A5044861907- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- Viral Infections and Vectors
- Virus-based gene therapy research
- Invertebrate Immune Response Mechanisms
- Viral Infections and Immunology Research
- Bacteriophages and microbial interactions
- Cellular Mechanics and Interactions
- Vector-Borne Animal Diseases
- Natural Language Processing Techniques
- Retinal Development and Disorders
- Silk-based biomaterials and applications
- SARS-CoV-2 and COVID-19 Research
- Plant Reproductive Biology
- Viral Infections and Outbreaks Research
- Genetics, Bioinformatics, and Biomedical Research
- Neurobiology and Insect Physiology Research
- Cell Image Analysis Techniques
- Retinal Diseases and Treatments
- Genomics and Rare Diseases
- Photoreceptor and optogenetics research
- Phagocytosis and Immune Regulation
- Bioinformatics and Genomic Networks
European Bioinformatics Institute
2024-2025
Pennsylvania State University
2019-2024
Leiden University Medical Center
2021-2024
University of Oxford
2023-2024
Center for Disease Dynamics, Economics & Policy
2022
Helmholtz Moscow Research Institute of Eye Diseases
2021
CSIRO Health and Biosecurity
2015-2018
ACT Government
2017-2018
University of Canberra
2015-2018
Commonwealth Scientific and Industrial Research Organisation
2017-2018
Venezuelan equine encephalitis virus (VEEV) is a representative member of the New World alphaviruses. It transmitted by mosquito vectors and causes highly debilitating disease in humans, equids, other vertebrate hosts. Despite continuous public health threat, very few compounds with anti-VEEV activity cell culture mouse models have been identified to date, rapid development resistance some them has recorded. In this study, we investigated possibility using modified nucleoside analog,...
The aim of the UniProt Knowledgebase (UniProtKB; https://www.uniprot.org/) is to provide users with a comprehensive, high-quality and freely accessible set protein sequences annotated functional information. In this publication, we describe ongoing changes our production pipeline limit available in UniProtKB high-quality, non-redundant reference proteomes. We continue manually curate scientific literature add latest data use machine learning techniques. also encourage community curation...
ABSTRACT Rabbit hemorrhagic disease virus 2 (RHDV2; Lagovirus GI.2) is a pathogenic calicivirus that affects European rabbits ( Oryctolagus cuniculus ) and various hare Lepus species. GI.2 was first detected in France 2010 subsequently caused epidemics wild domestic lagomorph populations throughout Europe. In May 2015, Australia. Within 18 months of its initial detection, had spread to all Australian states territories rapidly became the dominant circulating strain, replacing (RHDV/GI.1)...
The coronavirus papain-like protease (PLpro) is crucial for viral replicase polyprotein processing. Additionally, PLpro can subvert host defense mechanisms by its deubiquitinating (DUB) and deISGylating activities. To elucidate the role of these activities during SARS-CoV-2 infection, we introduced mutations that disrupt binding to ubiquitin or ISG15. We identified several strongly reduced DUB activity PLpro, without affecting In contrast, abrogated also hampered processing when into virus...
Alphaviruses are widely distributed in both hemispheres and circulate between mosquitoes amplifying vertebrate hosts. Geographically separated alphaviruses have adapted to replication particular organisms. The accumulating data suggest that this adaptation is determined not only by changes their glycoproteins but also the amino acid sequence of hypervariable domain (HVD) alphavirus nsP3 protein. We performed a detailed investigation chikungunya virus (CHIKV) HVD interactions with host...
Abstract The highly virulent rabbit hemorrhagic disease virus (RHDV) has been widely used in Australia and New Zealand since the mid-1990s to control wild rabbits, an invasive vertebrate pest these countries. In January 2014, exotic RHDV was detected Australia, 8 additional outbreaks were reported both domestic rabbits 15 months following its detection. Full-length genomic analysis revealed that this is a recombinant containing RHDVa capsid gene nonstructural genes most closely related...
UniProt is a high quality, comprehensive protein resource in which the core activity expert review and annotation of proteins where function has been experimentally investigated. At same time, database contains large numbers are predicted to exist from gene models, but do not have associated experimental evidence indicating their function. commits significant resources developing computational methods for functional these based on data entries that gone through process. We will describe two...
Abstract The UniProt REST API is a freely available, open-access resource that powers the UniProt.org website and gives users flexible programmatic interaction with protein knowledge data. It provides access to UniProtKB, UniRef, UniParc, Proteomes, GeneCentric, ARBA, UniRule, ID Mapping tool, along supporting data controlled vocabularies. Users can their favorite programming language generate example code snippets databases using documentation page...
West Nile virus (WNV) is the leading cause of mosquito-borne illness in USA. There are currently no human vaccines or therapies available for WNV, and vector control primary strategy used to WNV transmission. The Culex tarsalis also a competent host insect-specific (ISV) Eilat (EILV). ISVs such as EILV can interact with superinfection exclusion (SIE) against pathogenic viruses their shared mosquito host, altering competence these viruses. ability SIE restriction make potentially safe tool...
Eilat virus (EILV) is an insect-specific alphavirus that has the potential to be developed into a tool combat mosquito-borne pathogens. However, its mosquito host range and transmission routes are not well understood. Here, we fill this gap by investigating EILV's competence tissue tropism in five species: Aedes aegypti, Culex tarsalis, Anopheles gambiae, stephensi, albimanus. Of tested species, C. tarsalis was most competent for EILV. The found ovaries, but no vertical or venereal observed....
Rabbit haemorrhagic disease virus (RHDV) is a calicivirus that causes acute infections in both domestic and wild European rabbits (Oryctolagus cuniculus). The significant economic losses rabbit farming reduces populations. recent emergence of RHDV variants capable overcoming immunity to other strains emphasises the need develop universally effective antivirals enable quick responses during outbreaks until new vaccines become available. RNA-dependent RNA polymerase (RdRp) primary target for...
Abstract One approach to control dengue virus transmission is the symbiont Wolbachia , which limits viral infection in mosquitoes. Despite plans for its widespread use Aedes aegypti 's mode of action remains poorly understood. Many studies suggest that mechanism likely multifaceted, involving aspects immunity, cellular stress and nutritional competition. A previous study from our group used artificial selection identify a new mosquito candidate gene related blocking; alpha‐mannosidase‐2a (...
Abstract Insect epithelial cells contain cellular extensions such as bristles, hairs, and scales. These are homologous structures that differ in morphology function. They actin bundles dictate their morphology. While the organization, function, identity of major actin-bundling proteins bristles hairs known, this information on scales is unknown. In study, we characterized development role mosquito, Aedes aegypti . We show undergo drastic morphological changes during development, from a...
Recent studies demonstrate that insect-specific viruses can influence the ability of their mosquito hosts to become infected with and transmit arboviruses medical veterinary importance. The aim this study was evaluate interactions between Anopheles gambiae densovirus (AgDNV) (Parvoviridae) (a benign virus infects An. mosquitoes) Mayaro (MAYV) (Togaviridae) (an emerging human pathogen be transmitted by gambiae) in both insect cell culture mosquitoes.For vitro studies, Mos55 cells or...
The extremely pathogenic Rabbit haemorrhagic disease virus (RHDV) and the completely benign calicivirus (RCV) are closely related members of genus Lagovirus (family Caliciviridae). molecular mechanisms that determine dramatic difference in virulence unknown, but indirect evidence suggests different properties their RNA-dependent RNA polymerases (RdRps) may at least partially be responsible for contrasting phenotypes. Here we report unusual ability RHDV RdRp to induce a striking rearrangement...
Abstract Multiple Wolbachia strains can block pathogen infection, replication and/or transmission in Aedes aegypti mosquitoes under both laboratory and field conditions. However, effects on pathogens be highly variable across systems the factors governing this variability are not well understood. It is increasingly clear that mosquito host a passive player which governs phenotypes; rather, genetics of significantly modulate ‐mediated blocking. Specifically, previous work linked variation...
Rabbit hemorrhagic disease virus (RHDV) is a highly contagious calicivirus that causes peracute fever and frequently kills rabbits before an effective adaptive immune response can be developed. In Australia New Zealand, RHDV employed to manage wild European rabbit ( Oryctolagus cuniculus) populations. Although there no evidence replicates in animals other than lagomorphs, the detection of RHDV-specific antibodies RNA mice species has raised concerns about host specificity virus. To...
Rabbit haemorrhagic disease virus (RHDV) is a calicivirus that infects and frequently kills rabbits. Previously, we showed the RHDV RNA-dependent RNA polymerase (RdRp) associated with distinct, but yet uncharacterised subcellular structures capable of inducing redistribution Golgi membranes. In this study, identified partially hidden hydrophobic motif determines localisation recombinant RdRp in transfected cells. This novel motif, 189LLWGCDVGVAVCAAAVFHNICY210, located within F homomorph,...
ABSTRACT West Nile virus (WNV) is the leading cause of mosquito-borne illness in United States. There are currently no human vaccines or therapies available for WNV, and vector control primary strategy used to WNV transmission. The Culex tarsalis also a competent host insect-specific (ISV) Eilat (EILV). ISVs such as EILV can interact with superinfection exclusion (SIE) against pathogenic viruses their shared mosquito host, altering competence these viruses. ability SIE restriction make...