Invasive Candida infection among nonneutropenic, critically ill adults is a clinical problem that has received increasing attention in recent years. Poor performance of extant diagnostic modalities promoted risk-based, preemptive prescribing view the poor outcomes associated with inadequate or delayed antifungal therapy; this risks unnecessary overtreatment. A rapid, reliable test could have substantial impact on therapeutic practice patient population.Three TaqMan-based real-time polymerase...

Acinetobacter spp. has gained fame from their ability to resist difficult conditions and constant development of antimicrobial resistance. This study aimed investigate the prevalence, susceptibility testing, OXA carbapenemase-encoding genes, RAPD-genotyping multidrug resistant baumannii incriminated in hidden community-acquired infections Egypt. The testing was assessed phenotypically using Kirby–Bauer disk diffusion method. Also, Modified-Hodge test (MHT) carried out detect carbapenemases...

Background In this study, we aimed to evaluate the knowledge and awareness about cervical cancer, human papillomavirus (HPV) its vaccine among women living in western region of Saudi Arabia. Methodology Using a cross-sectional online survey, study assesses level HPV risk factors cancer The design questionnaire is based on several earlier studies different populations. Results total completed responses include sample 624 that was subjected statistical analysis; analysis showed only 34.6% were...

The limitations of classical diagnostic methods for invasive Candida infections have led to the development molecular techniques such as real-time PCR improve diagnosis. However, detection low titres DNA in blood from patients with candidaemia requires use extraction that efficiently lyse yeast cells and recover small amounts suitable amplification. In this study, a Candida-specific assay was used detect albicans inoculated whole specimens extracted using seven different protocols. yield...

To determine the frequency of primary cytomegalovirus (CMV) infection in pregnant Egyptian women using CMV IgG avidity testing.A cross-sectional study was conducted at Suez Canal University Hospital, Ismailia, Egypt. A total 546 women, presenting for routine antenatal screening, were tested and IgM a commercially available enzyme-linked immunosorbent assay (ELISA). Sera from IgM-positive by assay.All seropositive anti-CMV IgG. Of 40 (7.3%) positive or equivocal antibodies. All sera...

The emergence and rapid spread of antibiotic-resistant Klebsiella pneumoniae isolates harbouring the bla KPC gene that encodes for carbapenemase production have complicated management patient infections.This study in a tertiary care hospital Egypt used real-time PCR assay to test ertapenem-nonsusceptible K. presence compared results with modified Hodge test.Antibiotic sensitivity was performed by standard methods, interpreted following both old CLSI breakpoints (M100-S19) carbapenems revised...

In view of both the delay in obtaining identification by conventional methods following blood-culture positivity patients with candidaemia and close relationship between species fluconazole (FLC) susceptibility, early speciation positive blood cultures has potential to influence therapeutic decisions. The aim was develop a rapid test differentiate FLC-resistant from FLC-sensitive Candida species. Three TaqMan-based real-time PCR assays were developed identify up six directly BacT/Alert...

In contrast to the multitude of studies on fungal PCR assay methods, little work has been reported evaluating Candida performance when using whole blood compared with serum in candidaemic patients. Here, a comparison whole-blood and specimens set real-time species assays is described. Specimens were collected prospectively from non-neutropenic adults who recruited diagnostic clinical trial, primary purpose which was verify serum; all, 104 participants also had submitted for analysis addition...

Multi-drug resistant (MDR) strains of Acinetobacter baumannii are responsible for an increasing number opportunistic infections in hospitals.This study determined the prevalence MDR A. isolates from intensive care units a large tertiary-care hospital Ismailia, Egypt, and occurrence different beta-lactamases these isolates.Biotyping antimicrobial susceptibility profile was done isolated strains.Respiratory, urine, burn wound blood specimens were collected 350 patients admitted to units; 10...

T regulatory cells (Treg) play an important role in the maintenance of immune cell homeostasis, as it has been reported that CD4+CD25+ suppress auto-reactive responses autoimmune diseases such systemic lupus erythematosus (SLE). The clinical significance recently identified population CD4+CD25-Foxp3+ and whether they are associated with particular organ involvement is still not clear. So, aim our study was to evaluate presence SLE patients comparison healthy controls determine their...

This study examined the T helper (Th) 1/Th17-related cytokines, interferon (IFN)-γ and interleukin (IL)-17 in serum of biopsy-proven chronic hepatitis C patients before after IFN ribavirin therapy to address whether or not viral clearance is related Th1/Th17 cytokines.The levels IFN-γ IL-17 were assayed by ELISA on 26 with hepatitic virus (HCV) infection start 3 months treatment pegylated IFN-α plus compared sera from 15 normal control subjects.IFN-γ are higher than controls these elevated...

Chronic hepatitis C virus (HCV) infection combined with occult B (HBV) has been associated increased risk of hepatitis, cirrhosis and hepatocellular carcinoma.This study aimed to determine the prevalence HBV among Egyptian chronic HCV patients, genotype occurrence surface gene mutations impact co-infection on early response treatment.The enrolled 162 patients from Ismailia Fever Hospital, Egypt, who were antigennegative.All given clinical assessment biochemical, histological virological...

Natural killer cells can be divided into five subpopulations based on the relative expression of CD16 and CD56 markers. The majority natural are CD56(dim), which considered to main cytotoxic effectors. A minority CD56(bright), function as an important source immune-regulatory cytokines. Shifts these subsets have been reported in patients with chronic hepatitis C virus infection. We sought investigate shift among Egyptian HCV analyze influence interferon therapy this shift. applied a flow...

This study evaluated the seroprevalence of hepatitis E virus among blood donors attending transfusion Center Suez Canal University Hospital from March to September, 2010. Four hundred eighty eight (488) subjects which consisted 137 Anti- Hepatitis C positive donors, 35 B surface antigens and 316 who were negative antigen, HIV included in this study. Anti-hepatitis (IgG IgM) was detected 17.7, 28.57, 26.28% for antigen (HBsAg)  virus, (HBsAg) respectively. No significant (P > 0.05)...

We evaluated the utility of 2 methods for detection methicillin-resistant Staphylococcus aureus (MRSA) directly from signal-positive blood culture bottles: loop-mediated isothermal amplification (LAMP) assay, and direct cefoxitin disk diffusion (DCDD) test using a 30 μg disk.In parallel, standard microbiological identification oxacillin susceptibility testing with MecA PCR was performed.Of 60 cultures positive Gram-positive cocci in clusters, LAMP (via FemA genes) showed 100% sensitivity...

Background: Acute pharyngitis is one of the most common illnesses. Most acute cases are caused by viral infections. To enhance appropriate prescribing antibiotics a number clinical prediction rules have been developed to distinguish streptococcal from other causes Aim: assess appropriateness antibiotic prescribed and diagnostic accuracy McIsaac modified Centor score for predicting group A beta hemolytic pharyngitis. Patients Methods: cross-sectional study; was conducted in Port Said fever...

Colistin interacts with the bacterial outer membrane by removing divalent cations from negativelycharged phosphate groups of Lipid A lipopolysaccharide which leads to cell lysis [2].

Background: Most Staphylococcus epidermidis -related infections are acquired in hospitals and associated with the use of medical devices. Objectives: The aim this study was to detect biofilm producing S. from patients catheter-related bloodstream (CRBSI) determine their antibiotic resistance pattern. Methodology: Blood catheter tip specimens were collected 150 CRBSI. Isolated strains tested for production by modified tissue culture plate (MTCP) method, Congo red agar (CRA) icaA icaD genes...

Natural killer cells can be divided into five subpopulations based on the relative expression of CD16 and CD56 markers. The majority natural are CD56dim, which considered to main cytotoxic effectors. A minority CD56bright, function as an important source immune-regulatory cytokines. Shifts these subsets have been reported in patients with chronic hepatitis C virus infection. We sought investigate shift among Egyptian HCV analyze influence interferon therapy this shift. applied a flow...