A5069399072- Enzyme Production and Characterization
- Environmental Toxicology and Ecotoxicology
- Enzyme Structure and Function
- Protein Hydrolysis and Bioactive Peptides
- Aquaculture Nutrition and Growth
- Biochemical and Molecular Research
- Phytochemicals and Antioxidant Activities
- Phytase and its Applications
- Biofuel production and bioconversion
- Enzyme-mediated dye degradation
- Plant biochemistry and biosynthesis
- Folate and B Vitamins Research
- Fish biology, ecology, and behavior
- Microbial Natural Products and Biosynthesis
Khon Kaen University
2015-2025
Abstract This study investigated the biochemical properties, enzyme activities, isoenzyme pattern, and molecular weight of three types digestive from six freshwater fish species: Puntius gonionotus (common silver barb), Puntioplites proctozysron (Smith’s Oreochromis niloticus (Nile tilapia), Hemibagrus spilopterus (yellow mystus), Ompok bimaculatus (butter catfish), Kryptopterus geminus (sheatfish). The optimum pHs for amylase alkaline protease activities were 7.0–8.0 8.0–10.0, temperatures...
Cellulase plays an important role in cellulose degradation.The enzyme catalyzes the cleavage of b-1,4 glycosidic bond between glucose residues.The Macrotermes gilvus cellulase was purified by using ammonium sulfate precipitation and anion exchange column with 1.38% recovery 22-fold purification.The SDS-PAGE coupled zymogram analysis revealed molecular weights approximately 54 kDa.The biochemical properties exhibited optimum temperature pH 45°C 5.2.Interestingly, active over a wide range...
Objective: The activity of digestive enzymes and isoenzyme patterns were characterized comparatively studied in seven cyprinid fishes randomly collected from Kongkaew swamp, Khon Kaen province, Thailand.Materials Methods: Fish samples compose Osteochilus hasselti, Labiobarbus spilopleura, Puntius gonionotus, lini, Cyclocheilichthys repasson, apogon brevis.Data analyzed using one-way ANOVA.Results: Different fish species revealed different optimum temperatures pH values for amylase alkaline...
Pyridoxal kinase encoded by pdxK gene, is the important key enzyme in salvage pathway of vitamin B6 biosynthesis. The catalyzes phosphorylation 5′ alcohol groups free form into their 5′-phosphate forms that requires metal ion and ATP. have been reported many organisms except thermophilic bacterium. Therefore, this study aimed to clone, express characterize pyridoxal Geobacillus sp. H6a isolated from hot spring North Thailand. GhpdxK gene (810 base pairs) was inserted pET28a(+) plasmids at...
Pyridoxal 5' -phosphate synthase (PLPS) is present in deoxyxylose 5'-phosphate-independent of the de novo vitamin B6 biosynthesis pathway. This enzyme complex consists PdxS and PdxT, which function as glutamine amidotranferase respectively to produce PLP.This study aimed clone, express, purify PLPS Geobacillus sp. H6a, followed by its characterization.The PdxT genes were amplified from (Gh) H6a. Recombinant vectors pET28a-GhpdxS pET28a-GhpdxT constructed resulting His-tagged proteins...
Chlorpyrifos, one of the organophosphate pesticides, has been widely used in many countries. Binding with soil causes contamination and leaches to freshwater ecosystems. Moreover, uptake accumulation sediment-dwelling organisms leads biomagnification an adverse effect aquatic The studies acute chronic effects chlorpyrifos were investigated here bloodworms, Chironomus pallidinubeculosus. worms reared plastic containers containing sediments supplemented different concentrations (0.1, 1, 10 100...
Chlorpyrifos, one of the organophosphate pesticides, has been widely used in many countries. Binding with soil causes contamination and leaches to freshwater ecosystems. Moreover, uptake accumulation sediment-dwelling organisms leads biomagnification an adverse effect aquatic The chlorpyrifos toxicity on survival rate, acetylcholinesterase activity, enzyme kinetics, mentum deformity bloodworms, Chironomus pallidinubeculosus was investigated. worms were reared plastic containers containing...