A5101656993- Bacillus and Francisella bacterial research
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Outbreaks Research
- Viral gastroenteritis research and epidemiology
- Erythrocyte Function and Pathophysiology
- DNA Repair Mechanisms
- Extracellular vesicles in disease
- Phagocytosis and Immune Regulation
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Biomarkers in Disease Mechanisms
- Microbial infections and disease research
- Diabetes and associated disorders
- Poxvirus research and outbreaks
- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Chemical Synthesis and Analysis
- Fluorine in Organic Chemistry
University of Minnesota Rochester
2020
University of Pittsburgh
2009-2019
Johns Hopkins University
2019
Pediatrics and Genetics
2009
University of Cincinnati
2005
Section:ChooseTop of pageAbstract <<MATERIALS AND METHODSRESULTSDISCUSSIONReferencesCITING ARTICLES
Tularemia is a debilitating febrile illness caused by the category A biodefense agent Francisella tularensis. This pathogen infects over 250 different hosts, has low infectious dose, and causes high morbidity mortality. Our understanding of mechanisms which F. tularensis senses adapts to host environments incomplete. Polyamines, including spermine, regulate interactions with cells. However, it not known whether responsiveness polyamines necessary for virulence organism. Through transposon...
Francisella tularensis is the causative agent of debilitating febrile illness tularemia. The severe morbidity associated with F. infections attributed to its ability evade host immune response. Innate activation undetectable until more than 48 hours after infection. ensuing inflammatory response considered pathological, eliciting a septic-like state characterized by hypercytokinemia and cell death. To investigate potential pathological consequences innate response, mice deficient in key...
Vaccination is a proven strategy to mitigate morbidity and mortality of infectious diseases. The methodology identifying testing new vaccine candidates could be improved with rational design in vitro prior animal experimentation. tularemia vaccine, Francisella tularensis live strain (LVS), does not elicit complete protection against lethal challenge virulent type A strain. One factor that may contribute this poor performance limited stimulation antigen-presenting cells. In study, we examined...
In the absence of arabinose, dimeric Escherichia coli regulatory protein l-arabinose operon, AraC, represses expression by looping DNA between distant half-sites. Binding arabinose to dimerization domains forces AraC preferentially bind two adjacent half-sites, which stimulates RNA polymerase transcription araBAD catabolism genes. Prior genetic and biochemical studies hypothesized that allosterically induces a helix-coil transition linker binding switches conformation an inducing state...
In Escherichia coli, the dimeric AraC protein actively represses transcription from l-arabinose araBAD operon in absence of arabinose but induces its presence. Here we provide evidence that, shifting repressing to inducing state, behavior interdomain linker shifts that an α helix a more flexible form. vivo and vitro experiments show with sequence favors formation is shifted toward state presence arabinose. Conversely, containing unfavorable for state. Experiments which presumed helical...
Pathogens enhance their survival during infections by manipulating host defenses. Francisella tularensis evades innate immune responses, which we have found to be dependent on an understudied gene ybeX (FTL_0883/FTT_0615c). To understand the function of YbeX, sought protein interactors in F. subsp. holarctica live vaccine strain (LVS). An unstudied co-immunoprecipitated with recombinant is a predicted glycosyltransferase DXD-motif. There are up four genomic copies this identical sequence...
The title complex, C14H13BrClNO, adopts a helical motif built up from intermolecular O—H⋯O interactions with repeat distance of 8.4 Å. Further assembly the helices forms parallel square grid array reminiscent apolar peptide extended packing motifs.
Coordinated activity among islet cells is necessary for glucose homeostasis. Chronic exposure to diabetogenic factors such as pro-inflammatory cytokines, perturbs cell crosstalk and β-cell function in diabetes. Secretory nanovesicles - exosomes (EXO) derived from cytokine exposed β-cells modulate physiological pathological responses stress. However, the mechanisms governing this process remain unknown. We tested hypothesis that failure diabetes mediated part through autocrine release of EXO...