A5101987328- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Polysaccharides and Plant Cell Walls
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant Pathogenic Bacteria Studies
- Plant Physiology and Cultivation Studies
- Plant Micronutrient Interactions and Effects
- Plant biochemistry and biosynthesis
- Plant responses to water stress
- Plant Parasitism and Resistance
- Insect Pest Control Strategies
- Plant Gene Expression Analysis
- Garlic and Onion Studies
- Plant pathogens and resistance mechanisms
- Legume Nitrogen Fixing Symbiosis
- GABA and Rice Research
- Genetic and Environmental Crop Studies
- Seed Germination and Physiology
- Plant Genetic and Mutation Studies
- Plant Disease Resistance and Genetics
- Insect-Plant Interactions and Control
- Polysaccharides Composition and Applications
U.S. Salinity Laboratory
2020-2024
University of California, Riverside
2020-2024
Pennsylvania State University
2008-2019
Donald Danforth Plant Science Center
2016-2017
Syracuse University
2007-2014
Degrees of Separation Proteins embedded in membranes represent an interesting point communication between the cell and its environment, but their localization to can make them difficult study. Jones et al. (p. 711 ) found approach catalog thousands interactions involving membrane proteins membrane-associated signaling machinery—including many previously uncharacterized proteins. With a focus on model plant Arabidopsis , several identified fill gaps important signal transduction chains, while...
Summary Currently, there are strong inconsistencies in our knowledge of plant heterotrimeric G‐proteins that suggest the existence additional members family. We have identified a new Arabidopsis G‐protein γ‐subunit (AGG3) modulates morphological development and ABA‐regulation stomatal aperture. AGG3 strongly interacts with β‐subunit vivo vitro . Most importantly, AGG3‐deficient mutants account for all but one ‘orphan’ phenotypes previously unexplained by two known γ‐subunits Arabidopsis. has...
Summary Open Stomata 1 ( OST 1) (Sn RK 2.6 or SRK 2E), a serine/threonine protein kinase, is positive regulator in abscisic acid (ABA) ‐mediated stomatal response, but 1‐regulation of K + and Ca 2+ currents has not been studied directly guard cells it unknown whether activity limiting ABA responses. We employed loss‐of‐function gain‐of‐function approaches to study native responses Arabidopsis cells. performed aperture bioassays, patch clamp analyses reactive oxygen species ROS )...
Summary Protein kinases play important roles in relaying information from perception of a signal to the effector genes all organisms. Cysteine‐rich receptor‐like (CRKs) constitute sub‐family plant (RLKs) with more than 40 members that contain novel C‐X8‐C‐X2‐C motif (DUF26) extracellular domains. Here we report molecular characterization one member this gene family, CRK13 . Expression is induced quickly and strongly response avirulent compared virulent strains Pseudomonas syringae , peaks...
Interactions between membrane proteins and the soluble fraction are essential for signal transduction regulating nutrient transport. To gain insights into membrane-based interactome, 3,852 open reading frames (ORFs) out of a target list 8,383 representing signaling from Arabidopsis thaliana were cloned Gateway compatible vector. The mating-based split-ubiquitin system was used to screen potential protein-protein interactions (pPPIs) among 490 ORFs. A binary robotic 142 receptor-like kinases,...
In Arabidopsis, the GH3-like gene family consists of 19 members, several which have been shown to adenylate plant hormones jasmonic acid, indole acetic acid and salicylic (SA). some cases, this adenylation has catalyze hormone conjugation amino acids. Here we report molecular characterization GH3-LIKE DEFENSE GENE 1 (GDG1), a member family, show that GDG1 is an important component SA-mediated defense against bacterial pathogen Pseudomonas syringae. Expression induced earlier higher level in...
Guard cells shrink in response to drought and abscisic acid (ABA), which is caused by efflux of ions that turn reduces stomatal aperture improves the plant's ability retain moisture. Cytosolic free calcium an essential secondary messenger guard cell ABA signaling, but details this regulatory pathway remain sketchy. Here, calmodulin-like protein CML20, has four EF-hand domains calcium-binding activity vitro, was found be a negative regulator ABA-induced movement Arabidopsis. The cml20...
Stomata, microscopic pores in leaf surfaces through which water loss and carbon dioxide uptake occur, are closed response to drought by the phytohormone abscisic acid (ABA). This process is vital for tolerance has been topic of extensive experimental investigation last decades. Although a core signaling chain elucidated consisting ABA binding receptors, alleviates negative regulation protein phosphatases 2C (PP2Cs) kinase OPEN STOMATA 1 (OST1) ultimately results activation anion channels,...
Plant cell walls undergo dynamic structural and chemical changes during plant development growth. Floral organ abscission lateral root emergence are both accompanied by cell-wall remodeling, which involves the INFLORESCENCE DEFICIENT IN ABSCISSION (IDA)-derived peptide its receptors, HAESA (HAE) HAESA-LIKE2 (HSL2). also act as barriers against pathogenic invaders. Thus, remodeling could have an influence on resistance to phytopathogens. Here, we identified IDA-like 6 (IDL6), a gene that is...
Abstract The concentration alteration of cytosolic‐free calcium ([ Ca 2+ ] cyt ) is a well‐known secondary messenger in plants and plays important roles during pollen grain germination tube elongation. Here we demonstrate that CML 25, member calmodulin‐like proteins, has ‐binding activity role germination, elongation seed setting. 25 transcript was abundant mature grains tubes, its product protein primarily directed to the cytoplasm. Two independent loss‐of‐function T‐DNA insertion mutants...
Abstract The almond crop has high economic importance on a global scale, but its sensitivity to salinity stress can cause severe yield losses. Salt-tolerant rootstocks are vital for feasibility under saline conditions. Two commercial submitted salinity, and evaluated through different parameters, had contrasting results with the survival rates of 90.6% ‘Rootpac 40’ (tolerant) 38.9% ‘Nemaguard’ (sensitive) (Electrical conductivity water = 3 dS m −1 ). Under accumulated less Na Cl more K in...
Guar is a commercially important legume crop known for guar gum. tolerant to various abiotic stresses, but the mechanisms involved in its salinity tolerance are not well established. This study aimed understand molecular of guar. RNA sequencing (RNA-Seq) was employed leaf and root transcriptomes salt-tolerant (Matador) salt-sensitive (PI 340261) genotypes under control salinity. Our analyses identified total 296,114 unigenes assembled from 527 million clean reads. Transcriptome analysis...
Abstract Salinity is a major abiotic stress factor that can significantly impact crop growth, and productivity. In response to salt stress, the plant Salt Overly Sensitive (SOS) signaling pathway regulates homeostasis of intracellular sodium ion concentration. The SOS1 , SOS2 SOS3 genes play critical roles in SOS pathway, which belongs members Na + /H exchanger ( NHX ), CBL‐interacting protein kinase CIPK calcineurin B‐like CBL ) gene families, respectively. this study, we performed...
Abstract Fourteen commercial almond rootstocks were tested under five types of irrigation waters to understand the genetic, physiological, and biochemical bases salt-tolerance mechanisms. Treatments included control (T1) four saline water treatments dominant in sodium-sulfate (T2), sodium-chloride (T3), sodium-chloride/sulfate (T4), calcium/magnesium-chloride/sulfate (T5). T3 caused highest reduction survival rate trunk diameter, followed by T4 T2, indicating that Na and, a lesser extent, Cl...
High temperature induces stomatal opening; however, uncontrolled opening is dangerous for plants in response to high temperature. We identified a high-temperature sensitive (hts) mutant from the ethyl methane sulfonate (EMS)-induced maize (Zea mays) library that linked single base change MITOGEN-ACTIVATED PROTEIN KINASE 20 (ZmMPK20). Our data demonstrated hts mutants exhibit substantially increased and water loss rate, as well decreased thermotolerance, compared wild-type under...
Setaria viridis (green foxtail) is an important model plant for the study of C4 photosynthesis in panicoid grasses, and fast emerging as a system choice development, domestication, abiotic stress responses evolution. Basic research findings are expected to advance not only this species its close relative S. italica (foxtail millet), but also other many which food or bioenergy crops. Here we report on standardization multiple growth development assays under controlled conditions, response...
Cytosolic calcium concentration ([Ca2+ ]cyt ) and heterotrimeric G-proteins are universal eukaryotic signaling elements. In plant guard cells, extracellular (Cao is as strong a stimulus for stomatal closure the phytohormone abscisic acid (ABA), but underlying mechanisms remain elusive. Here, we report that sole Arabidopsis Gβ subunit, AGB1, required four cell Cao responses: induction of closure; inhibition opening; [Ca2+ oscillation; inositol 1,4,5-trisphosphate (InsP3) production. Stomata...
Abstract Alfalfa is an important forage crop that moderately tolerant to salinity; however, little known about its salt-tolerance mechanisms. We studied root and leaf transcriptomes of a salt-tolerant (G03) salt-sensitive (G09) genotype, irrigated with waters low high salinities. RNA sequencing led 1.73 billion high-quality reads were assembled into 418,480 unigenes; 35% which assigned 57 Gene Ontology annotations. The unigenes pathway databases for understanding high-level functions....
Drought causes a major constraint on plant growth, development, and crop productivity. stress enhances the synthesis mobilization of phytohormone abscisic acid (ABA). Enhanced cellular levels ABA promote production reactive oxygen species (ROS), which in turn induce anion channel activity guard cells that consequently leads to stomatal closure. Although Cyclophilins (CYPs) are known participate biotic response, their involvement cell signaling drought response remains be established. The...
Plant stomata which consist of a pair guard cells, are not only finely controlled to balance water loss as transpiration and CO2 absorption for photosynthesis, but also serve the major sites defend against pathogen attack, thus allowing plants respond appropriately abiotic biotic stress conditions. The regulatory signaling network stomatal movement is complex in nature, plant peptides have been shown be involved processes. Arabidopsis secreted peptide PIP1 was previously identified an...
Plants use multiple interacting signaling systems to identify and respond biotic stresses. Although it is often assumed that there specificity in responses specific pests, this rarely examined outside of the gene-for-gene relationships plant-pathogen interactions. In study, we first compared early events gene expression later metabolite profiles Arabidopsis thaliana following attack by either caterpillar Spodoptera exigua or avirulent (DC3000 avrRpm1) Pseudomonas syringae pv. tomato at three...