Nutritional improvements in intensive aquaculture production systems is necessary for the reduction of stress, maximum utilization nutritional components, and expression genetic potential fish. The objective this study was to evaluate hemato-immunological, histological parameters gut microbiota Nile tilapia fed with microalga Schizochytrium sp. Males were distributed among eight net cages (6 m3), 105 days two diets: control (CON), without sp., supplemented (SUP), 1.2% diet. final weight,...

Abstract This work evaluated the effects of dietary supplementation A-Live (phytogenic) either individually or in combination with Aquaform (potassium diformate, acidifier) on juvenile Nile tilapia ( Oreochromis niloticus ) growth performance, innate immune parameters, gut microbiome, and resistance against Francisella noatunensis subsp. orientalis challenge. Each experimental group contained 140 fishes (34.3 ± 0.33) two 150L tanks. The design consisted five groups: a negative control;...

In the present study, effects of β-glucan administration in water on growth performance and blood status (hematological indexes, immunological biochemical parameters) were evaluated Nile tilapia fingerlings (Oreochromis niloticus) under hypoxia-induced stress. parallel, effect this prebiotic (beta-glucan) was gut microbiota diversity (16S rRNA gene) normal oxygenation condition. Three treatments implemented according to beta-glucan supplementation: 0 (control), 0.1 0.3 mg L−1. Blood...

Among the potential feed additives, β-glucans are known to positively affect growth performance, blood parameters, and intestinal microbiota of fish, even ornamental species. Therefore, present study evaluated effects dietary supplementation different Saccharomyces cerevisiae concentrations (0, 0.05, 0.1, 0.2%) in juvenile angelfish (Pterophyllum scalare) over a 42-day period. Regarding no were observed on most parameters. However, 0.2% produced higher condition factor values, indicating...

In the present study, we evaluated effects of administering Enterococcus faecium in food and/or water on hematological and immunological parameters, intestinal microbiota, resistance to bacterial diseases (streptococcosis francisellosis) growth Nile tilapia. Before vivo experiment, probiotic bacteria isolated from tilapia were selected via inhibition tests. Sequencing, annotation, assembly complete genome as well other tests performed using bioinformatics tools. Three treatments implemented:...

The objective of this experiment was to evaluate the genetic diversity within three Tambaqui broodstocks (Colossoma macropomum). Eight primers were used analyze 67 individuals collected from fish farming in municipalities: Porto Real do Colégio – Alagoas (PRC), Araujo 1 Sergipe (AR1) and 2 (AR2), Brazil. Differences frequencies 88 fragments four exclusive PRC found. High polymorphism values (from 54.38% 64.38%) Shannon´s index 0.33 0.37) observed. AMOVA showed that high variation is each...

In recent years, the genetic monitoring of broodstocks in fish farming has been greatly highlighted due to its importance management and improvement their production conservation. Current study evaluates diversity four Tambaqui (Colossoma macropomum) state Rondônia (Brazil) discusses activities towards species´s correct Nine primers were employed analyze 94 specimens from farms municipalities Ji-Paraná (JP), Ouro Preto do Oeste (OP), Presidente Médici (PM) Rolim de Moura (RM). Differences...

ABSTRACT: Brycon orbignyanus, popularly known in Brazil as piracanjuba, is a fish with great economic value but whose natural population drastically decreased number during the last years. In this context, genetic variability studies of stocks and restocking programs are fundamental for adoption conservation measures. Current analysis verifies cross-amplification heterologous primers B. orbignyanus. Fifty-two species opalinus, hilarii, insignis, Prochilodus sp., Piaractus mesopotamicus,...

The Oscar (Astronotus ocellatus) is an important Amazonian species with high production potential in fish farms and commercial value for aquarists. However, limited information on the feeding characteristics, such as frequency of daily feeding, available this captivity. Therefore, objective study was to evaluate effects growth, hematology, blood biochemical parameters, liver histology, intestinal microbiota parameters juvenile Oscars. Four treatments were tested, consisting fed at a rate one...

Brycon orbignyanus is a neotropical fish found in several South American countries. This species has high ecological importance and included the list of threatened with extinction. In this study, we report development characterisation 12 microsatellite primers B. for future genetic studies. Screening markers sample 35 individuals identified seven polymorphic loci 22 alleles, one to three alleles per locus. The information content ranged from 0.054 0.542, four showing significant deviation...

The golden mussel, Limnoperna fortunei a highly invasive species in Brazil, has generated productive, economical, and biological impacts. To evaluate genetic structure variability of L. populations present fish farms the reservoirs Canoas I (CANFF), Rosana (ROSFF), Capivara (CAPFF) (Paranapanema River, Paraná, Brazil), eight microsatellite loci were amplified. Five those resulted 38 alleles. observed heterozygosity (Ho) was lower than expected (He) all populations, with deviation from...

RESUMO O objetivo do presente estudo foi determinar a diversidade e estrutura genética de seis populações naturais Prochilodus lineatus em usinas hidrelétricas (UHE) dos rios Pardo (UHE Limoeiro - LMO), Mogi-Guaçu MOG) Tietê Promissão PRO, UHE Barra Bonita BAB, Nova Avanhandava NAV Bariri BAR). Foi encontrado um total 47 alelos, com tamanhos entre 118pb 330pb. Os resultados heterozigosidade média observada (0,490 0,625) refletiram uma alta variabilidade intrapopulacional. valores distância...

Prochilodus lineatus is a South American fish of great economic and social importance that impacted by high fishing pressure. Restocking programmes have tried to be effective in recovering natural populations; however, genetic reproductive methodologies are necessary make feasible the survival released fingerlings. The variability P. subjected semi-natural artificial reproduction was evaluated. Caudal fin samples from 25 breeders an were collected at different sex ratios (1♂:1♀ 2♂:1♀), 138...

Propolis can be used as growth enhancer due to its antimicrobial, antioxidant, and immune-stimulant properties, but effects on morphometry muscle gene expression are largely unknown. The present study evaluates the influence of propolis myostatin in Nile tilapia (Oreochromis niloticus) bred net cages. Reversed males (GIFT strain) with an initial weight 170 ± 25 g were distributed a (2 x 4) factorial scheme, two diets (DPRO, commercial diet 4% ethanol extract DCON, without propolis, control)...

Este estudo teve como objetivo avaliar o desempenho do pintado hí­­brido (Pseudoplatystoma reticulatum x Leiarius marmoratus) submetido í­Â alimentação com 32% ou 40% de proteí­­na bruta (PB) na ração, analisando-se desempenho, rendimento peixe inteiro eviscerado, filé e í­­ndice gordura visceral. Foram estocados 108 peixes nas unidades experimentais, peso inicial médio 272,12 ± 74,71 g (32% PB) 231,97 87,70 (40% PB). O perí­­odo experimental foi 182 dias. final, altura corpo, largura em...

The golden mussel (Limnoperna fortunei), a mollusc originating from Asia, has caused considerable environmental and economic damage in various Brazilian river basins reservoirs. Genetic studies that focus on characterizing its invasive potential the feasibility of control measures are required. present study identified characterized 13 new microsatellite markers (of which eight were polymorphic) L. fortunei using enriched genetic libraries. analysis performed 48 individuals three populations...

This study evaluated morphometric and ultrasound information of tilapia (O. niloticus) breeders through multivariate analysis. We applied correlation, clustering, principal component analysis to a dataset composed from 222 male female the improved GIFT strain. The body weight, objective breeding program, showed high positive correlation with most parameters. formation clusters indicated characteristics responsible for muscle composition carcass weight. Some such as weight fillet (0.98 0.94...

Although hydroelectric power plants generate most electricity in Brazil, the dams provide environmental barriers which also include interruption of fish migration. Current study, conducted prior to construction São Domingos HPP on shaft Cachoeira Branca (waterfall) Verde river, state Mato Grosso do Sul, evaluated genetic diversity two natural populations dourado (Salminus brasiliensis) located downstream (Population A - PopA) and upstream B PopB) waterfall. Eight primers were employed...

The genetic diversity of Piaractus mesopotamicus (pacu) and Leporinus elongatus (piapara) broodstocks used in restocking programs the rivers Paraná Paranapanema is analyzed. One hundred twenty specimens (two each species) from fish ponds Palotina PR Brazil Salto Grande SP were assessed. Ten primers produced 96 fragments, comprising 68 (70.83%) 94 (97.92%) polymorphic fragments for P. L. broodstocks, respectively. Differences (p < 0.05) frequency 15 27 detected species, without...

ABSTRACT: Brycon gouldingi is a species of neotropical fish socioeconomic and environmental importance in the Tocantins-Araguaia Basin. Genetic studies on this are still limited, making it difficult to evaluate population structure genetic diversity natural captive stocks. Here, we aimed transferability heterologous microsatellite primers B. gouldingi. A total 30 for eight were evaluated: hilarii, opalinus, cephalus, orbignyanus, Prochilodus lineatus, argenteus, Piaractus mesopotamicus,...

Different alleles of the human and ovine prion protein gene correlate with a varying susceptibility to transmissible spongiform encephalopathies. However, pathogenic implications specific polymorphisms in bovine (PRNP) are only poorly understood. Previous studies on PRNP investigated common European North American cattle breeds. As consequence decades intensive breeding for traits, these modern breeds represent small fraction pool. In this study, we analysed native Brazilian Caracu breed,...

Tissue growth in most fishes occurs by muscular hyperplasia and hypertrophy, which are influenced different regulatory factors, such as myostatin. The current study evaluated the influence of cultivation hapas earthen ponds on diameter white muscle fibers myostatin (MSTN-1) gene GIFT Supreme varieties tilapia. Fish both were reared for 204 days then divided into four developmental stages. White samples, corresponding to 100 per slide, collected from middle region fish each variety system,...