research Council
2022
Capital Medical University
2021
Beijing Ditan Hospital
2021
Abstract Motivation: The 14-3-3 family of phosphoprotein-binding proteins regulates many cellular processes by docking onto pairs phosphorylated Ser and Thr residues in a constellation intracellular targets. Therefore, there is pressing need to develop new prediction methods that use an updated set 14-3-3-binding motifs for the identification targets prioritize downstream analysis >2000 potential interactors identified high-throughput experiments. Results: Here, comprehensive from...
The dimeric 14-3-3 proteins dock onto pairs of phosphorylated Ser and Thr residues on hundreds proteins, thereby regulate many events in mammalian cells. To facilitate global analyses these interactions, we developed a web resource named ANIA: ANnotation Integrated Analysis the interactome, which integrates multiple data sets 14-3-3-binding phosphoproteins. ANIA also pinpoints candidate phosphosites using predictor algorithms, assisted by our recent discovery that human 14-3-3-interactome is...
Abstract Siglec-15 is a conserved sialic acid-binding Ig-like lectin expressed on osteoclast progenitors, which plays an important role in development and function. It also by tumor-associated macrophages some tumors, where it thought to contribute the immunosuppressive microenvironment. was shown previously that engagement of macrophage-expressed with tumor cells expressing its ligand, sialyl Tn (sTn), triggered production TGF-β. In present study, we have further investigated interaction...
C-Type lectin receptor 5A (CLEC5A) is a spleen tyrosine kinase- (Syk-) coupled pattern recognition expressed on myeloid cells and involved in the innate immune response to viral bacterial infections. Activation of CLEC5A with pathogen-derived antigens leads secretion proinflammatory mediators such as TNF-α IL-6 that may provoke systemic cytokine storm, gene polymorphisms are associated severity DV infection. In addition, was mentioned context noninfectious disorders like chronic obstructive...
: Siglec-1 is a macrophage lectin-like receptor that mediates sialic acid-dependent cellular interactions. Its upregulation on macrophages in autoimmune disease was shown previously to promote inflammation through suppressing the expansion of regulatory T cells (Tregs). Here we investigate molecular basis for binding Tregs using
Macrophages (MΦ) are highly heterogenous and versatile innate immune cells involved in homeostatic responses. Activated MΦ can exist two extreme phenotypes: pro-inflammatory (M1) anti-inflammatory (M2) MΦ. These phenotypes be recapitulated vitro by using ligands of toll-like receptors (TLRs) cytokines such as IFNγ IL-4. In recent years, human induced pluripotent stem (iPSC)-derived have gained major attention, they functionally similar to monocyte-derived receptive genome editing. this...
The protein kinases PAK4, PAK5 and PAK6 comprise a family of ohnologues. In multiple cancers including melanomas most frequently carries non-synonymous mutations; PAK4 have fewer; is often amplified. To help interpret these genomic data, initially we compared the cellular regulation sister their roles in melanoma cells. common with many ohnologue kinases, each two 14-3-3-binding phosphosites which phosphoSer99 conserved. localises to leading edge cells response phorbol ester-stimulated...
Abstract The non-polymorphic HLA-E molecule offers opportunities for new universal immunotherapeutic approaches to chronic infectious diseases. Chronic Hepatitis B virus (HBV) infection is driven in part by T cell dysfunction due elevated levels of the HBV envelope (Env) protein hepatitis surface antigen (HBsAg). Here we report characterization three genotypic variants an HLA-E-binding HBsAg peptide, Env 371-379, identified through bioinformatic predictions and verified biochemical cellular...
Two experiments were conducted to determine the effects of a phytogenic feed additive (PFA) on growth performance (exp. 1) and inflammation, oxidative stress, gut permeability, morphology 2) in nursery pigs. Dietary treatments were, basal diet [CON] + 0.015% PFA (Digestarom®, Biomin Holding GmbH). In exp. 1, pigs (n=315/trt, BW=5.79 ± 0.13 kg) allotted 21 pigs/pen for total 30 pens assigned within weight blocks dietary treatments. Pigs fed ad libitum pen BW disappearance measured d 19, 30,...
Abstract Siglec-1 is a macrophage lectin-like receptor that mediates sialic acid-dependent cellular interactions. It was shown previously to promote inflammation in autoimmune disease through suppressing the expansion of regulatory T cells (Tregs). We have investigated molecular basis for binding these using vitro -induced Tregs. strongly upregulated on activated cells, but lost under resting conditions. Glycosylation changes affect were studied by comparing and Tregs RNA-Seq, glycomics,...