A5053990482- Toxoplasma gondii Research Studies
- Cytomegalovirus and herpesvirus research
- Mosquito-borne diseases and control
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Signaling Pathways in Disease
- Heme Oxygenase-1 and Carbon Monoxide
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Herpesvirus Infections and Treatments
- Click Chemistry and Applications
- RNA and protein synthesis mechanisms
- Advanced Proteomics Techniques and Applications
- HIV Research and Treatment
- Peptidase Inhibition and Analysis
- Redox biology and oxidative stress
- Chemical Synthesis and Analysis
- Insect symbiosis and bacterial influences
- RNA Interference and Gene Delivery
Imperial College London
2018-2022
Lancaster University
2018
Abstract Nucleophilic amino acids are important in covalent drug development yet underutilized as anti-microbial targets. Chemoproteomic technologies have been developed to mine chemically accessible residues via their intrinsic reactivity towards electrophilic probes but cannot discern which reactive sites contribute protein function and should therefore be prioritized for discovery. To address this, we a CRISPR-based oligo recombineering (CORe) platform support the rapid identification,...
The ability of an organism to sense and respond environmental redox fluctuations relies on a signaling network that is incompletely understood in apicomplexan parasites such as Toxoplasma gondii . impact changes upon the development this intracellular parasite not known. Here, we provide revised collection 58 genes containing domains related canonical antioxidant function, with their encoded proteins widely dispersed throughout different cellular compartments. We demonstrate addition...
Cellular barcoding techniques are powerful tools to understand microbial pathogenesis. However, strategies have not been broadly applied protozoan parasites, which unique genomic structures and virulence compared with viral bacterial pathogens. Here, we present a CRISPR-based method barcode protozoa, successfully apply Toxoplasma gondii Trypanosoma brucei. Using libraries of barcoded T. gondii, evaluate shifts in the population structure from acute chronic infection mice. Contrary...
The bloodstream form of the parasite Trypanosoma brucei obtains iron from its mammalian host by receptor-mediated endocytosis transferrin through own unique receptor (TbTfR). Expression TbTfR rapidly increases upon starvation post-transcriptional regulation a currently undefined mechanism that is distinct response system. We have created reporter cell lines fusing 3’UTR or control Aldolase to genes encoding GFP firefly Luciferase, and inserted fusions into line at tagged ribosomal RNA locus....
Method2 June 2020Open Access Transparent process CRISPR-TAPE: protein-centric CRISPR guide design for targeted proteome engineering Daniel Paolo Anderson orcid.org/0000-0003-4422-9520 Department of Life Sciences, Imperial College London, UK Search more papers by this author Henry James Benns orcid.org/0000-0003-3757-7404 Chemistry, Edward William Tate orcid.org/0000-0003-2213-5814 Matthew Andrew Child Corresponding Author [email protected] orcid.org/0000-0002-1565-5638 Information...
Abstract The bloodstream form of the parasite Trypanosoma brucei obtains iron from its mammalian host by receptor-mediated endocytosis transferrin through own unique receptor ( Tb TfR). Expression TfR rapidly increases upon starvation post-transcriptional regulation a currently undefined mechanism that is distinct response system. We have created reporter cell lines fusing 3′UTR or control Aldolase 3’UTR to genes encoding GFP firefly Luciferase, and inserted fusions into line at tagged...
Abstract Molecular barcoding techniques have emerged as powerful tools to understand microbial pathogenesis. However, strategies not been extended protozoan parasites, which unique genomic structures and virulence compared viral bacterial pathogens. Here, we present a versatile CRISPR-based method barcode protozoa, successfully apply Toxoplasma gondii Trypanosoma brucei . The murine brain is an important transmission niche for T. , persistence clinically untreatable feature of infection....
Abstract The ability of an organism to sense and respond environmental redox fluctuations relies on a signaling network that is incompletely understood in apicomplexan parasites such as Toxoplasma gondii . impact changes upon the development this intracellular parasite not known. Here, we provide revised collection 58 genes containing domains related canonical antioxidant function, with their encoded proteins widely dispersed throughout different cellular compartments. We demonstrate...
Molecular barcoding techniques have emerged as powerful tools to understand microbial pathogenesis. However, strategies not been extended protozoan parasites, which unique genomic structures and virulence compared viral bacterial pathogens. Here, we present a versatile CRISPR-based method barcode protozoa, successfully apply Toxoplasma gondii Trypanosoma brucei. The murine brain is an important transmission niche for T. gondii, persistence clinically untreatable feature of infection....