Early confirmation of SARS-CoV-2 is a key point in the timely management infected patients and contact persons. Routine diagnostics COVID-19 cases relies on RT-PCR detection two or three unique sequences virus. A serious problem for laboratories how to interpret inconclusive samples which are positive only one specific genes.A total 16364 naso-oropharyngeal swabs were collected tested with Real-TM kit (Sacace Biotechnologies, Italy) between May September 2020. We retrospectively analyzed...

: Intestinal colonization by multidrug-resistant (MDR) bacteria is considered one of the main risk factors for invasive infections in hematopoietic stem-cell transplant (HSCT) setting, associated with hard-to-eradicate microorganisms. The aim this study was to assess rate intestinal MDR and their microbial spectrum a group post-HSCT patients genetic determinants beta-lactam glycopeptide resistance recovered isolates, as well determine epidemiological relation between them.

Mucormycosis is a relatively rare infection but with high mortality rate due to the difficult and time-consuming diagnostic therapeutic process. The authors present first case of rhino-orbital-cerebral mucormycosis, histologically microbiologically proven, in patient after COVID-19 Bulgaria.

Many studies report an increase in antimicrobial resistance of Gram - negative bacteria during the COVID-19 pandemic. Our aim was to evaluate epidemiological relationship between carbapenem-resistant (CR) Enterobacteriaceae isolates from patients wards and investigate main mechanisms carbapenem these period April 2020-July 2021. A total 45 were studied: Klebsiella pneumoniae (n = 37), oxytoca 2), Enterobacter cloacae complex 4) Escherichia coli 2). Multiplex PCR used for detection genes...

Mucormycosis, caused by the widespread molds of Mucorales order, is an insidious infection that manifests in different clinical forms. Even most benign form, cutaneous mucormycosis, can present with severe complications and a fatal outcome patients suppressed immune system underlining comorbidities. We rare case proven primary multifocal mucormycosis child newly diagnosed acute leukemia without multiorgan dissemination. Various laboratory techniques (histopathological, cultural...

The aim of the study was to evaluate concordance rapid antimicrobial susceptibility testing (AST) results obtained by HB&L Uroquattro instrument performed directly from positive urine sam¬ples in comparison with standard Kirby-Bauer disk-diffusion method isolated colonies. We included 122 samples defined as system screening method. AST them instrument. Simultaneously, all were cultured on CLED, MacConkey and Blood agar incubated for 24h at 37°C. Species identification pathogens solid...

The aim of this study is to evaluate the antimicrobial susceptibility invasive isolates Serratia marcescens, associated with blood stream infections (BSIs) in patients hospitalized Varna University Hospital, Bulgaria, as well identify genetic mechanisms responsible for 3rd generation cephalosporin and carbapenem-resistance among these isolates. A total 45 consecutive S. marcescens isolates, obtained from cultures BSIs, during an 8-year period (2016-2023) were included. Species identification...

Abstract Aim the aim of this study is to evaluate accuracy HB&L Uroquattro instrument (Alifax, Italy) and Residual Antimicrobial Activity test (RAA) for rapid correct diagnosis Urinary Tract Infections (UTIs) compare results with those obtained classical cultural method. Materials methods A total 1600 urine samples, collected prospectively from 842 ambulatory patients in Varna city, Bulgaria, were included study. All samples tested bacterial growth RAA by Italy). Simultaneously, each...

: The aim of the current report is to present three cases necrotizing fasciitis and sepsis caused by

Rhinocerebral mucormycosis is a rare and rapidly progressive fungal infection caused by opportunist fungi from the Mucoraceae family, which are omnipresent in nature. Fungal sporangiospores discharged into atmosphere could be inhaled or directly penetrate damaged skin mucosa, they strongly prefer angioinvasive spread. These relatively opportunistic infections have spiked recent years, particularly during coronavirus disease identified 2019 (COVID-19). Herein, we describe series of three...

Introduction : The human body is colonized by a large number of different bacterial and fungal species, with most microorganisms inhabiting the skin, oral cavity, lower parts gastrointestinal tract. Aim: aim this study was to investigate species diversity clinically significant Staphylococcus spp. isolates, obtained from blood cultures patients central venous catheter (CVC) following hematopoietic stem cell transplantation (HSCT) during period January 2019–December 2020, as well test their...

The SARS-CoV-2 pandemic is the most devastating health crisis our generation has seen. present study tries to gather more epidemiological data and improve knowledge of demographic factors responsible for a higher incidence COVID-19. We analyzed real time polymerase chain reaction (RT-PCR) results obtained in one biggest tertiary hospitals Bulgaria during first year COVID-19 (May 2020–April 2021). For this period, almost 40% all tested samples hospitalized patients care workers University...

first_page settings Order Article Reprints Font Type: Arial Georgia Verdana Size: Aa Line Spacing:  Column Width:  Background: Open AccessAbstract In Vitro Activity of Ceftazidime-Avibactam against Gram—Negative Bacteria Recovered from Blood and Fecal Samples Patients after Hematopoietic Stem-Cell Transplantation † by Denis Niyazi 1,2,*, Ilina Micheva 1,3, Dobromira Savova 1,2 Temenuga Stoeva 1 Department Microbiology Virology, Faculty Medicine, Medical University Varna, 9000 Bulgaria 2...

Patients after hematopoietic stem cell transplantation are a risk group for developing various complications. Most of them caused by infectious agents. Important in the post-transplant period is prevention and therapy these complications, which often lead to high morbidity mortality rates. The aim this review present briefly options bacterial mycotic complications patients following transplantation.

The Article Abstract is not available.

The recipients of hematopoietic stem cell transplantation (HSCT) tend to develop invasive infections because the immunosuppression caused by underlying disease or itself. gastro¬intestinal tract is major source causative agents, often demonstrating multidrug resistance. aim this study investigate in vitro activity ceftazidime-avibactam (CZA) against 3rd generation ceph¬alosporin and/or carbapenem resistant (CPR) Gram-negative bacteria obtained from blood and fecal sam¬ples patients following...

Abstract Rapid point-of-care tests that detect antigens specific for SARS-CoV-2 are less expensive and faster than the standard molecular methods. However, their accuracy varies widely between tests, populations settings. We decided to assess diagnostic precision of antigen in a real-life hospital environment. In cohort 2677 hospitalized patients, we found sensitivity 60.2% (95% CI 57.1%-63.4%) specificity 98.1% 97.4%-98.7%) when compared RT-PCR. The testing could be acceptable setting,...

Invasive fungal infections are common complication in hematopoietic stem cell transplant recipients, often leading to high morbidity and mortality rates. Furthermore, when invasive co-infections diagnosed the prognosis is rarely favorable. Here, we present a rare case of 47-year-old HIV-negative male with pulmonary co-infection caused by Aspergillus sp. Pneumocystis jirovecii, complicated Cytomegalovirus reactivation following second allogeneic transplantation fatal outcome. 2012 Elsevier...

Serratia marcescens is an important member of the order Enterobacterales, even though it was initially considered to be slightly virulent. Today indisputable fact that opportunistic pathogen responsible for a wide range nosocomial infections. The main risk group immunocompromised patients, both adults and newborns, patients after different medical interventions such as bronchoscopy, central venous catheterization, urinal catheters, surgical drainage, tracheostomy, mechanical ventilation....

Antimicrobial resistance is a pandemic which affects the therapeutic options of many people in need by rendering most known antibacterial agents ineffective. The answer to problem may be found old books written ancient philosophers who used various plants treat sick. Our objective was study properties commonly plant, Tagetes erecta L., and determine minimum inhibitory concentration (MIC) bactericidal (MBC) crude ethanol extract. A maceration protocol obtain extract dried aerial parts T....

Individuals undergoing hematopoietic stem cell transplantation are a peculiar group of patients in whom the risk developing infectious complications is very high. Various groups antimicrobials used for prophylaxis and therapy, but growing resistance among bacterial isolates about to derail all attempts prevent severe complications. The aim this review briefly present different types multidrug-resistant bacteria causing infections these patients, as well introduce most common mechanisms...