Patients with oral squamous cell carcinoma currently experience a five-year survival rate of approximately 60% conventional surgical, chemotherapy and radiotherapy treatments. Magnetic hyperthermia offers an alternative treatment method by utilising the heating properties magnetic nanoparticles to produce thermal ablation tumour site when exposed alternating field. In this study, we investigate in vitro if targeted potential for carcinoma.Magnetic iron oxide nanoparticles, biocompatible...

Immobilized biomineralizing protein Mms6 templates the formation of uniform magnetite nanoparticles in situ when selectively patterned onto a surface. Magnetic force microscopy shows that stable particles maintain their magnetic orientation at room temperature, and may be exchange coupled. This precision-mixed biomimetic/soft-lithography methodology offers great potential for future nanodevice fabrication. Detailed facts importance to specialist readers are published as "Supporting...

Significance Magnetotactic bacteria produce morphologically precise magnetite nanoparticles within organelles termed “magnetosomes.” Biomineralization proteins tightly regulate crystallization of these nanoparticles. A master protein regulator particle morphology in vivo, magnetosome membrane specific F (MmsF), has recently been discovered. In this study, we purified MmsF and two homologous from Magnetospirillum magneticum strain AMB-1. imposes strict control over the formation when added to...

Abstract Formation of magnetite nanocrystals by magnetotactic bacteria is controlled specific proteins which regulate the particles’ nucleation and growth. One such protein Mms6. This small, amphiphilic can self‐assemble bind ferric ions to aid in formation. To understand role Mms6 during vitro iron oxide precipitation we have performed situ pH titrations. We find has little effect salt precipitation, but exerts greatest influence incorporation ferrous conversion this mixed‐valence minerals,...

While biomimetic research is becoming increasingly popular the term being used for a broader range of and it more difficult researchers to understand define. In this opinion article we discuss how overlaps with differs from complementary fields biotechnology, biokleptic bioinspired as attempt describe each area definitions, examples discussion. What makes biomimetic, or put under scrutiny ask: can different components, parts processes an experiment be categorised separately? difference...

Biopanning was used to generate novel artificial binding proteins which are able control the formation of synthetic cubic nanoparticles magnetite.

Using a surface-based mimic of magnetosome interior, the biomineralisation protein Mms6 was found to be more effective nucleator than binder magnetite nanoparticles, and performs better its C-terminal region alone.

A cysteine-mutated biomineralisation protein (Mms6) patterned onto gold biotemplates magnetic nanoparticle arrays of magnetite and higher coercivity cobalt-doped magnetite. This demonstrates an adaptable, green approach for the future nanofabrication.

Nanotechnology demands the synthesis of highly precise, functional materials, tailored for specific applications. One such example is bit patterned media. These high-density magnetic data-storage materials require and uniform nanoparticles (MNPs) to be over large areas (cm2 range) in exact nanoscale arrays. However, realisation nanotechnology applications depends upon reproducible fabrication methods that are both precise environmentally-friendly, cost-effective scale-up. A potentially ideal...

Abstract Green synthesis of precise inorganic nanomaterials is a major challenge. Magnetotactic bacteria biomineralise magnetite nanoparticles (MNPs) within membrane vesicles (magnetosomes), which are embedded with dedicated proteins that control nanocrystal formation. Some such used in vitro to MNP formation green synthesis; however, these self-aggregate, making their production and use challenging difficult scale. Here, we provide an alternative solution by displaying active loops from...

In this study, we describe a novel high throughput, micro-macro approach for the identification and efficient design of biopolymer stabilized soil systems. At "microscopic" scale, propose rapid Membrane Enabled Bio-Mineral Affinity Screening (MEBAS) supported by Mineral Binding Characterization (MBC) (TGA, ATR-FTIR ζ Potential), while at "macroscopic" micro scale results are confirmed Geotechnical Verification (GV) through unconfined compression testing. We illustrate methodology using an...

Sporulation in Bacillus subtilis begins with an asymmetric cell division producing two genetically identical cells different fates. SpoIIE is a membrane protein that localizes to the polar sites where it causes FtsZ relocate from mid-cell form Z-rings. Following septation, establishes compartment-specific gene expression smaller forespore by dephosphorylating anti-sigma factor antagonist SpoIIAA, leading release of RNA polymerase sigma σ(F) inhibitory complex SpoIIAB. therefore couples...

Many Modern Technologies, such as High Density Data Storage, Require Monodispersed Magnetic Nanoparticles (MNPs), which Have a Consistent Behavior, Specifically Immobilized onto Patterned Surface. Current Methods for Synthesizing Uniform Mnps Temperatures and Harsh Chemicals, Is Not Environmentally Friendly. Also, the Particles Are Expensive to Make Pattern Using Conventional Lithography Methods. Bacteria Able Synthesize in Vivo Biomineralization Proteins inside Magnetosome Vesicles Control...

Magnetite nanoparticles’ applicability is growing extensively. However, simple, environmentally-friendly, tunable synthesis of monodispersed iron-oxide nanoparticles challenging. Continuous flow microfluidic promising; however, the microscale results in small yields and clogging. Here we present two simple macrofluidics devices (cast machined) for precision magnetite nanoparticle utilizing formation at interface by diffusion between laminar flows, removing aforementioned issues. Ferric to...

The biomineralization protein Mms6 has been shown to be a major player in the formation of magnetic nanoparticles both within magnetosomes magnetotactic bacteria and as an additive synthetic magnetite precipitation assays. Previous studies have highlighted ferric iron binding capability this activity is thought crucial its mineralizing properties. To understand how binds ions we prepared series single amino acid substitutions C-terminal region used assay probe site at level individual...

Magnetic magnetite nanoparticles (MNP) are heralded as model vehicles for nanomedicine, particularly cancer therapeutics. However, there many methods of synthesizing different sized and coated MNP, which may affect their performance nanomedicines. Magnetosomes naturally occurring, lipid-coated MNP that exhibit exceptional hyperthermic heating, but properties, cell uptake toxicity have yet to be compared other MNP. can mimicked by coating in either amphiphilic oleic acid or silica. In this...

SpoIIE is a dual function protein that plays important roles during sporulation in Bacillus subtilis. It binds to the tubulin-like FtsZ causing cell division septum relocate from mid-cell pole, and it dephosphorylates SpoIIAA phosphate leading establishment of differential gene expression two compartments following asymmetric septation. Its 872 residue polypeptide contains multiple-membrane spanning sequence at N-terminus PP2C phosphatase domain C-terminus. The central segment unlike domains...

Protein engineering is an attractive approach for the self-assembly of nanometer-scale architectures a range potential nanotechnologies. Using versatile chemistry provided by protein folding and assembly, coupled with amino acid side-chain functionality, allows construction precise molecular "protein origami" hierarchical patterned structures nanoapplications such as stand-alone enzymatic pathways machines. The Staphyloccocus aureus surface SasG rigid, rod-like structure shown to have high...

A response to the comments raised by Drack et al. in their reply discussion of biomimicry and related fields set out our opinions paper Rawlings Here we appreciate common views share welcome clarity this gives, discuss question some criticisms original piece clarify opinion area.

A biomineralization protein from magnetotactic bacteria (Mms6) used to control both the formation and location of magnetic nanoparticles in a patterned array. Mms6 immobilized on surface templates high-quality, crystalline magnetite particles with narrow grainsize distribution consistent behavior under relatively mild conditions. These are ferrimagnetic may be exchange-coupled surface, forming multiparticle attraction/repulsion zones. The nanomaterials arrays surfaces using various specific...