A5038385206- Redox biology and oxidative stress
- Sulfur Compounds in Biology
- Microbial Community Ecology and Physiology
- Air Quality and Health Impacts
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Genomics and Phylogenetic Studies
- Microbial Metabolic Engineering and Bioproduction
- Advanced Glycation End Products research
- Eicosanoids and Hypertension Pharmacology
- Microbial bioremediation and biosurfactants
- Climate Change and Health Impacts
- bioluminescence and chemiluminescence research
- Genomics, phytochemicals, and oxidative stress
- S100 Proteins and Annexins
- Microbial metabolism and enzyme function
- Pulmonary Hypertension Research and Treatments
- Glutathione Transferases and Polymorphisms
- Coastal wetland ecosystem dynamics
- Tracheal and airway disorders
- Fatty Acid Research and Health
- Sarcoidosis and Beryllium Toxicity Research
- Eosinophilic Esophagitis
- Protease and Inhibitor Mechanisms
- Advanced Fluorescence Microscopy Techniques
University of North Carolina at Chapel Hill
2015-2025
University of North Carolina Health Care
2025
University of Vermont
2020-2024
Cornell University
2017
The bacterial strain TR3.2T was isolated from aerobic bioreactor-treated soil a polycyclic aromatic hydrocarbon (PAH)-contaminated site in Salisbury, NC, USA. Strain identified as member of 'Pyrene Group 2' or 'PG2', previously uncultivated cluster organisms associated with the degradation high-molecular-weight PAHs by stable-isotope probing. Based on its 16S rRNA gene sequence, classified class Gammaproteobacteria but possessed only 90.5 % identity to closest described relative,...
A bacterial strain designated Ca6T was isolated from polycyclic aromatic hydrocarbon (PAH)-contaminated soil the site of a former manufactured gas plant in Charlotte, NC, USA, and linked phylogenetically to family Rhodocyclaceae class Betaproteobacteria. Its 16S rRNA gene sequence highly similar globally distributed environmental sequences, including those previously 'Pyrene Group 1' demonstrated grow on PAHs phenanthrene pyrene by stable-isotope probing. The most closely related described...
Abstract Protein-S-glutathionylation is a post-translational modification involving the conjugation of glutathione to protein thiols, which can modulate activity and structure key cellular proteins. Glutaredoxins (GLRX) are oxidoreductases that regulate this process by performing deglutathionylation. However, GLRX has five cysteines potentially vulnerable oxidative modification, associated with aggregation loss activity. To date, oxidatively modified their relative susceptibilities remain...
Oxidative stress is a commonly cited mechanism of toxicity environmental agents. Ubiquitous chemicals such as the diesel exhaust component 1,2-naphthoquinone (1,2-NQ) induce oxidative by redox cycling, which generates hydrogen peroxide (H2O2). Cysteinyl thiolate residues on regulatory proteins are subjected to modification H2O2 in physiological contexts and also toxicological targets oxidant induced contaminants. We investigated whether exposure environmentally relevant concentrations 1,2-NQ...
Changes in the oxidative (redox) environment accompany idiopathic pulmonary fibrosis (IPF). S-glutathionylation of reactive protein cysteines is a post-translational event that transduces oxidant signals into biological responses. We recently demonstrated increases promote fibrosis, which was mitigated by deglutathionylating enzyme glutaredoxin (GLRX). However, targets fibrogenesis remain unknown. In present study we addressed whether extracellular matrix target for S-glutathionylation....
While oxidative stress is a commonly cited toxicological mechanism, conventional methods to study it suffer from number of shortcomings, including destruction the sample, introduction potential artifacts, and lack specificity for reactive species involved. Thus, there current need in field toxicology non-destructive, sensitive, specific that can be used observe quantify intracellular redox perturbations, more referred as stress. Here, we present method use two genetically-encoded fluorogenic...
While oxidative stress is a commonly cited toxicological mechanism, conventional methods to study it suffer from number of shortcomings, including destruction the sample, introduction potential artifacts, and lack specificity for reactive species involved. Thus, there current need in field toxicology non-destructive, sensitive, specific that can be used observe quantify intracellular redox perturbations, more referred as stress. Here, we present method use two genetically-encoded fluorogenic...