Abstract Background Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) continues to be a major economic issue for the swine industry worldwide, not only due acute outbreaks but also endemic infections. PRRS disease severity consequently financial losses can vary greatly between endemically infected farms estimation of damage is challenging. This study aimed assess effect in systematic way at individual farm-level herds, using cost simulation tool. In total 21 German sow herds with...

Human atopic asthma is a complex heritable inflammatory disorder of the airways associated with clinical signs allergic inflammation and airway hyperresponsiveness. Recent studies demonstrate that degree responsiveness strongly interleukin (IL)-9 expression in murine lung. To investigate contribution IL-9 to hyperresponsiveness, explore directly its relationship inflammation, we studied transgenic mice overexpressing IL-9. In this report show (FVB/N-TG5), comparison FVB/NJ mice, display...

Metformin, the popular anti-diabetic drug was shown to exert multiple biological effects. The most recent metformin gained attention as an agent that mobilizes endogenous progenitor cells and enhances regenerative potential of organisms, for example by promoting neurogenesis. In present study, we examined role on mouse olfactory ensheathing (mOECs) derived from animals receiving eight weeks at a concentration equal 2.8 mg/day. mOECs expanded ex vivo were characterized in terms their cellular...

Cryptophycins are a class of 16-membered highly cytotoxic macrocyclic depsipeptides isolated from cyanobacteria. The biological activity is based on their ability to interact with tubulin. They interfere microtubule dynamics and prevent microtubules forming correct mitotic spindles, which causes cell-cycle arrest apoptosis. Their strong antiproliferative activities 100-fold 1000-fold potency compared those paclitaxel vinblastine have been observed. promising drug candidates, as not...

Nowadays, endocrine disorders have become more frequent in both human and veterinary medicine. In horses, reduced physical activity combined with carbohydrate sugar overload may result the development of so-called equine metabolic syndrome (EMS). EMS is characterized by insulin resistance, hyperinsulinemia, elevated blood triglyceride concentrations usually obesity. Although phenotypic features individuals are well known, molecular mechanism underlying disease remains elusive. Therefore,...

Interleukin (IL)-9 is a T helper (Th) 2 cytokine recently implicated as an essential factor in determining susceptibility to asthma. Transgenic mice overexpressing IL-9 exhibit many features that are characteristic of human To better understand the mechanism by which mediates various biologic activities asthma, we performed suppressive subtraction hybridization with whole lung from transgenic and control mice. Here report identification mCLCA3, calcium-activated chloride channel was...

Tumor targeting anticancer drug conjugates that contain a tumor recognition motif (homing device) are of high current relevance. Cryptophycins, naturally occurring cytotoxic cyclo-depsipeptides, have been modified by total synthesis to provide analogues suitable for conjugation peptide-based homing devices. An array functionalized β(2)-amino acids was synthesized and incorporated into cryptophycins. All proved be highly active in the cytotoxicity assay using human cervix carcinoma cell line...

Mesenchymal stem cells (MSCs) are frequently used in both human and veterinary medicine because their unique properties, such as modulating the immune response differentiating into multiple lineages, make them a valuable tool cell-based therapies. However, many studies have indicated age-, lifestyle-, disease-related deterioration of MSC regenerative characteristics. it still needs to be elucidated how patient's health status affects effectiveness differentiation. In present study, we...

The first syntheses of bioactive cryptophycins functionalized at unit D were accomplished in a one-pot Staudinger reduction/cyclization step. An azido precursor for the lower part backbone was introduced to minimize protective group chemistry and enable very convenient synthesis cryptophycin-52 cryptophycin analogues containing an ester or free carboxylic acid bioconjugations. Both new derivatives show high biological activity cytotoxicity assays.

This study investigated in vitro effects of freshwater alga Cladophora glomerata water extract enriched during a biosorption process Cr(III) trivalent chromium and picolinate on adipose-derived mesenchymal stromal stem cells (ASCs) extracellular microvesicles (MVs) equine metabolic syndrome-affected horses. Chemical characterisation natural was performed with special emphasis on: vitamin C, E, total phenols, fatty acids, free protein-bound amino acids as well measured Cr algal biomass. To...

We have previously shown that expression of a Ca2+-activated Cl- channel (mCLCA3 in mice and bCLCA1 humans) is up-regulated along with goblet cell metaplasia mucus overproduction the lungs interleukin 9 (IL9) transgenic mice, human primary lung cultures by IL4, IL13 IL9. show here hCLCA1 NCI-H292 cells specifically induces soluble gel-forming mucin production. Moreover, niflumic acid (NFA), blocker hCLCA1-dependent efflux, inhibits MUC5A/C production these cells. NFA treatment during natural...

Surgical castration of male piglets without analgesia is a painful procedure. This prospective, randomized and double-blinded study aimed to evaluate the analgesic effects four different local anesthetics for piglet during first week life. In total, 54 aged 3 7 days were distributed into 6 treatment groups: handling (H); pain relief (sodium chloride, NaCl); with anesthetic: 4% procaine (P), 2% lidocaine (L), 0.5% bupivacaine (B) or 20 mg/ml mepivacaine (M). By excluding stress fear as...

Castration of male pigs without anesthesia is a significant welfare issue. Improvac®, GnRH vaccine induces an endogenous immune response leading to decrease in testicular steroids. Consequences different vaccination schemes on function and carcass quality were evaluated immunocastrated boars (IC), surgical castrates (SC), entire males (EM). Therefore, 128 piglets assigned five treatment-groups long term follow-up group. IC groups received two vaccinations (V1, V2) with Improvac® at 8 12, 12...

Abstract Endocrine disorders have become more and frequently diagnosed in humans animals. In horses, equine metabolic syndrome ( EMS ) is characterized by insulin resistance, hyperleptinemia, hyperinsulinemia, inflammation usually pathological obesity. Due to an increased inflammatory response the adipose tissue, cytophysiological properties of derived stem cells ASC been impaired, which strongly limits their therapeutic potential. Excessive accumulation reactive oxygen species, mitochondria...

The aim of the present study was to investigate effect four local anesthetics on pain relief during surgical castration under standardized conditions in conscious piglets. Therefore, 71 male piglets (three seven days) were distributed into control groups (handling, without anesthesia or analgesia) and anesthetic trial (procaine, lidocaine, bupivacaine, mepivacaine). Then, 20 min prior castration, animals treatment groups, except handling group, received an injection a sodium chloride 0.5 mL...

Protein tyrosine phosphatase PTP1B is considered as a key metabolic enzyme that has been reported to be associated with insulin resistance onset, and underlying cellular malfunctions, including ER stress mitochondrial failure. In this study, effects of selective inhibition using MSI-1436 on apoptosis, oxidative stress, dysfunction have assessed an in vitro model Tunicamycin induced HepG2 cell line. Inhibition significantly increased viability reduced the number apoptotic cells well...

Journal Article On the Toxic Substances from Virulent Pneumococci and Pneumonic Lungs Influence Thereon of Quinin Urea Hydrochlorid, Ethylhydrocuprein Other Cinchona Derivatives: Studies in Pneumonia, VI Get access S. Solis-Cohen, Solis-Cohen McManes Laboratory Experimental Pathology University Pennsylvania, Philadelphia Search for other works by this author on: Oxford Academic PubMed Google Scholar Charles Weiss, Weiss John A. Kolmer The Infectious Diseases, Volume 22, Issue 5, May 1918,...

A chiral diborate with different counterions is reported whose structure was unambiguously confirmed by X-ray analysis. This dianion used in the resolution of trans-1,2-diamines and also as a shift reagent for NMR analysis triphenylphosphonium salts.

Extracellular vesicles (EVs), a spherical membrane fragments including exosomes, are released from several cell types, mesenchymal stromal cells (MSCs), constitutively or under stimulation. As MVs cargo include DNA, RNA, miRNA, lipids and proteins their have gain special attention in the field of regenerative medicine. Depending on type transferred molecules, may exert wide range biological effects recipient pro-inflammatory anti-apoptotic action. In presented paper, we isolated form adipose...

Cryptophycins are cytotoxic natural products that exhibit considerable activities even against multi-drug-resistant tumor cell lines. As fluorinated pharmaceuticals have become more and important during the past decades, fluorine-functionalized cryptophycins were synthesized evaluated in cell-based cytotoxicity assays. The unit A trifluoromethyl-modified cryptophycin proved to be highly active KB-3-1 cells exhibited an IC(50) value low picomolar range. However, replacement of...

Recently, metabolic syndrome (MS) has gained attention in human and animal medicine. Insulin resistance, inflammation, hyperleptinemia, hyperinsulinemia are critical to its definition. MS is a complex cluster of risk factors that together exert wide range effects on multiple organs, tissues, cells the body. Adipose stem (ASCs) multipotent cell population residing within adipose tissue inflamed during MS. Studies have indicated these lose their stemness multipotency MS, which strongly reduces...

Selecting a well-suited method for isolating/characterizing circulating tumor cells (CTCs) is challenging. Evaluating sensitive and specific markers prostate cancer (PCa)-specific CTC identification analysis crucial. We used the CellCollector EpCAM-functionalized system (CC-EpCAM) evaluated developed PCa-functionalized version (CC-PCa); we then compared isolation techniques that exploit physical biological properties of CTCs. established two cohorts metastatic PCa patients (mPCa; 15 in...