A5042614204- Voice and Speech Disorders
- Tracheal and airway disorders
- Dysphagia Assessment and Management
- Cleft Lip and Palate Research
- Phonetics and Phonology Research
- Speech Recognition and Synthesis
- Tissue Engineering and Regenerative Medicine
- Advanced Proteomics Techniques and Applications
- Respiratory and Cough-Related Research
- RNA and protein synthesis mechanisms
- Mass Spectrometry Techniques and Applications
- Glycosylation and Glycoproteins Research
- Electrospun Nanofibers in Biomedical Applications
- Head and Neck Cancer Studies
- Hearing, Cochlea, Tinnitus, Genetics
- Music and Audio Processing
- Salivary Gland Tumors Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Machine Learning in Bioinformatics
- Wound Healing and Treatments
- Biochemical Analysis and Sensing Techniques
- Mesenchymal stem cell research
- Molecular Biology Techniques and Applications
- Peptidase Inhibition and Analysis
- Wnt/β-catenin signaling in development and cancer
University of Wisconsin–Madison
2014-2023
Madison Group (United States)
2004-2021
Google (United States)
2006-2009
UW Health University Hospital
2007
Single-cell (SC) analysis provides unique insight into individual cell dynamics and cell-to-cell heterogeneity. Here, we utilize trapped ion mobility separation coupled with dual-polarity ionization mass spectrometry imaging (MSI) to enable high-throughput in situ profiling of the SC lipidome. Multimodal imaging, which dual-polarity-mode MSI is used perform serial data acquisition runs on cells, significantly enhanced lipidome coverage. High-spatial resolution SC-MSI identifies both inter-...
Abstract Objective The objective was to assess the histological and viscoelastic shear tissue properties of scarred vocal fold lamina propria at 2 6 months postoperatively in a canine model. Study Design Experimental, nonrandomized prospective study. Methods Six larynges were injured using stripping procedure. At postoperatively, analyses control samples completed for collagen, procollagen, elastin, hyaluronic acid. Results In canines killed months, contained increased procollagen decreased...
Abstract Protein turnover is critical to cellular physiology as well the growth and maintenance of tissues. The unique synthesis degradation rates each protein help define tissue phenotype, knowledge tissue- protein-specific half-lives directly relevant protein-related drug development administration medical therapies. Using stable isotope labeling mass spectrometry, we determine in vivo thousands proteins—including those extracellular matrix—in a set biologically important mouse We...
Vocal fold scarring disrupts the layer structure of vocal lamina propria that is essential for optimal mucosal vibration. Prevention remains challenging. Hepatocyte growth factor (HGF) has strong antifibrotic activity. The authors' previous studies have found HGF stimulates hyaluronic acid production and suppresses collagen from fibroblasts, suggesting therapeutic potential in prevention scarring. present study aimed to demonstrate effects on an vivo rabbit model.Animal experiment.The mucosa...
Summary Transforming growth factor (TGF)-β1 and TGF-β3 have been reported to exert differential effects on wound healing, possibly even account for tissue-specific differences in scar formation. Scarring is particularly detrimental the vocal fold mucosa (VFM), where destruction of native extracellular matrix causes irreparable biomechanical changes voice impairment. Here, a series vitro vivo experiments, we identified TGF-β1 transcription immunolocalization various cell subpopulations naïve...
Glycosylation is a major protein post-translational modification whose dysregulation has been associated with many diseases. Herein, an on-tissue chemical derivatization strategy based on positively charged hydrazine reagent (Girard's P) coupled matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was developed for analysis of N-glycans from FFPE treated tissue sections. The performance the proposed approach evaluated by monosaccharides, oligosaccharides,...
Vocal fold scarring remains a therapeutic challenge. Hepatocyte growth factor (HGF) has strong antifibrotic activity and proved to have potential in restoration of scar tissues such as liver cirrhosis lung fibrosis. The present study aimed clarify the effects HGF injection into scarred vocal folds canine model. Canine were stripped unilaterally treated with intracordal saline solution (sham group), (HGF or cultured autologous normal fibroblasts (Fb/HGF group) 1 month after injury. larynges...
Abstract Objectives/Hypothesis: The purpose of this study was to compare the clinical effectiveness type I thyroplasty, injection laryngoplasty, and graft implantation for treatment vocal fold scar pathologic sulcus vocalis. Study Design: Prospective, multi‐arm, quasi‐experimental research design. Methods: Twenty‐eight patients with newly diagnosed and/or vocalis were assigned one three modalities: thyroplasty (n = 9), laryngoplasty ( n 10). Psychosocial, auditory‐perceptual, acoustic,...
Primary human vocal fold mucosal cells recapitulate native physiologic function, offering voice restoration to patients with advanced laryngeal disease.
A structural lipidomics approach employs peracetic acid-induced epoxidation coupled with mass spectrometry for pinpointing CC bonds in unsaturated fatty acids, enabling both quantification and imaging of FA isomers from biological samples.
The objective was to measure the level of hyaluronan during first 15 days after vocal fold biopsy in a rabbit model.Experimental, nonrandomized prospective study.Twenty-eight rabbits underwent unilateral biopsy. contralateral preserved as control sample. On 3, 5, 10, and biopsy, levels injured normal folds were measured immunohistologically with an ELISA assay.Hyaluronan lowest on day 3 highest 5 injury. Statistical analysis revealed that demonstrated significantly lower than 15. greater...
Purpose To develop and evaluate a rat excised larynx model for the measurement of acoustic, aerodynamic, vocal fold vibratory changes resulting from scar. Method Twenty-four 4-month-old male Sprague–Dawley rats were assigned to 1 4 experimental groups: chronic scar, scar treated with 100-ng basic fibroblast growth factor (bFGF), saline (sham treatment), unscarred untreated control. Following tissue harvest, histological immunohistochemical data collected confirm extracellular matrix...
The vocal fold mucosa plays an important role in voice production. Its cellular composition and density frequently change under various pathological conditions, often contributing to altered extracellular matrix production, tissue viscoelasticity, quality. In this study, changes the rat following a unilateral stripping injury were investigated analyzed semi-quantitatively. Distinctive sequential morphology, composition, observed post-injury. Cellular recruitment was major event during early...
Objectives Inflammatory factors are key mediators of wound healing processes following injury, and their modulation may improve outcomes. The objective this study was to characterize in vivo inflammatory factor extracellular matrix (ECM) messenger RNA (mRNA) expression levels 1 hour after vocal fold injury. Methods Five Sprague-Dawley rats were subjected bilateral 5 reserved as uninjured controls, rat unilateral injury for histology. Tissue harvested Real-time reverse...
Abstract Objective To examine the expressions of fibronectin and other adhesion molecules on scarred vocal folds in a short‐ long‐term animal model. Study Design Animal Methods Six beagles' were stripped unilaterally left untreated. After wounding harvested from three dogs at 2 months 6 months. The untouched fold was used as control, scarred. Subsequently, fibronectin, cadherin, syndecan‐1 syndecan‐4 both examined by immunohistochemical image analysis. Results Compared with control folds,...
Fibrocytes, easily isolated from peripheral blood, hold potential as a cell source for regenerative medicine.
Mouse experimental models are commonly utilized tools in biomedical research but remain underrepresented vocal fold biology, presumably due to the small size of larynx and limited description anatomical, cellular extracellular composition folds. In this study, we provide a whole-mount serial section-based histological morphology wild-type FVB strain mice, alongside immunohistochemical (IHC)-based quantitative analysis matrix (ECM) alteration 1, 7, 14, 28, 42 56 days following unilateral...
Although probable causative agents have been identified (e.g., refluxate components, tobacco smoke), the definitive mechanism for inflammation-related laryngeal mucosal damage remains elusive. Multichannel intraluminal impedance combined with pH monitoring (MII/pH) has emerged as a sensitive tool diagnosis and characterization of gastroesophageal reflux disease laryngopharyngeal manifestations. To determine relationship between signs MII/pH, we examined correlations Reflux Finding Score...