A5054929012- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- DNA and Nucleic Acid Chemistry
- Antimicrobial Peptides and Activities
- Monoclonal and Polyclonal Antibodies Research
- Metal-Organic Frameworks: Synthesis and Applications
- Molecular Junctions and Nanostructures
- RNA Interference and Gene Delivery
- Biochemical and Structural Characterization
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- Ferrocene Chemistry and Applications
- Carbohydrate Chemistry and Synthesis
- Bacteriophages and microbial interactions
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- Force Microscopy Techniques and Applications
- X-ray Diffraction in Crystallography
- Cannabis and Cannabinoid Research
- Nanopore and Nanochannel Transport Studies
- Crystallization and Solubility Studies
- Photochromic and Fluorescence Chemistry
- Innovative Microfluidic and Catalytic Techniques Innovation
- Machine Learning in Materials Science
Wageningen University & Research
2016-2025
Royal Society of Chemistry
2024
Centre National pour la Recherche Scientifique et Technique (CNRST)
2024
Ruhr University Bochum
2012-2020
Hebrew University of Jerusalem
2014-2018
Faculty (United Kingdom)
2014-2016
Jerusalem College of Technology
2014-2016
Center for NanoScience
2014-2015
Utrecht University
2002-2012
Pharmo Institute
2011
Significance Multidrug-resistant bacteria present an acute problem to medicine, generating interest in novel antimicrobial strategies. Antimicrobial peptides currently are being investigated, both as antibiotics and immunomodulatory agents. Many interact with the bacterial membrane, a previously underexplored antibiotic target. We system-based study of mode action small cationic mechanisms that use defend against them. show peptide integration into membrane causes delocalization essential...
A series of defect-engineered metal–organic frameworks (DEMOFs) derived from parent microporous MOFs was obtained by systematic doping with defective linkers during synthesis, leading to the simultaneous and controllable modification coordinatively unsaturated metal sites (CUS) introduction functionalized mesopores. These materials were investigated via temperature-dependent adsorption/desorption CO monitored FTIR spectroscopy under ultra-high-vacuum conditions. Accurate structural models...
Abstract A mixed‐linker solid‐solution approach was employed to modify the metal sites and introduce structural defects into mixed‐valence Ru II/III analogue of well‐known MOF family [M 3 II,II (btc) 2 ] (M=Cu, Mo, Cr, Ni, Zn; btc=benzene‐1,3,5‐tricarboxylate), with partly missing carboxylate ligators at paddle‐wheels. Incorporation pyridine‐3,5‐dicarboxylate (pydc), which is same size as btc but carries lower charge, a second, defective linker has led isoreticular derivatives Ru‐MOF,...
A novel concept to improve the catalytic functions of nucleic acids (DNAzymes) is introduced. The method involves conjugation a DNA recognition sequence (aptamer) DNAzyme, yielding hybrid structure termed “nucleoapzyme”. Concentrating substrate within “nucleoapzyme” leads enhanced activity, displaying saturation kinetics. Different modes aptamer/DNAzyme units and availability different aptamer sequences for provide diverse means design improved catalysts. This exemplified with (i)...
ConspectusResistance of pathogenic bacteria against currently marketed antibiotics is again increasing. To meet the societal need for effective cures, scientists are faced with challenge developing more potent but equally bacteria-specific drugs. Currently, most efforts directed toward modification existing antibiotics, ideally, compounds a new mode action required. In this Account, we detail our findings in area novel metal-based antibiotics. Our strategy based on simple antimicrobial...
Significance Spatial variations in microviscosity are triggered throughout plant cells, and these provide insight into local mechanobiological processes. However, it has so far been challenging to visualize such living cells. Here we report an imaging toolbox of chemically modified molecular rotors that yield complete maps several key cell structures. This opens up new ways understand the role mechanical stress regulation biological
Luminescent nucleic acid-stabilized Ag nanoclusters (Ag NCs) are applied for the optical detection of DNA and multiplexed analysis genes. Two different sensing modules including NCs as luminescence labels described. One module involves assembly a three-component composed NC quencher-modified acid hybridized with scaffold that is complementary to target DNA. The quenched in nanostructure. strand displacement by separates acid-functionalized NCs, leading turned-on readout process. By...
Mersacidin, gallidermin, and nisin are lantibiotics, antimicrobial peptides containing lanthionine. They show potent antibacterial activity. All three interfere with cell wall biosynthesis by binding lipid II, but they display different levels of interaction the cytoplasmic membrane. On one end spectrum, mersacidin interferes II without integrating into bacterial membranes. other readily integrates membranes, where it forms large pores. It destroys membrane potential causes leakage nutrients...
Genetically encoded tyrosine (Y-tag) can be utilized as a latent anchor for inducible and site-selective conjugation. Upon oxidation of with mushroom tyrosinase, strain-promoted cycloaddition (SPOCQ) the resulting 1,2-quinone various bicyclo[6.1.0]nonyne (BCN) derivatives led to efficient The method was applied fluorophore labeling laminarinase A site-specific preparation an antibody–drug conjugate.
The attachment of lipids to C- or N-terminally positioned lysine side-chain amino groups increases the activity a short synthetic (Arg-Trp)3 antimicrobial peptide significantly, making these peptides even active against pathogenic Gram-negative bacteria. Thus, with strong S. aureus (1.1-2 μM) and good A. baumannii P. aeruginosa (9-18 was identified. most promising causes 50% hemolysis at 285 μM shows some selectivity human cancer cell lines. Interestingly, increased ferrocenoylated is mostly...
We designed supramolecular nucleoapzyme systems displaying enhanced dopamine-oxidizing abilities using computational simulations, and developed a system having switchable catalytic activities.
A series of small synthetic arginine and tryptophan containing peptides was prepared analyzed for their antibacterial activity. The effect N-terminal substitution with metallocenoyl groups such as ferrocene (FcCO) ruthenocene (RcCO) investigated. Antibacterial activity in different media, growth inhibition, killing kinetics the most active were determined. toxicity selected derivatives determined against erythrocytes three human cancer cell lines. It shown that replacement an residue a...
A concept to tailor catalytic nucleic acid structures is introduced. The method involves the covalent conjugation of catalytically active metal ion complexes sequence-specific ligand-binding acids (aptamers) yielding hybrids termed "nucleoapzymes" that act as enzyme-mimicking based structures. concentration substrate by aptamer binding site, in close proximity complex models site native enzymes and yields systems. possibility tether sites 3′- or 5′-ends aptamer, internal bases associated...
Microscale thermophoresis (MST) is used to follow the dissociation constants corresponding ATTO 488-labeled adenosine triphosphate (ATP) and ATP-aptamer or mutants that include two binding sites for ATP ligand. A set of eight mutants, where thymidine bases, within reported aptamer sites, are substituted with cytosine examined. The MST-derived constant Kd = 31 ± 3 μM, whereas most show lower affinity (higher values) toward One mutant reveals, however, a higher ligand, as compared ATP-aptamer....
New antibiotics are urgently needed to address the mounting resistance challenge. In early drug discovery, one of bottlenecks is elucidation targets and mechanisms. To accelerate antibiotic research, we provide a proteomic approach for rapid classification compounds into those with precedented unprecedented modes action. We established response library Bacillus subtilis covering 91 comparator compounds, mathematical was developed aid data analysis. Comparison responses (CoPR) allows...
Peptide hormones are decorated with post-translational modifications (PTMs) that crucial for receptor recognition. Tyrosine sulfation on plant peptide is, example, essential growth and development. Measuring the occurrence position of sulfotyrosine however, compromised by major technical challenges during isolation detection. Nanopores can sensitively detect protein PTMs at single-molecule level. By translocating PTM variants pentapeptide hormone phytosulfokine (PSK) through a nanopore, we...
By taking a systematic approach several short organometallic AMP conjugates were discovered which have very low hemolytic activity but virtually the same antimicrobial against MRSA as that of vancomycin.