A5015255932- Advanced machining processes and optimization
- Manufacturing Process and Optimization
- Reproductive System and Pregnancy
- Advanced Machining and Optimization Techniques
- Injection Molding Process and Properties
- Bacterial Genetics and Biotechnology
- Endometriosis Research and Treatment
- Microfluidic and Bio-sensing Technologies
- Additive Manufacturing and 3D Printing Technologies
- Orbital Angular Momentum in Optics
- Bacteriophages and microbial interactions
- Surface Roughness and Optical Measurements
- Pregnancy-related medical research
- Legume Nitrogen Fixing Symbiosis
- Quality and Supply Management
- Advanced Measurement and Metrology Techniques
- RNA and protein synthesis mechanisms
- Advanced Surface Polishing Techniques
- Meningioma and schwannoma management
- Fuzzy Logic and Control Systems
- Plant nutrient uptake and metabolism
- Industrial Vision Systems and Defect Detection
- Glioma Diagnosis and Treatment
- Engineering Technology and Methodologies
- Advanced Manufacturing and Logistics Optimization
BioMarin (United States)
2019-2025
Rapt Therapeutics (United States)
2025
San Francisco State University
2013-2024
Bradley University
2012-2024
Courant Institute of Mathematical Sciences
2024
New York University
2024
University of California, San Francisco
2011-2022
Renfe Operadora (Spain)
2021
DePaul University
2013-2020
Joint BioEnergy Institute
2018
Assembly of the division septum in bacteria is mediated by several proteins that localize to site. One these, FtsI (also called penicillin-binding protein 3) Escherichia coli, consists a short cytoplasmic domain, single membrane-spanning segment, and large periplasmic domain encodes transpeptidase activity involved synthesis septal peptidoglycan. We have constructed merodiploid strain with wild-type copy ftsI at normal chromosomal locus genetic fusion green fluorescent (gfp) lambda...
Though lean manufacturing has been widely recognised for its effectiveness in continuously improving productivity, product quality, and on-time delivery to customers, the cost hiring a full-time engineer kept many small businesses from implementing their facilities. This paper presents case study of implementation at manufacturer United States. Starting with collecting process information, current value-stream map was created that reflected operation status. A future value stream then...
Human endometrium undergoes cyclic regeneration involving stem/progenitor cells, but the role of resident endometrial mesenchymal stem cells (eMSC) as progenitors stromal fibroblasts (eSF) has not been definitively demonstrated. In endometriosis, eSF display progesterone (P4) resistance with impaired decidualization in vivo and vitro. To investigate eMSC precursors whether endometriosis P4 is inherited from eMSC, we analyzed transcriptomes eutopic isolated by fluorescence-activated cell...
In the discourse on diversity in colleges and universities United States, an often-neglected population is nontraditional adult learners. This article explores this invisible aspect of undergraduate diversity, addresses how competence-based education, which focuses demonstrating actual ability to do, innovative approach that caters learners’ life phase learning needs. College arguably a youth-centric generally designed for younger student. However, stereotypical full-time student who lives...
During cell division in Gram-negative bacteria, the envelope invaginates and constricts at septum, eventually severing into two compartments, separating replicated genetic materials. In Escherichia coli, least nine essential gene products participate directly septum formation: FtsA, FtsI, FtsL, FtsK, FtsN, FtsQ, FtsW, FtsZ ZipA. All proteins have been localized to septal ring, an equatorial ring structure site. We used translational fusions green fluorescent protein (GFP) demonstrate that...
ABSTRACT We describe a simple system for reversible, stable integration of plasmid-borne genes into the Escherichia coli chromosome. Most ordinary E. strains and variety pBR322-derived ampicillin-resistant plasmids can be used. A single genetic element, lambda phage, is only specialized vector required. The resultant have copy plasmid fragment inserted stably at attachment site on chromosome, with nearly entire genome deleted.
Summary Caulobacter crescentus assembles many of its cellular machines at distinct times and locations during the cell cycle. PodJ provides spatial cues for biogenesis several polar organelles, including pili, adhesive holdfast chemotactic apparatus, by recruiting structural regulatory proteins, such as CpaE PleC, to a specific pole. is protein with single transmembrane domain that exists in two forms, full‐length (PodJ L ) truncated S ), each appearing time period cycle control different...
As the USA experiences rapid growth of nontraditional adult students in higher education, educators and institutions will increasingly need to look beyond traditional youth-centric educational models better address learning needs. To date, no research has been conducted examining enrolled a disciplinary course that was built upon core principles learning. Ten (mean age = 45.4 years), an American university with college dedicated students, were interviewed assess their felt impact after...
This paper presents a look at the concept of Lean Manufacturing as applied to office environment. Manufac- turing, which is often referred Lean, philosophy manufacturing process improvement through elimina- tion waste. As developed since its origins in automobile industry, it has been extended apply various enterprises and organizations, achieved renowned reputation for reducing waste while adding value prod- ucts services. also environment; however, participants implementation Office face...
Septation in Escherichia coli requires several gene products. One of these, FtsQ, is a simple bitopic membrane protein with short cytoplasmic N terminus, membrane-spanning segment, and periplasmic domain. We have constructed merodiploid strain that expresses both FtsQ the fusion green fluorescent (GFP)-FtsQ from single-copy chromosomal genes. The gfp-ftsQ complements null mutation ftsQ. Fluorescence microscopy revealed GFP-FtsQ localizes to division site. Replacing transmembrane domains...
We report a method to estimate the restoring force of constrained small molecule and relate changes in its reactivity this force. Restoring is size-invariant measure molecular strain offers conceptual framework bridge studies macromolecular reactions by spectroscopies with those strained molecules. illustrate our showing that E stiff stilbene (1,1'-Delta-biindan) up 700 pN along C6,C6' axis accessible photoisomerization. It reverts thermally Z isomer tau(1/2) approximately 100 ms, probably...
This article presents a study where the Taguchi Design was applied to optimize surface quality in computer numerical control (CNC) drilling operation. The factors this included feed rate, spindle speed, peck and tool type while noise simulated were shop vibration presence or absence of magnetism workpiece material. Through statistical analysis response variables signal-to-noise ratios, determined significant type, optimal combination cutting parameters selected. Confirmation tests verified...
STUDY QUESTIONHow does seminal plasma (SP) affect the transcriptome of human primary endometrial epithelial cells (eEC) and stromal fibroblasts (eSF)?
Knowledge-based environmental education has been consistently found to be limited in its effectiveness due small and indirect role promoting sustainable behavior, while focusing on the impact of social context more conceptually relevant. Transformative learning theory is positioned foundational based increasing focus activism through personal transformations. We suggest four criteria development an effective transformative methodology. Methodologies should (1) change not just knowledge...
Understanding early events of HIV transmission within mucosal tissues is vital for developing effective prevention strategies. Here, we report that primary stromal fibroblasts isolated from endometrium, cervix, foreskin, male urethra, and intestines significantly increase infection CD4+ T cells–by up to 37-fold R5-tropic 100-fold X4-tropic HIV–without themselves becoming infected. Fibroblasts were more efficient than dendritic cells at trans-infection mediate this response in the absence...
Many efforts to improve science teaching in higher education focus on a few faculty members at an institution time, with limited published evidence attempts engage across entire departments. We created long-term, department-wide collaborative professional development program, Biology Faculty Explorations Scientific Teaching (Biology FEST). Across 3 years of FEST, 89% the department's completed weeklong scientific institute, and 83% eligible instructors participated additional semester-long...
Abstract Tightly regulated promoters are essential for numerous biological applications, where strong inducibility, portability, and scalability desirable. Current systems often incompatible with large-scale fermentations due to high inducer costs strict media requirements. Here, we describe the bottom-up engineering of ‘Jungle Express’, an expression system that enables efficient gene regulation in diverse proteobacteria. This is guided by EilR, a multidrug-binding repressor affinity its...
In Escherichia coli , nine gene products are known to be essential for assembly of the division septum. One these, FtsL, is a bitopic membrane protein whose precise function not understood. Here we use fluorescence microscopy study subcellular localization both in wild‐type strain and merodiploid that expresses GFP–FtsL fusion protein. We show FtsL localizes cell septum where it forms ring analogous cytoplasmic FtsZ ring. dependent upon FtsZ, FtsA FtsQ, but FtsI. reverse approach, fusions...