A5060870665- Microbial Natural Products and Biosynthesis
- Carbohydrate Chemistry and Synthesis
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Synthetic Organic Chemistry Methods
- Sexual function and dysfunction studies
- Hormonal and reproductive studies
- Urinary Bladder and Prostate Research
- Sexual Differentiation and Disorders
- Urological Disorders and Treatments
- Photosynthetic Processes and Mechanisms
- Enzyme Catalysis and Immobilization
- Bladder and Urothelial Cancer Treatments
- Pelvic floor disorders treatments
- Prostate Cancer Diagnosis and Treatment
- Genetic and Kidney Cyst Diseases
- Protist diversity and phylogeny
- Fungal and yeast genetics research
- Sperm and Testicular Function
- Lysosomal Storage Disorders Research
- Reproductive System and Pregnancy
- Microbial Metabolism and Applications
- Erythrocyte Function and Pathophysiology
- Microtubule and mitosis dynamics
- Genital Health and Disease
University of Bristol
2018-2024
New York Proton Center
2014-2016
Beatson West of Scotland Cancer Centre
2012
Western Infirmary
2012
Living biological systems rely on the continuous operation of chemical reaction networks. These networks sustain out-of-equilibrium regimes in which energy is continually converted into controlled mechanical work and motion. Out-of-equilibrium have also enabled design successful development artificial autonomously operating molecular machines, comprising pairs formally—but non-microscopically—reverse pathways drive motion at level. In systems, concurrent multiple by chemoselectivity enzymes...
Abstract Mupirocin is a clinically important antibiotic produced by trans ‐AT Type I polyketide synthase (PKS) in Pseudomonas fluorescens . The major bioactive metabolite, pseudomonic acid A (PA−A), assembled on tetrasubstituted tetrahydropyran (THP) core incorporating 6‐hydroxy group proposed to be introduced α‐hydroxylation of the thioester acyl carrier protein (ACP) bound chain. Herein, we describe an vitro approach combining purified enzyme components, chemical synthesis, isotopic...
Abstract The delineation of the complex biosynthesis potent antibiotic mupirocin, which consists a mixture pseudomonic acids (PAs) isolated from Pseudomonas fluorescens NCIMB 10586, presents significant challenges, and timing mechanisms several key transformations remain elusive. Particularly intriguing are steps that process linear backbone initial polyketide assembly phase to generate first cyclic intermediate PA‐B. These include epoxidation as well incorporation tetrahydropyran (THP) ring...
Mupirocin is a clinically important antibiotic produced by Pseudomonas fluorescens NCIMB 10586 that assembled complex trans-AT polyketide synthase. The fragment, monic acid, esterified 9-hydroxynonanoic acid (9HN) side chain which essential for biological activity. ester assembly initialised from 3-hydroxypropionate (3HP) starter unit attached to the acyl carrier protein (ACP) MacpD, but fate of this species unknown. Herein we report application NMR spectroscopy, mass spectrometry, chemical...
Toxin-antitoxin (TA) systems are present in many bacteria and play important roles bacterial growth, physiology, pathogenicity. Those that best studied the type II TA systems, which both toxins antitoxins proteins. The HicAB system is one of prototypic found species. Complex interactions between protein toxin (HicA), antitoxin (HicB), DNA upstream encoding genes regulate activity this system, but few structural details available about how HicA destabilizes HicB-DNA complex. Here, we...
Mupirocin, a commercially available antibiotic produced by Pseudomonas fluorescens NCIMB 10586, and thiomarinol, isolated from the marine bacterium Pseudoalteromonas sp. SANK 73390, both consist of polyketide-derived monic acid homologue esterified with either 9-hydroxynonanoic (mupirocin, 9HN) or 8-hydroxyoctanoic (thiomarinol, 8HO). The mechanisms formation these deceptively simple 9HN 8HO fatty moieties in mup tml, respectively, remain unresolved. To define starter unit generation,...
The leinamycin family of polyketides are promising antitumour antibiotics, yet several aspects their biosynthesis remain elusive. All members bear a sulfur‐containing moiety which is essential for the anticancer activity exhibited by leinamycin. key building blocks required incorporation these functionalities introduced in final module polyketide synthase (PKS), elegantly combines β‐branching and thiocysteine to generate diverse library sulfur‐based molecular scaffolds. Two acyl carrier...
Menisporopsin A is a fungal bioactive macrocyclic polylactone, the biosynthesis of which requires only reducing (R) and nonreducing (NR) polyketide synthases (PKSs) to guide series esterification cyclolactonization reactions. There no structural information pertaining these PKSs. Here, we report solution characterization singlet doublet acyl carrier protein (ACP 2 ACP 1 ‐ACP )–thioesterase (TE) domains from NR‐PKS involved in menisporopsin biosynthesis. Small‐angle X‐ray scattering (SAXS)...
The acyl carrier protein (ACP) is an indispensable component of both fatty acid and polyketide synthases primarily responsible for delivering intermediates to enzymatic partners. At present, increasing numbers multidomain ACPs have been discovered with roles in molecular recognition trans-acting partners as well metabolic flux. Further structural information required provide insight into their function, yet date, the only high-resolution structure this class be determined that doublet ACP...
You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Medical and Non-surgical1 Apr 2014MP48-04 PREDICTORS OF POOR RESPONSE TO TRANSDERMAL TESTOSTERONE THERAPY IN MEN WITH METABOLIC SYNDROME Ashley Winter, Anthony Marte, Morgan Kelly, Michael Funaro, Peter Schlegel, Darius Paduch WinterAshley Winter More articles by this author , MarteAnthony Marte KellyMorgan Kelly FunaroMichael Funaro SchlegelPeter Schlegel PaduchDarius View All Author...
Abstract The delineation of the complex biosynthesis potent antibiotic mupirocin, which consists a mixture pseudomonic acids (PAs) isolated from Pseudomonas fluorescens NCIMB 10586, presents significant challenges, and timing mechanisms several key transformations remain elusive. Particularly intriguing are steps that process linear backbone initial polyketide assembly phase to generate first cyclic intermediate PA‐B. These include epoxidation as well incorporation tetrahydropyran (THP) ring...
The leinamycin family of polyketides are promising antitumour antibiotics, yet several aspects their biosynthesis remain elusive. All members bear a sulfur‐containing moiety which is essential for the anticancer activity exhibited by leinamycin. key building blocks required incorporation these functionalities introduced in final module polyketide synthase (PKS), elegantly combines β‐branching and thiocysteine to generate diverse library sulfur‐based molecular scaffolds. Two acyl carrier...
You have accessJournal of UrologyKidney Cancer: Localized IV1 Apr 2014MP59-03 RENAL TISSUE ABLATION PRODUCED WITH WST-11 VASCULAR TARGETED PHOTODYNAMIC THERAPY: SAFETY ASSESSMENT, HISTOPATHOLOGIC AND RADIOGRAPHIC ANALYSIS Simon Kimm, Tatum Tarin, Thomas Wimmer, Srinivasaraghavan Srimathveeravalli, Ashley Winter, Jeremy Durack, Stephen Solomon, Avigdor Scherz, Peter Scardino, and Jonathan Coleman KimmSimon Kimm More articles by this author , TarinTatum Tarin WimmerThomas Wimmer...
You have accessJournal of UrologyGeneral & Epidemiological Trends Socioeconomics: Practice Patterns, Cost Effectiveness I1 Apr 2015MP16-02 INFLUENCE OF INTERDEPARTMENTAL COLLABORATION ON UTILIZATION NEOADJUVANT CHEMOTHERAPY PRIOR TO CYSTECTOMY; A 14-YEAR EXPERIENCE Ashley Winter, Bashir Awamleh, Sameer Mittal, Shiyi Jin, Daniel Nguyen, Scott Tagawa, and Douglas Scherr WinterAshley Winter More articles by this author , AwamlehBashir Awamleh MittalSameer Mittal JinShiyi Jin NguyenDaniel Nguyen...
You have accessJournal of UrologySexual Function/Dysfunction/Andrology: Medical and Non-Surgical Therapy II1 Apr 2015PD45-08 COMPARISON OF CLOMIPHENE CITRATE AND TRANSDERMAL TESTOSTERONE REPLACEMENT THERAPY IN THEIR INFLUENCE ON HORMONAL METABOLIC CHANGES THE TREATMENT HYPOGONADISM Daniel Lee, Adrien Bernstein, Alex Sarkisian, Matthew Wosnitzer, Ashley Winter, Darius Paduch LeeDaniel Lee More articles by this author , BernsteinAdrien Bernstein SarkisianAlex Sarkisian WosnitzerMatthew...
You have accessJournal of UrologyInfertility: Basic Research, Physiology, Pathophysiology1 Apr 2015MP76-19 DNA FRAGMENTATION MAPPING OF THE MALE GENITAL TRACT Ashley Winter, Bobby Najari, Gianpiero Palermo, Darius Paduch, and Marc Goldstein WinterAshley Winter More articles by this author , NajariBobby Najari PalermoGianpiero Palermo PaduchDarius Paduch GoldsteinMarc View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.2935AboutPDF ToolsAdd to favoritesDownload CitationsTrack...
You have accessJournal of UrologyBladder Cancer: Natural History & Pathophysiology1 Apr 2014MP50-14 ASSOCIATION OF LYMPHOVASCULAR INVASION AT TRANSURETHRAL RESECTION BLADDER TUMOR WITH LONG TERM OUTCOMES IN PATIENTS UNDERGOING RADICAL CYSTECTOMY Ashley Winter, Daniel Lee, Bashir Awamleh, Lily Wang, David Green, and Douglas Scherr WinterAshley Winter More articles by this author , LeeDaniel Lee AwamlehBashir Awamleh WangLily Wang GreenDavid Green ScherrDouglas View All Author...
You have accessJournal of UrologyProstate Cancer: Localized III1 Apr 2015MP56-20 HYPOGONADISM AND VARICOCELE STATUS AS RISK FACTORS FOR ADVERSE PATHOLOGIC FEATURES AT RADICAL PROSTATECTOMY Bobby Najari, Ashley Winter, Matthew Katz, Douglas Scherr, and Marc Goldstein NajariBobby Najari More articles by this author , WinterAshley Winter KatzMatthew Katz ScherrDouglas Scherr GoldsteinMarc View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.2084AboutPDF ToolsAdd to...
You have accessJournal of UrologyGeneral & Epidemiological Trends Socioeconomics: Practice Patterns, Cost Effectiveness I1 Apr 2015MP16-03 STRATEGIC IMPROVEMENT IN PROSTATE BIOPSY FIXATION; ROLE OF INTERDISCIPLINARY QUALITY COLLABORATION UROLOGIC PRACTICE Ashley Winter, Paul DiMaggio, and Brian Robinson WinterAshley Winter More articles by this author , DiMaggioPaul DiMaggio RobinsonBrian View All Author Informationhttps://doi.org/10.1016/j.juro.2015.02.810AboutPDF ToolsAdd to...
You have accessJournal of UrologyImaging/Radiology III1 Apr 2014MP12-14 VASCULAR TARGETED PHOTODYNAMIC THERAPY IN THE SWINE URETER: TRANSMURAL ABLATION DOES NOT PRODUCE OBSTRUCTION SUBACUTE SETTING Ashley Winter, Simon Kimm, Haruyuki Takaki, Sebastien Monette, Avigdor Scherz, and Jonathan Coleman WinterAshley Winter More articles by this author , KimmSimon Kimm TakakiHaruyuki Takaki MonetteSebastien Monette ScherzAvigdor Scherz ColemanJonathan View All Author...
You have accessJournal of UrologyProstate Cancer: Markers I1 Apr 2016MP02-13 MICRORNA EXPRESSION PROFILING OF PROSTATE TISSUE SUGGESTS MIR-509-3P AS A DIAGNOSTIC MARKER FOR PROSTATIC ADENOCARCINOMA Jennifer Reifsnyder, Ashley Winter, Anna Mielnik, Sameer Mittal, Brian Robinson, Peter Schlegel, and Darius Paduch ReifsnyderJennifer Reifsnyder More articles by this author , WinterAshley Winter MielnikAnna Mielnik MittalSameer Mittal RobinsonBrian Robinson SchlegelPeter Schlegel PaduchDarius...
Abstract Introduction: oncosurgical safety of therapeutic mammaplasty (TM) is widely investigated. The interval between surgery and delivery adjuvant chemotherapy an integral part overall oncological safety. Therefore, we examined the time TM AC, compared it to wide local excision (WLE) mastectomy (Mx) with or without immediate breast reconstruction (IBR), respectively. Methods: data 174 patients who underwent TM, WLE Mx±IBR was analyzed retrospectively. All were operated within three units...