A5083256966- Cell Adhesion Molecules Research
- Collagen: Extraction and Characterization
- Osteoarthritis Treatment and Mechanisms
- Proteoglycans and glycosaminoglycans research
- Connective tissue disorders research
- Developmental Biology and Gene Regulation
- RNA Research and Splicing
- dental development and anomalies
- Hedgehog Signaling Pathway Studies
- Protease and Inhibitor Mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cleft Lip and Palate Research
- Platelet Disorders and Treatments
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Chemical Synthesis and Analysis
- Angiogenesis and VEGF in Cancer
- Pluripotent Stem Cells Research
- AI in cancer detection
- Craniofacial Disorders and Treatments
- Corneal surgery and disorders
- Cancer-related gene regulation
- Bone and Dental Protein Studies
- Inflammatory mediators and NSAID effects
- Mitochondrial Function and Pathology
National Institute of Informatics
2020-2024
Research Organization of Information and Systems
2015-2024
National Institute of Genetics
2015-2019
Okayama University
1996-2009
University of Oxford
2005-2007
Tokyo Medical and Dental University
1980-2003
Shigei Medical Research Institute
1997-1998
Cedars-Sinai Medical Center
1998
Montgomery General Hospital
1998
Hôpital de la Timone
1994
Genes for the human alpha 5(IV) and 6(IV) collagen chains have a unique arrangement in that they are colocalized on chromosome Xq22 head-to-head fashion appear to share common bidirectional promoter. In addition we reported novel observation COL4A6 gene is transcribed from two alternative promoters tissue-specific manner (Sugimoto, M., T. Oohashi, Y. Ninomiya. 1994. Proc. Natl. Acad. Sci. USA. 91:11679-11683). To know whether translation products of both genes various tissues, raised...
Type X collagen is a recently discovered product of hypertrophic chondrocytes that localized to presumptive mineralization zones hyaline cartilage. Thus, in the epiphyseal growth plate long bones it present only zone and absent resting rapidly growing cartilage bone. represents, therefore, transient developmentally regulated which synthesized by subpopulation chondrocytes. We report here isolation characterization cDNA genomic clones specific for chicken protein. The results demonstrate...
We present a detailed analysis both of tryptic peptides and amino-terminal sequences the subunits two collagenous fragments (HMW LMW) previously isolated from pepsin extracts chicken cartilage (Reese, C.A., Mayne, R. (1981) Biochemistry 20, 5443-5448). This comparison with nucleotide sequence cDNApYN1738 (Ninomiya, Y., Olsen, B.R. (1984) Proc. Natl. Acad. Sci. U. S. A. 81, 3014-3018) shows that HMW LMW are pepsin-resistant unique collagen composed molecules three different polypeptide chains...
ABSTRACT This study investigates the migration patterns of cranial neural crest cells in retinoic acid (RA)-treated rat embryos using DiI labeling. Wistar-Imamichi were treated at early (9.0 days post coitum, d.p.c.) and late (9.5 plate stages with all-trans RA (2×10-7 M) for 6 hours further cultured an RA-free medium. exposure stage dependently induced two typical cran-iofacial abnormalities; that is, 9.0 d.p.c. it reduced size shape first branchial arch to those second arch, whereas,...
Vascular and parenchymal basement membranes (BMs) are thickened in diabetes, but alterations individual BM components diabetic eyes, especially retinopathy (DR), obscure. To identify abnormalities the distribution of specific constituents, we analyzed cryostat sections human eyes obtained at autopsy (seven normal, five without DR, 13 with DR) by immunofluorescence antibodies to 30 extracellular matrix components. In non-DR no qualitative changes ocular were seen. some DR corneas, epithelial...
We have isolated several overlapping cDNA clones encoding alpha 1(XI) collagen chains from human and rat libraries. Together the cDNAs code for 335 uninterrupted Gly-X-Y triplets, a 264-amino acid C-propeptide, while cover entire C-propeptide about third of triple-helical domain. Comparison rodent nucleotide sequences showed 95% sequence similarity. The identification as was based on complete identity between amino three cyanogen bromide peptides cDNA-derived sequence. Examination variety...
Hyaline cartilage contains a unique set of collagenous proteins. Type II collagen is the most abundant, constituting about 85% total collagen. In addition, several minor components have been described. To study structure and developmental regulation chondrocyte-specific collagens, we constructed cDNA library from embryonic chicken sternal mRNA. We report here on isolation characterization 3200 base-pair-long that codes for polypeptide unusual in length molecule only half pro alpha 1(II)...
We have employed a highly specific in situ hybridization protocol that allows differential detection of mRNAs collagen types I and II paraffin sections from chick embryo tissues. All probes were cDNA restriction fragments encoding portions the C-propeptide region pro alpha-chain, some also encoded 3'-untranslated either type or collagen. Smears tendon fibroblasts those sternal chondrocytes 17-d-old embryos as well 10-d-old whole cornea 6.5-d-old hybridized with 3H-labeled for mRNA....
Abstract We have isolated two overlapping cDNA clones covering 2425 base pairs encoding a short type VIII collagen chain synthesized by rabbit corneal endothelial cells. The cDNAs encode an open reading frame of 744 amino acid residues containing triple-helical domain 454 flanked 117- and 173-residue carboxyl non-triple-helical domains (called NC2 NC1, respectively). Based on the identity between DNA-derived sequence CNBr peptide obtained from Descemet's membrane, we conclude that code for...
Type IX collagen represents 5-20% of the total in hyaline cartilage. The molecules this are composed three genetically distinct polypeptide subunits. One these subunits, alpha 2(IX), contains covalently bound glycosaminoglycan (chondroitin sulfate or dermatan sulfate). We report here on structure attachment site type collagen-proteoglycan. show, by a combination cDNA and peptide sequencing, that region sequence Gly-Ser-Ala-Asp, located within noncollagenous domain NC3 2(IX) chain. By...
Type IX collagen in cartilage consists of molecules composed three genetically distinct polypeptide subunits. One the subunits, alpha 2(IX), contains a covalently attached glycosaminoglycan side chain whereas second subunit, 1(IX), large noncollagenous, amino-terminal domain called NC4. In this report, we describe for first time complete primary structure noncollagenous domain, based on cloning and sequencing cDNA genomic DNA as well amino acid tryptic peptides. Analysis clones has also...
We have isolated three overlapping cDNA clones encoding the pro alpha 2(XI) collagen chain from a human chondrocyte library. Together, cDNAs code for 257 uninterrupted Gly-X-Y triplets (almost 80% of triple helical domain) and about 200 amino acid residues carboxyl telopeptide propeptide. The identification as was based on complete identity between sequences tryptic peptides derived cDNA-derived sequence. also sequenced six exons within genomic cosmid clone. This sequence shows that although...
We have analyzed embryonic chicken cornea for the presence of type IX collagen mRNA and protein. Using RNA transfer blot analysis, we demonstrate that alpha 1(IX) 2(IX) mRNAs are expressed by corneal epithelial cells at time primary stromal components synthesized. The levels decrease with increasing developmental age barely detectable day 11 development. In contrast, protein is immunofluorescence days 5 6 undetectable 8. probes specific mRNAs, size same in as chondrocytes, major source...
This study examined whether the capsule which encases islets of Langerhans in NOD mouse pancreas represents a specialised extracellular matrix (ECM) or basement membrane that protects from autoimmune attack.Immunofluorescence microscopy using panel antibodies to collagens type IV, laminins, nidogens and perlecan was performed localise components before diabetes onset, at onset after clinical established (2-8.5 weeks post-onset).Perlecan, heparan sulphate proteoglycan is characteristic...
Type X collagen, expressed by hypertrophic chondrocytes, consists of homotrimeric molecules with subunits that are only about one-half the size polypeptides fibrillar collagens. In this report we describe for first time complete primary structure type based on cloning and sequencing cDNA genomic DNA. A comparison between nucleotide sequences DNA clones has also allowed determination exon collagen gene. Our results demonstrate translation product chicken mRNA is 682 amino acid residues long a...
To date, five distinct a chains have been identified in basement membrane collagen IV.W e cloned gene encoding new chain belonging to IV by cDNA isolation under low stringency conditions.Isolation of overlapping clones and the technique 6' rapid amplification ends enabled us derive primary structure entire polypeptide.The deduced polypeptide contained 1678 amino acid residues, including 21-residue signal peptide, 24-residue amino-terminal NC domain, central 1406-residue collagenous (COL1)...
Type VIII collagen is a major component of Descemet's membrane, the specialized basement membrane corneal endothelial cells. Sequence analysis cDNA isolated from library made with mRNA rabbit cells has indicated that type molecules contain polypeptide chain, alpha 1(VIII), consisting short triple-helical domain 454 amino acid residues flanked by non-triple-helical domains 117 and 173 at carboxyl ends, respectively (Yamaguchi, N., Benya, P. D., van der Rest, M., Ninomiya, Y. (1989) J. Biol....