A5067748498- Listeria monocytogenes in Food Safety
- CRISPR and Genetic Engineering
- Spectroscopy and Chemometric Analyses
- Microbial Inactivation Methods
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- Meat and Animal Product Quality
- Insect symbiosis and bacterial influences
- Essential Oils and Antimicrobial Activity
- Identification and Quantification in Food
- Evolution and Genetic Dynamics
Wageningen University & Research
2016-2023
A collection of 23 Listeria monocytogenes strains clinical and food origin was tested for their ability to recover grow out in half Fraser enrichment broth following the ISO 11290-1:2017 protocol. Recovery sub-lethally heat-injured cells compared reference with no stress pre-treatment. The enrichments were followed over time by plate counts growth parameters estimated 3-phase model which described data best. without pre-treatment showed a short lag duration, ranged from 1.4 2.7 h. However,...
The dynamics of the enrichment-based detection procedure foodborne pathogen Listeria monocytogenes from food still remains poorly understood. This enrichment is crucial in reliable this and more insight into recovery mechanism during step important to advance our understanding lag phase behaviour enrichment. In study we combined transcriptomic proteomic analyses better understand physiological processes within L. Upon transfer BHI-cultured stationary cells half-Fraser broth (HFB),...
The behaviour of pathogens at the single-cell level can be highly variable and thus affect detection efficacy enrichment-based methods. outgrowth single cells three Listeria monocytogenes strains was monitored after fluorescence-activated sorting in non-selective brain heart infusion (BHI) broth selective half Fraser enrichment (HFB) to quantify heterogeneity its effect on probability. Single-cell higher HFB compared BHI increased further when were heat-stressed. increase also...