A5067876663- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Telomeres, Telomerase, and Senescence
- Genomics and Chromatin Dynamics
- Cancer Genomics and Diagnostics
- Ubiquitin and proteasome pathways
- DNA and Nucleic Acid Chemistry
- Advanced biosensing and bioanalysis techniques
- Genomic variations and chromosomal abnormalities
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- CAR-T cell therapy research
- Endoplasmic Reticulum Stress and Disease
- Bacterial Genetics and Biotechnology
- Peptidase Inhibition and Analysis
- PARP inhibition in cancer therapy
- Plant nutrient uptake and metabolism
- Mesenchymal stem cell research
- Fungal and yeast genetics research
- Molecular Biology Techniques and Applications
- Genetic and Kidney Cyst Diseases
Nankai University
2024
University of Oklahoma Health Sciences Center
2024
OU Health
2024
University of Copenhagen
2013-2023
Nanjing University of Chinese Medicine
2022
Weifang Medical University
2022
AstraZeneca (Sweden)
2016
University of Oxford
2014
Institute of High Energy Physics
2012
Chinese Academy of Sciences
2012
Abstract PICH is a SNF2 family DNA translocase that binds to ultra-fine bridges (UFBs) in mitosis. Numerous roles for have been proposed from protein depletion experiments, but consensus has failed emerge. Here, we report deletion of avian cells causes chromosome structural abnormalities, and hypersensitivity an inhibitor Topoisomerase II (Topo II), ICRF-193. ICRF-193-treated −/− undergo sister chromatid non-disjunction anaphase, frequently abort cytokinesis. co-localizes with Topo IIα on...
In preparation for mitotic cell division, the nuclear DNA of human cells is compacted into individualized, X-shaped chromosomes1. This metamorphosis driven mainly by combined action condensins and topoisomerase IIα (TOP2A)2,3, has been observed using microscopy over a century. Nevertheless, very little known about structural organization chromosome. Here we introduce workflow to interrogate chromosomes based on optical trapping manipulation. allows high-resolution force measurements...
Abstract Oncogene activation creates DNA replication stress (RS) in cancer cells, which can generate under-replicated regions (UDRs) that persist until cells enter mitosis. UDRs also have the potential to bridges anaphase or micronuclei daughter could promote genomic instability. To suppress such damaging changes genome, human developed a strategy conduct ‘unscheduled’ synthesis mitosis (termed MiDAS) serves rescue loci. Previous studies shown MiDAS proceeds via POLD3-dependent pathway shows...
Efficient repair of DNA double strand breaks and interstrand cross-links requires the homologous recombination (HR) pathway, a potentially error-free process that utilizes sequence as template. A key player in HR is RAD51, eukaryotic ortholog bacterial RecA protein. RAD51 can polymerize on to form nucleoprotein filament facilitates both search for sequences subsequent invasion required initiate HR. Because its pivotal role HR, subject numerous positive negative regulatory influences. Using...
Bloom's syndrome helicase (BLM) is a member of the RecQ family DNA helicases, which play key roles in maintenance genome integrity all organism groups. We describe crystal structures BLM domain complex with and an antibody fragment, as well SAXS association studies solution. show unexpected nucleotide-dependent interaction core conserved, poorly characterized HRDC domain. The BLM–DNA shows unusual base-flipping mechanism unique positioning duplex relative to domains. Comparison other...
// Özgün Özer 1 , Rahul Bhowmick Ying Liu and Ian D. Hickson Center for Chromosome Stability Healthy Aging, Department of Cellular Molecular Medicine, University Copenhagen, 2200 Denmark Correspondence to: Hickson, email: iandh@sund.ku.dk Keywords: alternative lengthening telomeres; common fragile sites; RAD52; MiDAS; MUS81; Received: November 02, 2017 Accepted: February 25, 2018 Published: March 23, 2018...
Common fragile sites (CFSs) are conserved genomic regions prone to break under conditions of replication stress (RS). Thus, CFSs hotspots for rearrangements in cancer and contribute its chromosomal instability. Here, we have performed a global analysis proteins that recruit upon mild RS identify novel players CFS stability. To this end, Chromatin Immunoprecipitation (ChIP) FANCD2, protein localizes specifically G2/M, coupled mass spectrometry acquire interactome. Our strategy was validated...
Significance Folate deficiency is associated with multiple disorders in humans. Through the analysis of fragile X syndrome locus ( FRAXA ) immortalized human lymphocytes or fibroblasts, we demonstrate that undergoes DNA synthesis mitosis (MiDAS). We this process occurs via break-induced replication and requires SLX1/SLX4 endonuclease complex, RAD51 recombinase POLD3, a subunit polymerase delta. also other loci undergo MiDAS upon folate stress. This study reveals function SLX1 maintenance...
Significance Dietary folate deficiency is associated with fetal neural tube defects, psychological disorders, and age-associated dementia. However, it remains unclear how could be a causative factor in such diverse range of disorders. Through analysis the FRAXA locus, which contains an extensive CGG repeat sequence, we show that deprivation triggers mitotic missegregation locus. Moreover, entire chromosome X becomes unstable during period long-term deprivation. Considering human genome...
Article14 February 2022Open Access Transparent process SCAI promotes error-free repair of DNA interstrand crosslinks via the Fanconi anemia pathway Lisa Schubert Protein Signaling Program, Novo Nordisk Foundation Center for Research, University Copenhagen, Denmark Contribution: Conceptualization, Data curation, Funding acquisition, Investigation, Methodology, Project administration Search more papers by this author Ivo A Hendriks orcid.org/0000-0002-1439-3701 Proteomics Methodology Emil P T...
Recent advances in targeted protein degradation (TPD) have propelled it to the forefront of small molecular drug discovery. Among these, hydrophobic tagging (HyT) strategies garnered significant interest. Carbon-based tags been recognized as effective Hyts for degrading a variety target proteins. In this study, we introduce novel class potential EGFR degraders first time, which combine Gefitinib with silicon-based (SiHyT). The most promising candidate, degrader 7, links simple TBDPS silyl...
PSY3 and CSM2 bind by X‐ray crystallography ( View interaction ) , Shu 1 2 physically interact x ray scattering