A5036675361- Nanoparticles: synthesis and applications
- Inhalation and Respiratory Drug Delivery
- Air Quality and Health Impacts
- 3D Printing in Biomedical Research
- Ion-surface interactions and analysis
- Biocrusts and Microbial Ecology
- Microplastics and Plastic Pollution
- Heavy Metal Exposure and Toxicity
- Effects and risks of endocrine disrupting chemicals
- Wound Healing and Treatments
- Bone Tissue Engineering Materials
- Microbial Metabolism and Applications
LMU Klinikum
2022
Ludwig-Maximilians-Universität München
2022
Federal Institute for Risk Assessment
2018-2021
Abstract Inhalation is the most relevant entry point for nanoparticles (NPs) into human body. To date, toxicity testing of nanomaterials in respect to oral, dermal and inhalative application mainly based on animal experiments. The development alternative test methods subject current research. In vitro models can help investigate mechanistic aspects, as e.g. cellular uptake or genotoxicity might reduce vivo testing. Lung cell lines are proper tools assess NP toxicity. this, various have been...
Abstract The biomolecular imaging of cell-nanoparticle (NP) interactions using time-of-flight secondary ion mass spectrometry (ToF-SIMS) represents an evolving tool in nanotoxicology. In this study we present the three dimensional (3D) distribution nanomaterials within agglomerates ToF-SIMS imaging. This novel approach was used to model resistance human alveolar A549 cells against gold (Au) toxicity through intra- and extracellular biomineralization. At low Au concentrations (≤1 mM HAuCl 4 )...
Air–liquid interface (ALI) systems have been widely used in recent years to investigate the inhalation toxicity of many gaseous compounds, chemicals, and nanomaterials represent an emerging promising vitro method supplement vivo studies. ALI exposure reflects physiological conditions deep lung more closely subacute scenarios compared submerged exposure. The comparability toxicological results obtained from data is still challenging. robustness not yet well understood, but critical for...
In vitro inhalation toxicology methods are increasingly being used for research and regulatory purposes. Although the opportunity increased human relevance of in compared to vivo tests has been established discussed, how systematically account variability maximize reliability these methods, especially assays that use cells cultured at an air–liquid interface (ALI), received less attention. One tool evaluate robustness test is cause-and-effect (C&E) analysis, a conceptual approach analyze key...
Inhalation Toxicology In article number 2100633 by Ajay Vikram Singh, Byung-Wook Park, and co-workers, a systematic approach is taken to discuss the bottleneck, pros cons of current technologies pertaining organ-on-a-chip systems for inhalation toxicology. Design processes inspired from soft robotics actuation with attention exposure, lung cells, materials used are presented.
Dermal replacement materials bioactivated with cyanobacteria have shown promising potential for wound regeneration. To date, extraction of RNA from seeded scaffolds has not been described. The aim this study was to develop a method isolate total and propose new approach in determining living bacteria based on real-time PCR. Transgenic Synechococcus sp. PCC 7002 (tSyn7002) were liquid cultures or dermal regeneration vitro vivo 7 days. extracted 260/280 ratio ≥2. small subunit the 30S ribosome...