Microbes can establish mutualistic interactions with plants and insects. Here we track the movement of an endophytic strain Streptomyces bacteria throughout a managed strawberry ecosystem. We show that isolate found in rhizosphere on flowers protects both plant pollinating honeybees from pathogens (phytopathogenic fungus Botrytis cinerea pathogenic bacteria, respectively). The pollinators transfer among plants, move into vascular bundle rhizosphere. Our results present tripartite mutualism...

Plants in nature interact with other species, among which are mutualistic microorganisms that affect plant health. The co-existence of microbial symbionts the host contributes to fitness a natural context. In turn, composition microbiota responds environment and state host, raising possibility it can be engineered benefit plant. However, technology for engineering structure microbiome is not yet available.The loss diversity reduction population density Streptomyces globisporus SP6C4, core...

Soybean (Glycine max), a crucial global crop, experiences yearly yield reduction due to diseases such as anthracnose (Colletotrichum truncatum) and root rot (Fusarium spp.). The use of fungicides, which have traditionally been employed control these phytopathogens, is now facing challenges the emergence fungicide-resistant strains. Streptomyces bacillaris S8 strain previously known produce valinomycin t through nonribosomal peptide synthetase (NRPS) pathway. objective this study was evaluate...

Streptomyces griseus S4-7 is representative of strains responsible for the specific soil suppressiveness Fusarium wilt strawberry caused by oxysporum f. sp. fragariae. Members genus secrete diverse secondary metabolites including lantipeptides, heat-stable lanthionine-containing compounds that can exhibit antibiotic activity. In this study, a class II lantipeptide provisionally named grisin, previously unknown biological function, was shown to inhibit F. oxysporum. The inhibitory activity...

Plant immune responses can be triggered by chemicals, microbes, pathogens, insects, or abiotic stresses. In particular, induced systemic resistance (ISR) refers to the activation of system due a plant's interaction with beneficial microorganisms. The phenolic compound, 2,4-diacetylphloroglucinol (DAPG), which is produced Pseudomonas spp., acts as an ISR elicitor, yet DAPG's mechanism in remains unclear. this study, transgenic Arabidopsis thaliana plants overexpressing DAPG hydrolase gene...

Kiwifruit (Actinidia spp.) is an economically important crop and a bacterial canker disease, caused by Pseudomonas syringae pv. actinidiae (Psa), the most destructive disease in kiwifruit production. Therefore, prevent control of critical issue industry worldwide. Unfortunately, there no reliable methods have been developed. Recently, interest using microbial agents growing. However, microbiota their roles mainly remaining unknown. In this study, we secured libraries from ecospheres...

Endophyte bacteria, which colonize plants including roots, stem, flower, and fruit, it can derive their nutrients from the host, are recognized for mutualistic relationship with host plant. They play a critical role in promoting growth modulating abiotic stress. Carbon nitrogen have significant impact on bacterial population secondary metabolite production, highly specific various categories such as regulation, anti-compounds production. Application of L-glutamic acid significantly enhance...

Strawberry bacterial angular leaf spot (ALS) disease, caused by Xanthomonas fragariae has become increasingly problematic in the strawberry agro-industry. ALS causes small water-soaked lesions to develop on abaxial surface. Studies reported optimum temperature conditions for X. are 20°C and pathogen suffers mortality above 32°C. However, at nursery stage, disease symptoms have been observed under high conditions. In present study, results showed transmission was via infected maternal plants,...

Ginseng is a popular medicinal herb with established therapeutic effects such as cardiovascular disease prevention, anticancer effects, and anti-inflammatory effects. However, the slow growth of ginseng due to soilborne pathogens has been challenge for establishing new plantations. In this study, we investigated root rot associated microbiota in monoculture model system. Our results showed that collapse early community inhibiting was observed before became severe, nitrogen fixation necessary...

Fire blight disease, caused by the bacterial pathogen Erwinia amylovora, has been a significant concern for over 50 countries worldwide. The efficacy of chemical pesticides currently available disease control is limited. To address this issue, research being conducted to explore environmentally friendly methods, particularly biological using beneficial microorganisms. However, there limited on apple microbiota community and minimal fungal communities that may exhibit reliable performance in...

Flowers of kiwifruit are morphologically hermaphroditic and survivable binucleate pollen is produced by the male flowers. In this study, we investigated microbial diversity in pollens analyzing amplicon sequences 16S rRNA. Four samples were collected: 'NZ' was imported from New Zealand, 'CN' China year 2014, respectively. 'KR13' 'KR14' collected 2013' 2014' South Korea. Most identified bacterial phyla four different Proteobacteria, Actinobacteria Firmicutes. However, domestic showed aspects...

Soil is the major source of plant-associated microbes. Several fungal and bacterial species live within plant tissues. Actinomycetes are well known for producing a variety antibiotics, they contribute to improving health. In our previous report, Streptomyces globisporus SP6C4 colonized tissues was able move other from initially ones. This strain has excellent antifungal antibacterial activities provides suppressive effect upon various diseases. Here, we report genome-wide analysis antibiotic...

Ginsengs, widely acknowledged for their health-promoting properties, are predominantly grown roots, necessitating an extended cultivation period of a minimum 4 to 6 years maturation. The prolonged growth duration in specific location makes ginseng plants susceptible soil-borne ailments, such as root rot, leading significant detrimental effects. Focusing on the crucial role plant microbial community maintaining health, study reveals that repeated and continuous leads collapse initial...

The phenomenon in which the damage of plant diseases is suppressed by continuous cropping defined as "suppressiveness" and development suppressive soils key beneficial microorganisms have been identified through various previous studies. However, no studies conducted on microbial communities related to disease occurrence before initial crop monoculture.

딸기에는 여러 가지 병이 발생하여 경제적으로 막대한 손실을 유발하며 많은 양의 농약이 사용되고 있는 실정이다. 최근 유기농산물과 식품안전성에 관한 소비자의 관심이 증가함에 따라 화학농약 대체제에 의한 병해 방제법 개발이 다각적으로 시도되고 있다. 본 연구에서는 광범위 소독제로 사용하고 NaDCC의 딸기 주요 병원균(딸기 시들음병, 탄저병, 역병 그리고 세균성모무늬병)에 대한 항균활성 및 방제효과를 검증하고, 친환경제제로의 가능성을 확인하고자 수행되었다. NaDCC는 150~300 ppm 농도로 처리했을 때 병원진균의 균사생장을 효과적으로 억제하였고, 포자 발아율도 68% 이상 경감시키는 것으로 조사되었다. 세균성모무늬 병원균에 대하여서도 우수한 나타내었는데, 포장조건에서 세균성 모무늬병의 발생을 50% 감소시키는 나타났다. 이상의 결과를 토대로 NADCC는 병 방제용 방제제 후보물질로 사용될 수 있을 생각되었다. Various diseases occur in fruits,...

Fire blight disease, caused by Erwinia amylovora, could damage rosaceous plants such as apples, pears, and raspberries. In this study, we designed to understand how E. amylovora affected other bacterial communities on apple rhizosphere; twig fruit endosphere; leaf, episphere. Limited studies the understanding of microbial community apples changes structure occurrence fire disease were conducted. As result these experiments, infected trees had low species richness operational taxonomic unit...

Severe bark decay disease was observed on oak logs at a shiitake cultivation farm in Geochang-gun, Gyeongnam province. The symptoms were fruiting bodies that had developed the top and side surface of logs. As result, came off easily exposing sapwood. Slime mold specimens collected from showed developing comprising stalks, hypothallus, capillitium, columella, causal agent identified as Stemonitis splendens basis morphological characteristics. To our knowledge, this is first report causing...

ABSTRACT Streptomyces is a widely studied bacterial genus, particularly with regard to secondary metabolites and antibiotics production. griseus S4–7 was isolated from strawberry Fusarium wilt disease suppressive soil, its biological control ability has been well established. However, the antifungal mechanism of strain not yet fully understood at molecular biochemical level. Therefore, in this study we created random mutant library for Tn5 transposon element investigate traits on genome-wide...

Strawberry Fusarium wilt disease, caused by oxysporum f. sp. fragariae, is the most devastating disease in strawberry production. The pathogen produces chlamydospores which tolerate against harsh environment, fungicide and survive for decades soil. Development of detection quantification techniques are regarded significantly many soilborne pathogens to prevent damage from diseases. In this study, we improved specific-quantitative primers F. fragariae reveal correlation between density...

The plant microbiota plays a crucial role in promoting health by facilitating the nutrient acquisition, abiotic stress tolerance, biotic resilience, and host immune regulation. Despite decades of research efforts, precise relationship function between plants microorganisms remain unclear. Kiwifruit (Actinidia spp.) is widely cultivated horticultural crop known for its high vitamin C, potassium, phytochemical content. In this study, we investigated microbial communities kiwifruit across...