A5064759478- Aquaculture Nutrition and Growth
- Reproductive biology and impacts on aquatic species
- Crustacean biology and ecology
- Fish Ecology and Management Studies
- Sperm and Testicular Function
- Marine Biology and Ecology Research
- Marine and fisheries research
- Invertebrate Immune Response Mechanisms
- Neurobiology and Insect Physiology Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Aquaculture disease management and microbiota
- Fish Biology and Ecology Studies
- Cephalopods and Marine Biology
- Reproductive Biology and Fertility
- Physiological and biochemical adaptations
- Zebrafish Biomedical Research Applications
- Environmental Toxicology and Ecotoxicology
- Parasite Biology and Host Interactions
- Advanced Proteomics Techniques and Applications
- Heat shock proteins research
- Retinal Development and Disorders
- Botany, Ecology, and Taxonomy Studies
- Nanoparticles: synthesis and applications
- Echinoderm biology and ecology
- Cholesterol and Lipid Metabolism
South Bohemia research center of aquaculture and biodiversity of hydrocenoses
2014-2024
University of South Bohemia in České Budějovice
2011-2024
Sewanee: The University of the South
2012-2015
Shahid Beheshti University
2009-2011
University of Tehran
2006-2007
Abstract The quality of frozen/thawed fish sperm is generally lower than that fresh sperm. Extremely low temperatures are associated with damage to spermatozoon subcellular compartments and molecules such as DNA proteins. Cryodamage can negatively affect integrity, metabolism motility, consequently impairing fertilization embryo development. To preserve efficiently, the addition proteins including seminal plasma proteins, antioxidants, antifreeze glycoproteins, bovine serum albumin before...
Biological invasions are increasingly recognized ecological and economic threats to biodiversity projected increase in the future. Introduced freshwater crayfish particular protruding invaders, exerting tremendous impacts on native ecosystem functioning, as exemplified by North American spiny-cheek, signal red swamp well Australian common yabby. The marbled is among most outstanding invaders due its parthenogenetic reproduction combined with early maturation high fecundity. As their...
Morphology of the crayfish spermatozoon and spermatophore wall during three stages final maturation including freshly ejaculated, post-mating, after spermatozoa release was studied compared. The consists a sperm mass enveloped by layered wall. After mating, thickness outer layer is increased. matrix in middle becomes reticulated, granules inside this their contents. Fibers inner degrade to small particles. capsule swells space between appears. area plasma membrane increased wrinkling surface...
Proteins of the signal crayfish Pacifastacus leniusculus egg and spermatophore were identified using in-gel digestion, mass spectrometry, Mascot search. Forty-one one-hundred-fifty proteins in spermatophore, respectively. The classified into nine categories including cell defence, signaling, cytoskeleton, DNA related activity, metabolism energy production, protease inhibitor, respiration, transportation, others unknown. Twenty-two found both spermatophore. respiration cytoskeleton groups are...
Abstract This study reports about the spermatozoal ultrastructure of three species astacid crayfish, i.e., stone crayfish Austropotamobius torrentium , signal Pacifastacus leniusculus and noble Astacus astacus . The acrosome is a cup shaped electron‐dense structure at anterior spermatozoon comprises layers differing electron densities filled with parallel filaments that extend from base to apical zone. was significantly longer in A than P shortest belongs width narrower widest A. L:W ratio...
Heat shock proteins (HSPs) are highly conserved, cytoprotective proteins, the best know function of which is to facilitate proper folding nascent proteins. Environmental stressors, including abiotic and biotic, induce damage cell's activating an adaptive response in any aquatic organism. In expression specific HSP-encoding genes significantly enhanced across different crustacean species. Functional studies crustaceans have suggested that HSPs not only mediate chaperone-cytoprotective...
Sperm density, mineral and organic composition of the seminal plasma their physiological relationship were investigated in Caspian brown trout (Salmo trutta caspius). To establish a rapid accurate method for assessment sperm three different techniques used: counting, spectrophotometry (at 480 nm) determination spermatocrit. The contained 159.26±8.84 mM sodium (Na), 33.72±2.01 potassium (K), 133.04±5.96 chlorine (Cl), 1.68±0.2 calcium (Ca) 0.988±0.13 magnesium (Mg). following components...
The freshly ejaculated spermatophore of crayfish undergoes a hardening process during post-mating storage on the body surface female. ultrastructural distribution calcium deposits were studied and compared in noble spermatophores, using oxalate-pyroantimonate technique, to determine possible roles spermatozoon maturation. Small particles sparsely distributed visible wall spermatophore. Also, large amount membranes spermatozoon. Five minutes post-ejaculation, granules appeared as porous...
Abstract Calcium plays a variety of vital regulatory functions in many physiological and biochemical events the cell. The aim this study was to describe ultrastructural distribution calcium during different developmental stages spermatogenesis model organism, zebrafish ( Danio rerio ), using combined oxalate–pyroantimonate technique. Samples were treated by potassium oxalate pyroantimonate two fixation examined transmission electron microscopy detect dense intracellular calcium. subcellular...
Abstract The changes in some of the biological traits mature females critically endangered Caspian Brown Trout, Salmo trutta caspius Dorofeeva, 1967, during recent decades were analysed. results demonstrated that mean length has significantly (p<0.05) declined over past 60 years, from 77.6 cm 1947 to 59.8 2007 (22.4%). same trend was observed for total weight which reduced 4880.2 g 2486.8 (49.5%). Also, level absolute fecundity sharply decreased 7041.8 4526.1 1973 and 2941.2 1986. Whereas...
Abstract Calcium regulates many intracellular events such as growth and differentiation during different stages of gamete development. The aim this study was to localize quantify the distribution calcium developmental spermatogenesis in sterlet, Acipenser ruthenus , using a combined oxalate–pyroantimonate technique. described spermatogonium, spermatocyte, spermatid, spermatozoon stages. In spermatogonium deposits were mainly localized nucleus cytoplasm. spermatid had nucleus, developing...
Female crayfish stores male gametes after mating until the beginning of egg laying and fertilization. The aim present study was to investigate duration post-mating spermatophore storage as well timing temperature spawning in two species economic importance, namely signal Pacifastacus leniusculus noble Astacus astacus. Results showed that average is significantly (P<0.05) longer (34.6±1.7 days, range: 19 60 days) than (3.9±0.5 1 18 days). highest percentages (46.5%) (44.5%) were 31 40...