A5032375058- Environmental DNA in Biodiversity Studies
- Identification and Quantification in Food
- Species Distribution and Climate Change
- Microbial Community Ecology and Physiology
- Aquaculture disease management and microbiota
- Molecular Biology Techniques and Applications
- Marine animal studies overview
- Marine Toxins and Detection Methods
- Turtle Biology and Conservation
- Zoonotic diseases and public health
- CRISPR and Genetic Engineering
- Genetic diversity and population structure
- Microfluidic and Bio-sensing Technologies
- Microbial infections and disease research
- Advanced Biosensing Techniques and Applications
- Marine Bivalve and Aquaculture Studies
- Data Management and Algorithms
- Herpesvirus Infections and Treatments
- Extracellular vesicles in disease
- Viral-associated cancers and disorders
- Hemoglobin structure and function
- Physiological and biochemical adaptations
- Data Analysis with R
- Research Data Management Practices
- Fish Ecology and Management Studies
U.S. Geological Survey, Wetland and Aquatic Research Center
2016-2025
United States Geological Survey
2016-2025
TGS (United Kingdom)
2025
University of Florida
2017-2023
American Geophysical Union
2023
National Institute of Standards and Technology
2023
University of Tennessee at Knoxville
2023
Cherokee Nation
2018-2019
Medical University of South Carolina
2015
College of Charleston
2015
Abstract To inform management and conservation decisions, environmental DNA (eDNA) methods are used to detect genetic material shed into the water by imperiled invasive species. Methodological enhancements needed reduce filter clogging, PCR inhibition, false-negative detections when eDNA is at low concentrations. In first of three simple experiments, we sought ameliorate clogging from particulates organic through a scaled-up, multi-filter protocol. We combined four filters in 5 mL...
A previously undescribed isoelectric focusing technology allows cell signaling to be quantitatively assessed in <25 cells. High-resolution capillary isoforms and individual phosphorylation forms resolved, often baseline, a 400-nl capillary. Key the method is photochemical capture of resolved protein forms. Once immobilized, proteins can probed with specific antibodies flowed through Antibodies bound their targets are detected by chemiluminescence. Because chemiluminescent substrates...
A set of universal guidelines is needed to determine the limit detection (LOD) in PCR-based analyses low-concentration DNA. In particular, environmental DNA (eDNA) studies require sensitive and reliable methods detect rare cryptic species through shed genetic material samples. Current strategies for assessing limits eDNA are either too stringent or subjective, possibly resulting biased estimates species' presence. Here, a conservative LOD analysis grounded analytical chemistry proposed...
ESR Endangered Species Research Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 35:101-111 (2018) - DOI: https://doi.org/10.3354/esr00880 Theme Section: Drones in conservation research Surveys of environmental DNA (eDNA): a new approach estimate occurrence Vulnerable manatee populations Margaret E. Hunter1,*, Gaia Meigs-Friend1, Jason A. Ferrante1, Aristide Takoukam Kamla2, Robert M. Dorazio1,...
Molecular approaches are becoming more prevalent for the diagnosis of neurodegenerative diseases in human medicine and can be extended to wildlife such as chronic wasting disease other prion diseases. These have been associated with exosome-bound molecular biomarkers progression, proteins micro RNA molecules (miRNA). We tested optimized a method exosomal miRNA isolation from minimally invasive, small-volume serum samples obtained white-tailed deer (Odocoileus virginianus). confirmed exosomes...
Domoic acid toxicosis (DAT) in California sea lions (Zalophus californianus) is caused by exposure to the marine biotoxin domoic and has been linked massive stranding events mortality. Diagnosis based on clinical signs addition presence of body fluids. Chronic DAT further characterized reoccurring seizures progressing status epilepticus. chronic often slow problematic, minimally invasive tests for have focus numerous recent biomarker studies. The goal this study was retrospectively profile...
Abstract The accessibility to CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR‐associated protein) genetic tools has given rise applications beyond site‐directed genome editing for the detection of DNA and RNA. These include precise diagnostic human disease pathogens, such as SARS‐CoV‐2 Zika virus. Despite technology being rapid cost‐effective, use in surveillance causative agents wildlife diseases not been prominent. This study presents development a minimally...
Trichechid herpesvirus 1 (TrHV-1) is currently the only known in any sirenian. We hypothesized that stress may lead to recrudescence of TrHV-1 manatees, thus making a potential biomarker stress. optimized and validated real-time quantitative probe hybridization PCR (qPCR) assay was used quantify manatee peripheral blood mononuclear cells (PBMCs). Average baseline loads clinically healthy wild Florida ( Trichechus manatus latirostris) population n = 42) were 40.9 ± SD 21.2 copies/100 ng DNA;...
Cytokines have important roles in the mammalian response to viral and bacterial infections, trauma, wound healing. Because of early cytokine production after physiologic stresses, regulation messenger RNA (mRNA) transcripts can be used assess immunologic responses before changes protein production. To detect immune endangered Florida manatees ( Trichechus manatus latirostris), we developed validated a panel quantitative PCR assays measure mRNA transcription levels for cytokines interferon...
We use Tempus blood RNA tubes (Applied Biosystems) during health assessments of American moose (Alces alces spp.) as a minimally invasive means to obtain RNA. Here we describe novel protocol additionally isolate high-quality DNA from the supernatant remaining after isolation methodology. Metrics used qualify quality included measuring concentration, obtaining integrity number genomic ScreenTape assay (Agilent), and running isolated on an agarose gel. Of 23 samples analyzed, average...
Abstract Genome-wide evaluations of genetic diversity and population structure are important for informing management conservation trailing-edge populations. North American moose ( Alces alces ) declining along portions the southern edge their range due to disease, species interactions, marginal habitat, all which may be exacerbated by climate change. We employed a genotyping sequencing (GBS) approach in an effort collect baseline information on variation inhabiting species’ periphery...
First posted April 17, 2023 For additional information, contact: Director, Science Analytics and SynthesisU.S. Geological SurveyP.O. Box 25046, Mail Stop 302Denver, CO 80225 The U.S. Survey Community for Data Integration annually supports small projects focusing on data integration interdisciplinary research, innovative management, demonstration of new technologies. This report provides a summary the 14 supported in fiscal year 2019 outlines their goals, activities, accomplishments....
Although many studies have characterized catarrhine and platyrrhine primate herpesviruses, little is known about herpesviruses in prosimians. We aimed to identify characterize prosimians with proliferative lymphocytic disease. DNA was extracted from tissues of 9 gray mouse lemurs (