A5080961456- Lymphoma Diagnosis and Treatment
- Multiple Myeloma Research and Treatments
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Drug Transport and Resistance Mechanisms
- Hematopoietic Stem Cell Transplantation
- Acute Lymphoblastic Leukemia research
- Hemoglobinopathies and Related Disorders
- Peptidase Inhibition and Analysis
- Viral-associated cancers and disorders
- Proteoglycans and glycosaminoglycans research
- Ubiquitin and proteasome pathways
- Neutropenia and Cancer Infections
- Immunodeficiency and Autoimmune Disorders
- NF-κB Signaling Pathways
- Cancer-related Molecular Pathways
- Monoclonal and Polyclonal Antibodies Research
- Renal Diseases and Glomerulopathies
- Glycosylation and Glycoproteins Research
- Histone Deacetylase Inhibitors Research
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
- Blood disorders and treatments
- DNA Repair Mechanisms
- Autoimmune Bullous Skin Diseases
University of Wisconsin–Madison
2009-2023
UW Health University Hospital
2020
University of Wisconsin System
2010-2019
Wisconsin Division of Public Health
2016
Advanced Bioscience Laboratories (United States)
2016
Centers for Disease Control and Prevention
2016
Massachusetts General Hospital
2013
University of Vermont
2013
Virginia Commonwealth University
2013
Mount Sinai Hospital
2013
Abstract Bortezomib (Velcade/PS341), a proteasome inhibitor used in the treatment of multiple myeloma (MM), can inhibit activation nuclear factor-κB (NF-κB), family transcription factors often deregulated and constitutively activated primary MM cells. NF-κB be via several distinct mechanisms, including inhibitor–resistant (PIR) pathway. It remains unknown what fraction cells harbor constitutive activity maintained by proteasome-dependent mechanisms. Here, we report an unexpected finding that...
Abstract Background Components of the microenvironment such as bone marrow stromal cells (BMSCs) are well known to support multiple myeloma (MM) disease progression and resistance chemotherapy including proteasome inhibitor bortezomib. However, functional distinctions between BMSCs in MM patients those disease-free not completely understood. We other investigators have recently reported that NF-κB activity primary is largely resistant bortezomib, further enhancement by similarly bortezomib...
Distinction of normal B-lymphoid proliferations including precursors known as hematogones from acute lymphoblastic leukemia (ALL) is critical for disease management. We present a multiparameter assessment 27 bone marrow samples containing at least 25% (range, 25%-72%) by morphologic review. used flow cytometry to evaluate B-cell differentiation antigen and adhesion molecule expression immunohistochemistry on clot sections architectural distribution. Flow revealed that intermediately...
Babesia microti, an intraerythrocytic parasite, is tickborne in nature. In contrast to transmission by blood transfusion, which has been well documented, associated with solid organ transplantation not reported. We describe parasitologically confirmed cases of babesiosis diagnosed ≈8 weeks posttransplantation 2 recipients renal allografts from donor who was multiply transfused on the day he died traumatic injuries. The and had no identified risk factors for infection. Antibodies against B....
Peripheral smear review is a critical, but labor intensive adjunct for evaluation of lymphocytosis. Standard practice based on consensus guidelines to cases with absolute lymphocyte count (ALC) >5×109/L. We hypothesize that identifying by applying appropriately adjusted ALC and age discriminators will decrease laboratory workload without compromising patient care.1170 complete blood counts ALCs >5×109/L analyzed in the core during 2-year period were included. Patients categorized into...
Early detection of relapse in children with acute lymphoblastic leukemia (ALL), as well distinction leukemic blasts from hematogones, can be difficult by morphologic examination alone. Using CD34 and terminal deoxynucleotidyl transferase (TdT) immunoperoxidase stains, we studied specimens 25 ALL remission to determine if could identify at risk relapse. We bone marrow 9 patients who experienced during the subsequent 6 months 16 remained complete remission, including 10 increased numbers...
Abstract Purpose: This phase I trial assessed the safety and tolerability of G3139 when given in combination with carboplatin paclitaxel chemotherapy. The effect treatment on Bcl-2 expression peripheral blood mononuclear cells (PBMC) paired tumor biopsies was also determined. Experimental Design: Patients advanced solid malignancies received various doses (continuous i.v. infusion days 1-7), (day 4), repeated 3-week cycles, a standard cohort-of-three dose-escalation schema. Changes Bcl-2/Bax...
Flow cytometry is essential for the evaluation of lymphoproliferative disorders (LPDs) and their classification. panels routinely incorporate a large array antibodies, making testing complex expensive; such are likely unnecessary in benign cases or those with straightforward diagnoses. Our aim was to develop more cost-effective strategy based on retrospective analysis flow studies possible LPDs blood.We identified LPD frequencies types, as well associated results patient age absolute...
Waldenstrom macroglobulinemia is a low-grade B-cell lymphoproliferative disorder of the elderly with characteristic monoclonal IgM-producing neoplastic infiltrates bone marrow, lymph node, and spleen. Cutaneous manifestations are usually nonspecific such as purpura, ulcers, urticarial lesions. These lesions caused by hyperviscosity blood, immune complex-mediated vascular damage, paraprotein deposition, amyloid deposition. Specific skin occur rarely generally consist translucent,...