A recombinant DNA polymerase derived from the thermophilic eubacterium Thermus thermophilus (Tth pol) was found to possess very efficient reverse transcriptase (RT) activity in presence of MnCl2. Many problems typically associated with high degree secondary structure present RNA are minimized by using a thermostable for transcription, and predominantly full-length products can be obtained. The cDNA also amplified chain reaction (PCR) same enzyme. Tth pol observed greater than 100-fold more...

Amplification of RNA by the polymerase chain reaction (PCR) is normally a two-step process requiring separate enzymes and buffer conditions. We describe combined reverse transcription-PCR (RT-PCR) assay for hepatitis C virus (HCV) amplification in which single enzyme condition are used. In this assay, both RT PCR steps carried out with thermoactive DNA Thermus thermophilus. A transcription vector containing HCV sequences has also been constructed to generate quantifiable templates that can...

Selective conversion of formic acid to H₂ and CO₂ is catalysed by a molecular aluminum complex. Metal–ligand cooperative interactions stabilize transition state for an outer-sphere β-hydride abstraction mechanism catalysis.

The synthesis and characterization of air stable Fe(II) coordination complexes with tetrazine triazolo-tetrazine ligands perchlorate counteranions have been achieved. Time-dependent density functional theory (TD-DFT) was used to model the structural, electrochemical, optical properties these materials. These compounds are secondary explosives that can be initiated Nd:YAG laser light at lower energy thresholds than those PETN. Furthermore, significantly sensitivity PETN toward mechanical...

Activation of N–H bonds by a molecular aluminum complex via metal–ligand cooperation is described. (PhI2P2–)AlH (1b), in which PhI2P2– tridentate bis(imino)pyridine ligand, reacts with anilines to give the N–H-activated products (PhHI2P–)AlH(NHAr) (2). When heated, 2 releases H2 and affords (PhI2P–)Al(NHAr) (3). Complex 1b catalyzes dehydrogenative coupling benzylamine afford H2, NH3, N-(phenylmethylene)benzenemethanamine.

Electrophilic activation and subsequent reduction of substrates is in general not possible because highly Lewis acidic metals lack access to multiple redox states. Herein, we demonstrate that transition metal-like processes electronic structure magnetic properties can be imparted aluminum(III). Bis(iminopyridine) complexes containing neutral, monoanionic, dianionic iminopyridine ligands (IP) have been characterized structurally electronically; yellow (IP)AlCl(3) (1), deep green...

Abstract The synthesis of two four‐coordinate and square planar (SP) complexes aluminum(III) is presented. Reaction a phenyl‐substituted bis(imino)pyridine ligand that reduced by electrons, Na 2 ( Ph I P 2− ), with AlCl 3 afforded five‐coordinate [( )Al(THF)Cl] 1 ). Square‐planar )AlCl] ) was obtained performing the same reaction in diethyl ether followed lyphilization from benzene. geometry index for , τ 4 0.22, where 0 would be perfectly square‐planar molecule. analogous aluminum hydride...

A proton shuttle in the second coordination sphere of [Fe₄N(CO)₁₂]⁻promotes H₂evolution over formate formation from CO₂.

The sensitivity of explosives is controlled by factors that span from intrinsic chemical reactivity to mesoscale structure, and has been a topic extensive study for over 50 years.

The synthesis and characterization of new 1,2,4-triazolyl 4-nitro-pyrazolyl substituted tetrazine ligands has been achieved. strongly electron deficient did not coordinate Fe(II) metal centers, while the mildly centers in a 2:1 ratio ligand to metal. thermal stability mechanical sensitivity characteristics complexes are similar conventional explosive pentaerythritol tetranitrate. had strong absorption visible region spectrum that extended into near-infrared. In spite having improved oxygen...

Spin crossover complexes are known to undergo bond length, volume, and enthalpy changes during spin transition. In an explosive complex, these could affect the mechanical initiation sensitivity of lead development a new class switchable materials. To explore this relationship, well-known compound [Fe(Htrz)3]n[ClO4]2n (1) was re-evaluated for its properties, impact correlated A variable temperature test developed used evaluate 1 in low (LS, S = 0), thermally accessed high (HS, 2), mixed LS HS...

Hydrogen abstraction by aluminum(III)-oxo intermediates via reaction pathways reminiscent of late transition metal chemistry has been observed. Oxidation M(+)[(IP(2-))(2)Al](-) (IP = iminopyridine, M Na, Bu(4)N) yielded [Na(THF)(DME)][(IP(-))(IP(2-))Al(OH)] (3) or [(IP(-))(2)Al(OH)] (4), O-atom transfer and subsequent C-H activation proton abstraction, respectively.

Analysis of fractions containing purified DNA polymerase epsilon from calf thymus has revealed the presence a 5' to 3' exonuclease activity that is specific for single strand duplex DNA. This capable degrading 3'-labeled oligonucleotide hybridized M13mp18 When second primer annealed 3 bases upstream, degradation downstream strictly dependent on synthesis upstream primer. Replacement by an oligoribonucleotide identical sequence results in similar pattern exonucleolytic activity. The been...

Redox active ligands are shown to facilitate a variety of group transfer reactions at redox inert aluminum(III). Disulfides can be used as two-electron reagent, and we show that (IP(-))(2)AlSR formed by reaction [(THF)(6)Na][(IP(2-))(2)Al] (1c) with disulfides RSSR (where X = C(S)NMe(2), 4; SMe, 5). In more general route substitution aluminum bis(iminopyridine) complexes, report zinc(II) salts reagent. Reaction [((R)IP(2-))(2)Al](-) (R H, 1c; Me, 1d) ZnX(2) affords ((R)IP(-))(2)AlX IP...

The combination of an electrophilic metal center with a redox active ligand set has the potential to provide reactivity unique from transition chemistry. In this report, substituted iminopyridine complexes containing monoanionic and dianionic (Me)IP(Mes) ligands have been characterized structurally electronically. Green ((Me)IP(Mes)(-))AlCl(2) (1), ((Me)IP(Mes)(-))AlMe(2) (2), ((Me)IP(Mes)(-))GaCl(2) (5) doublet spin state which results anion radical form (Me)IP(Mes). Purple...

Phenyl-bis(imino)pyridine (PhI2P) complexes, (PhI2P)ZnCl2 (1), (PhI2P−)ZnCl (2) and (PhI2P−)Zn(py)Cl (3) were obtained with the I2P ligand in both neutral one-electron reduced state. In all examples, metal ion is Zn(II). Metrical parameters from solid state structures of 2 3 indicate that PhI2P− exists as a radical which supported at carbon atom imino donor, this electronic also apparent analogous Al(III) complex, (PhI2P−)AlCl2 (4), we prepared for comparison. We unable to obtain PhI2P Mg so...

Abstract The engineering of high-performance enzymes for future sequencing and PCR technologies as well the development many anticancer drugs requires a detailed analysis DNA/RNA synthesis processes. However, due to complex molecular interplay involved, real-time methodologies have not been available obtain comprehensive information on both binding parameters enzymatic activities. Here we introduce chip-based method investigate polymerases their interactions with nucleic acids, which employs...

Addition of the O–H bonds in water across aluminum–nitrogen bond a molecular aluminum–amido complex affords an alumoxane. The reaction (PhI2P2–)AlH (1) with forms dimeric [(PhHI2P–)AlH](μ-O) (2) under mild conditions. Upon 2 excess [(PhHI2P–)Al(OH)](μ-O) (3) is formed liberation H2.

We present laser-driven shock Hugoniot measurements of single-crystal (SC) 1,3,5-triamino-2,4,6-trinitrobenzene (TATB) between 15 and 83 GPa, spanning pressures below well above the Chapman–Jouguet pressure ∼28 GPa for TATB formulations (TATB grains mixed with plastic binders at 5–10 wt. %). The new SC data are generally ∼3% more compressible than previously published on neat formulated measured in gas-gun explosive-driven experiments. An exception is compressions density ∼1.5 (∼30–40 GPa),...

Redox-active Group 13 molecules possess the unusual combination of concomitant redox and acid-base reactivity. These combined properties enable regeneration a metal hydroxide complex in cycle for conversion CO2 into carbonate salts. Reaction (IP(-))2Al(OH) (M = Al, Ga) with 1 atm affords [(IP(-))2Al]2(μ(2)κ(1):κ(2)-OCO2). Subsequent reduction MgCO3 or CaCO3 two equivalents [(IP(2-))2Al](-), which can be reoxidized to close cycle.

Reaction of M+[(IP2-)2Ga]− (IP = iminopyridine, M Bu4N, 1a; (DME)3Na, 1b) with pyridine N-oxide affords two-electron-oxidized (IP–)2Ga(OH) (2) in reactions where the product outcome is independent cation identity, M+. In a second example net two-electron chemistry, outer sphere oxidation using either 1 or 2 equiv one-electron oxidant ferrocenium afforded [(IP–)2Ga]+ (3) 44 87% yield, respectively. TEMPO, oxidant, (IP–)2Ga(TEMPO) (4). Reduction 2IP by 3Na and subsequent reaction GaCl3 yielded...