A5037167550- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- DNA and Nucleic Acid Chemistry
- Nanopore and Nanochannel Transport Studies
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Peptidase Inhibition and Analysis
Lodz University of Technology
2017-2024
Institute of Organic Chemistry
2017-2024
University of Warsaw
2024
Post-transcriptional chemical modifications of (t)RNA molecules are crucial in fundamental biological processes, such as translation. Despite their importance and accumulating evidence linking them to various human diseases, technical challenges have limited detection accurate quantification. Here, we present a sensitive capillary nanoflow liquid chromatography mass spectrometry (nLC-MS) pipeline for quantitative high-resolution analysis ribonucleoside from complex samples. We evaluated two...
Efficient preparation of ct<sup>6</sup>A-tRNA fragments a sequence native anticodon arms:<italic>S. pombe</italic>tRNA<sup>i</sup>(single ct<sup>6</sup>A modification) and<italic>E. coli</italic>tRNA<sup>Lys</sup>containing mnm<sup>5</sup>s<sup>2</sup>U, ψ and modifications.
Abstract The synthesis of the protected form 2‐methylthio‐ N 6 ‐threonylcarbamoyl adenosine ( ms 2 t A) was developed starting from or guanosine by using optimized carbamate method and, for first time, an isocyanate route. hypermodified nucleoside subsequently transformed into A ‐phosphoramidite monomer and used in a large‐scale precursor 17nt ‐oligonucleotide (the anticodon stem loop fragment tRNA Lys T. brucei ). Finally, stereochemically secure → ct cyclization at oligonucleotide level...
A new convenient method of ureido linkage formation in<bold>t6A</bold>/<bold>ms2t6A</bold>nucleosides greatly enhances the availability phosphoramidite monomers for chemical synthesis modified tRNA fragments.
The continuous deciphering of crucial biological roles RNA modifications and their involvement in various pathological conditions, together with key the use RNA-based therapeutics, has reignited interest studying occurrence identity non-canonical ribonucleoside structures during past years. Discovery structural elucidation new modified is usually achieved by combination liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) at nucleoside level stable isotope labeling...
ABSTRACT Circularization is at the frontier of therapeutic messenger RNA (mRNA) enhancements. Currently available enzymatic and ribozymatic methods for generating circular RNAs (circRNAs) face several challenges related to sequence limitations, purification, sub-optimal biological activity. The chemical circularization synthetic fragments potentially overcomes these limitations but applicable only extremely short sequences. Here, we report a novel approach accessing based on in vitro...
Abstract Invited for the cover of this issue is Elzbieta Sochacka and co‐workers at Lodz University Technology, Poland. The image depicted manual chemical synthesis tRNA fragment containing hypermodified cyclic 2‐methylthio‐N 6 ‐threonylcarbamoyladenosine (ms 2 ct A). Read full text article 10.1002/chem.201902411 .
A novel and efficient way for the synthesis of N6 -hydantoin-modified adenosines, which utilizes readily available -(N-Boc-α-aminoacyl)-adenosine derivatives, was developed. The procedure is based on epimerization-free, Tf2 O-mediated conversion Boc group into an isocyanate moiety, followed by intramolecular cyclization. Using this method two recently discovered hydantoin modified tRNA that is, cyclic -threonylcarbamoyl-adenosine (ct6 A) 2-methylthio-N6 -threonylcarbamoyladenosine (ms2 ct6...