A5003269898- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Toxoplasma gondii Research Studies
- Ion channel regulation and function
- Cardiomyopathy and Myosin Studies
- Reproductive tract infections research
- Cardiovascular Function and Risk Factors
- Lung Cancer Treatments and Mutations
- Parasitic Infections and Diagnostics
- Venomous Animal Envenomation and Studies
- Biochemical and Molecular Research
- Parasites and Host Interactions
- Monoclonal and Polyclonal Antibodies Research
- Ubiquitin and proteasome pathways
- Healthcare and Venom Research
- Insect and Pesticide Research
- Allergic Rhinitis and Sensitization
- Helminth infection and control
- Cancer Treatment and Pharmacology
- Toxin Mechanisms and Immunotoxins
- Traditional and Medicinal Uses of Annonaceae
- Plant Toxicity and Pharmacological Properties
- Marine Invertebrate Physiology and Ecology
- Cancer Immunotherapy and Biomarkers
- Cardiac Imaging and Diagnostics
Tecnológico de Monterrey
2025
University of Antofagasta
2015-2024
Millennium Institute on Immunology and Immunotherapy
2022-2024
Pontificia Universidad Católica de Chile
2024
Hospital Universitario Puerta de Hierro Majadahonda
2009-2023
Hospital Arnau de Vilanova
2022
Universidad de Colima
2011-2016
Massachusetts Institute of Technology
2016
Consejo Nacional de Investigaciones Científicas y Técnicas
2015
University of Birmingham
2014
It was shown in this work that the infectivity of metacyclic forms Trypanosoma cruzi affected upon interaction with monoclonal antibody (10D8), which reacts a carbohydrate epitope 35- and 50-kilodalton (kDa) surface glycoconjugates. The invasion Vero cells by strains Tulahuen G inhibited 50 to 67% presence 10D8 (10 micrograms/ml), whereas nonrelated Plasmodium berghei had no such effect. In mice were inoculated preincubated or passively received before challenge forms, considerable decrease...
Background Trichomonas vaginalis is the causative agent of human trichomoniasis, most common non-viral sexually transmitted infection world-wide. Despite its prevalence, little known about genetic diversity and population structure this haploid parasite due to lack appropriate tools. The development a panel microsatellite makers SNPs from mining parasite's genome sequence has paved way global analysis pathogen association with clinical phenotypes. Methodology/Principal Findings Here we...
A prominent feature of the life cycle intracellular parasites is profound morphological changes they undergo during development in vertebrate and invertebrate hosts. In eukaryotic cells, most cytoplasmic proteins are degraded proteasomes. Here, we show that transformation axenic medium trypomastigotes Trypanosoma cruzi into amastigote-like organisms, parasite from amastigotes trypomastigotes, prevented by lactacystin, or a peptide aldehyde inhibits proteasome function. Clasto-lactacystin, an...
Abstract Massive proliferations of scyphozoan jellyfish considerably affect human industries and irreversibly change food webs. Efforts to understand the role in marine ecosystems are based on a life cycle model described 200 years ago. According this paradigm pelagic medusae is considered seasonal alternates with benthic polyp stage from which it derives. However, we provide evidence that a) occurrence several species not restricted season year, they overwinter, b) polyp- medusa generations...
In this paper a method of using the "quick, easy, cheap, effective, rugged, and safe" (QuEChERS) extraction gas chromatography coupled to mass spectrometry detection (GC-MS) was developed for analysis five frequently applied pesticides in papaya avocado. The selected pesticides, ametryn, atrazine, carbaryl, carbofuran, methyl parathion, represent most commonly used classes (carbamates, organophosphorous, triazines). Optimum separation achieved all < 6.5 minutes. Validation avocado samples...
Summary We have previously shown that monoclonal antibodies directed to the 90 kDa glycoprotein and 35/50 glycoconjugate, present on surface of Trypansoma cruzi metacyclic trypomastigotes, inhibited host cell invasion. Here we investigated whether these molecules could be ligands for target receptor. Binding assays were performed by incubating Vero cells with sonicated parasite extract. Detection bound components was carried out using (MoAbs) 1G7 10D8, which recognize antigens respectively....
Here, we document for the first time presence of 26S proteasome and ubiquitin pathway in a protozoan parasite that is an early branch eukaryotic lineage. The Trypanosoma cruzi epimastigotes was identified as high molecular weight complex (1400 kDa) with ATP-dependent chymotrypsin-like activity against substrate Suc-LLVY-Amc. This inhibited by inhibitors showed same electrophorectic migration pattern yeast nondenaturating gels. About 30 proteins range 25−110 kDa were detected purified T....
Artemisia copa Phil. (Asteraceae) (known as copa-copa) is a native species of Chile used an infusion in traditional medicine by Atacameños people the Altiplano, highlands northern Chile. In this research, we have investigated for first time cholinesterase inhibition potential against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), chemical profiling infusions prepared from aerial parts A. high resolution spectrometry. addition, total phenolic, flavonoid content, antioxidant...
Kidney biopsies of patients with diabetic nephropathy (DN) and normal kidney function may exhibit interstitial fibrosis (IF) without reduction glomerular filtration rate (GFR) because hyperfiltration. The aim our study was to analyse the performance a set biomarkers tubular injury estimate extent IF in DN function.This cross-sectional included 118 adults diagnosed by biopsy GFR ≥90 mL/min/1.73 m2 control group healthy subjects. We measured urinary excretion monocyte chemoattractant protein-1...
Abstract Toxin phospholipases‐D present in the venom of Loxosceles spiders is principal responsible for local and systemic effects observed loxoscelism. In this study, we describe cloning, expression, functional evaluation, potential biotechnological application cDNAs, which code two new phospholipase D isoforms, LIPLD1 LIPLD2, spider laeta . The recombinant protein rLIPLD1 had hydrolytic activity on sphingomyelin vitro hemolytic human red blood cells, whereas rLIPLD2 was inactive. purified...
Proteases play an important role in the pathogenic mechanisms and differentiation events of protozoan parasites; proteasome/ubiquitin system is essential for maintaining state many cell types. A single input specific inhibitor proteasomes, lactacystin, prevented encystation parasite Entameoba invadens , whereas a cysteine protease inhibitor, E64, only delayed encystation. The ameba target lactacystin was purified it displayed features typical eukaryotic 20S proteasome complexes. In addition,...
To improve the analysis of pesticides in complex food matrices with economic importance, alternative chromatographic techniques, such as supercritical fluid chromatography, can be used. Supercritical chromatography has barely been applied for pesticide matrices. In this paper, an analytical method using coupled to a photodiode array detection established first time quantification papaya and avocado. The extraction methyl parathion, atrazine, ametryn, carbofuran, carbaryl was performed...
Cutaneous loxoscelism envenomation by Loxosceles spiders is characterized the development of a dermonecrotic lesion, strong inflammatory response, production pro-inflammatory mediators, and leukocyte migration to bite site. The role phospholipase D (PLD) from in recruitment monocytes site has not yet been described. This study reports on expression profiles cytokines chemokines human skin fibroblasts treated with catalytically active inactive recombinant PLDs laeta (rLlPLD) lipid mediators...