A5002150589- Amino Acid Enzymes and Metabolism
- Metabolism and Genetic Disorders
- Drug Transport and Resistance Mechanisms
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Trace Elements in Health
- Folate and B Vitamins Research
- Neuroscience and Neuropharmacology Research
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Endoplasmic Reticulum Stress and Disease
- Biomedical Research and Pathophysiology
- Enzyme Structure and Function
- Cancer, Hypoxia, and Metabolism
- Antibiotic Resistance in Bacteria
- Chemical Synthesis and Analysis
- Peroxisome Proliferator-Activated Receptors
- Pharmacological Effects and Toxicity Studies
- Diet and metabolism studies
- Polyamine Metabolism and Applications
- Bacteriophages and microbial interactions
- ATP Synthase and ATPases Research
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Ion channel regulation and function
University of Calabria
2016-2025
Centre International de Recherche sur le Cancer
2011
Article2 November 2022Open Access Source DataTransparent process TMBIM5 is the Ca2+/H+ antiporter of mammalian mitochondria Shane Austin orcid.org/0000-0002-8698-6055 Department Internal Medicine I and Comprehensive Cancer Center, Medical University Vienna, Austria Contribution: Conceptualization, Data curation, Formal analysis, Validation, Investigation, Visualization, Methodology, Writing - original draft, review & editing Search for more papers by this author Ronald Mekis Biomedical...
Abstract In bacteria and archaea, tripartite ATP-independent periplasmic (TRAP) transporters uptake essential nutrients. TRAP receive their substrates via a secreted soluble substrate-binding protein. How sodium ion-driven secondary active transporter is strictly coupled to protein poorly understood. Here we report the cryo-EM structure of sialic acid SiaQM from Photobacterium profundum at 2.97 Å resolution. SiaM comprises “transport” domain “scaffold” domain, with transport consisting...
N -Acetylneuraminic acid (Neu5Ac) is a negatively charged nine-carbon amino sugar that often the peripheral in human cell-surface glycoconjugates. Some bacteria scavenge, import, and metabolize Neu5Ac or redeploy it on their cell surfaces for immune evasion. The import of by many mediated tripartite ATP-independent periplasmic (TRAP) transporters. We have previously reported structures SiaQM, membrane-embedded component Haemophilus influenzae TRAP transport system, (Currie et al., 2024)....
Many pathogenic bacteria utilise sialic acids as an energy source or use them external coating to evade immune detection. As such, that colonise sialylated environments deploy specific transporters mediate import of scavenged acids. Here, we report a substrate-bound 1.95 Å resolution structure and subsequent characterisation SiaT, acid transporter from Proteus mirabilis. SiaT is secondary active the sodium solute symporter (SSS) family, which Na
Proteoliposomes represent a suitable and up to date tool for studying membrane transporters which physiologically mediate absorption, excretion, trafficking reabsorption of nutrients metabolites. Using recently developed reconstitution strategies, can be inserted in artificial bilayers with the same orientation as cell membranes absence other interfering molecular systems. These methodologies are very kinetic parameters mechanisms. After first applications on mitochondrial transporters, last...
ABSTRACT Previous studies have shown that the PDZ-binding motif of E6 oncoprotein from mucosal high-risk (HR) human papillomavirus (HPV) types plays a key role in HPV-mediated cellular transformation vitro and vivo experimental models. HR HPV oncoproteins ability to efficiently degrade members PDZ motif-containing membrane-associated guanylate kinase (MAGUK) family; however, it is possible other proteins are also targeted by E6. Here, we describe novel interaction type 16 (HPV16) with...
The human glutamine/neutral amino acid transporter ASCT2 (hASCT2) was over-expressed in Pichia pastoris and purified by Ni(2+)-chelating gel filtration chromatography. protein reconstituted liposomes detergent removal with a batch-wise procedure. Time dependent [(3)H]glutamine/glutamine antiport measured proteoliposomes which active only the presence of external Na(+). Internal Na(+) slightly stimulated antiport. Optimal activity found at pH7.0. A substantial inhibition transport observed...
The large neutral amino acid transporter 1 (LAT1) is a promising anticancer target that required for the cellular uptake of essential acids serve as building blocks cancer growth and proliferation. Here, we report structure-based approach to identify chemically diverse potent inhibitors LAT1. First, homology model LAT1 based on atomic structures prokaryotic homologs was constructed. Molecular docking nitrogen mustards (NMs) with wide range affinity allowed deriving common binding mode could...