To investigate the coupling selectivity of G proteins and protein-coupled receptors (GPCRs), we developed a reconstitution system made up GPCR heterotrimeric on extracellular baculovirus particles (budded virus (BV)). BV released from Sf9 cells infected with recombinant coding for human leukotriene B4 receptor (BLT1) cDNA exhibited high level BLT1 expression (27.3 pmol/mg protein) specific [3H]leukotriene binding activity (Kd = 3.67 nm). The apparent low affinity expressed is thought to be...

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication the EditorNEXTSide-Chain Polypseudorotaxanes with Heteromacrocyclic Receptors of Cyclodextrins (CDs) and Cucurbit[7]uril (CB7): Their Contrast Lower Critical Solution Temperature Behavior α-CD, γ-CD, CB7Tomoki Ogoshi*, Kazuyuki Masuda, Tada-aki Yamagishi, Yoshiaki NakamotoView Author Information Graduate School Natural Science Technology, Kanazawa University, Kakuma-machi, 920-1192, Japan*Corresponding author: Tel...

The cDNA for leukotriene B4 (LTB4) receptor (BLT) was cloned from a guinea-pig leucocyte library. encodes 348 amino acid residues and shares 73% identity with the sequence of human BLT. Northern blot analysis showed highest expression mRNA in leucocytes, followed by lung spleen. membrane fractions HEK-293 Cos-7 cells transfected specific LTB4-binding activities, Kd values 0.27 0.17 nM respectively. Xenopus laevis oocytes injected cRNA BLT LTB4-induced Cl- currents, indicating that is...

We have successfully printed wires of 5 µm line widths and spaces in copper nanoparticle ink with a super-inkjet printer. The show resistivity as low 8.1 µΩ·cm after sintering at 250 °C an extremely oxygen atmosphere generated by pump. To our knowledge, this is the first report micron-scale wiring formed direct printing method without any masks or templates.

Despite that recent progress in genomics has elucidated the genomic structure of olfactory receptors (ORs), most them are still orphan receptors. The low expression level ORs heterologous cells hampered many attempts to establish cell biological OR assay systems. Recently, we demonstrated certain G protein-coupled receptors, such as leukotriene B4 receptor or dopamine D1 receptor, were efficiently reconstituted on baculovirus budding from infected Sf9 cells. budded virus (BV) was shown be...

We prepared MgB2/Ni and MgB2/B alternately-layered films using electron beam evaporation coaxial vacuum arc techniques without any post-annealing. The thickness of each MgB2 layer was designed to be 42, 24 or 15 nm for both films. confirmed that the layered structure successfully obtained from scanning transmission microscopic cross-sectional image film. critical temperature, Tc, film decreased as became thinner, Moreover, Tc affected by only thickness, independent inserted materials.

With special reference to skeletal growth, a 17-year follow-up study of lower-leg replantation in four-year-old boy is reported. The patient maintained good cosmesis and function; however, foot size on the affected side was 1.5 cm smaller than contralateral side, leg length 1.2 shorter normal side. A Cybex II disclosed that had almost half-standard strength evertors at 30 deg/sec plantar flexors 20 120 involved According these findings late replanted child, growing child apparently has...

In this paper, we will show the superconducting properties of alternately-layered MgB <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> /Ni thin films. multilayered films were prepared on silicon substrates (100) by sequentially switching an electron beam evaporation technique and a coaxial vacuum arc source. The lattice spacing artificial pinning layers was designed to correspond magnetic field 8 T (flux line 15 nm). As result, clear...

We prepared MgB <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> thin films by an electron beam evaporation technique and found that either vacancies and/or B substitutions on Mg sites worked as effective pinning centers in . also the grain boundaries formed columnar grains work centers, measuring transport <i xmlns:xlink="http://www.w3.org/1999/xlink">J</i> xmlns:xlink="http://www.w3.org/1999/xlink">c</sub> 's changing relative angle...

MgB <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> thin films were prepared on polycrystalline Cu substrates by using an electron beam evaporation technique. The J xmlns:xlink="http://www.w3.org/1999/xlink">c</sub> of film plate reached very high about 1 times 10 <sup xmlns:xlink="http://www.w3.org/1999/xlink">7</sup> A/cm xmlns:xlink="http://www.w3.org/1999/xlink">2</sup> at 4.2 K and T. We think that this result may show the...

Cell migration to chemoattractants is critically important in both normal physiology and the pathogenesis of many diseases. In GPCR-mediated chemotaxis, GPCRs transduce gradient an extracellular chemotactic ligand into intracellular responses via activation heterotrimeric G proteins. However, ligand-induced G-protein has not been directly imaged as yet mammalian chemotaxing cells. We developed a Förster resonance energy transfer (FRET) probe, R10-Gi, by linking Gi-protein α subunit regulator...

In this paper, we prepared alternately layered MgB <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> /B examine the superconducting properties of multilayered thin films inserting a very layer boron between . The xmlns:xlink="http://www.w3.org/1999/xlink">2/</sub> B were successfully using electron beam evaporation technique without any post-annealing. No inter-diffusion magnesium and can be detected in consist individual layers.

We have analyzed the core promoter element of Na + /K ‐ATPase α1 subunit gene by means an in vitro transcription system composed a HeLa nuclear extract. 5′‐deletion and 3′‐deletion analyses revealed that this is specifically transcribed RNA polymerase II manner dependent on upstream regulatory region (−102 to −61), 3′ boundary minimal does not extend beyond +5. Analysis linker‐substitution mutations point TATA‐like sequence (−33 −26) required for upstream‐sequence‐dependent whereas near +1...

The cDNA for leukotriene B4 (LTB4) receptor (BLT) was cloned from a guinea-pig leucocyte library. encodes 348 amino acid residues and shares 73% identity with the sequence of human BLT. Northern blot analysis showed highest expression mRNA in leucocytes, followed by lung spleen. membrane fractions HEK-293 Cos-7 cells transfected specific LTB4-binding activities, Kd values 0.27 0.17 nM respectively. Xenopus laevis oocytes injected cRNA BLT LTB4-induced Cl- currents, indicating that is...