A5088138642- Immunotherapy and Immune Responses
- Ocular Oncology and Treatments
- Advanced biosensing and bioanalysis techniques
- Nanoplatforms for cancer theranostics
- Microtubule and mitosis dynamics
- DNA Repair Mechanisms
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Cellular Mechanics and Interactions
- Ubiquitin and proteasome pathways
- Renal and related cancers
- Cancer Genomics and Diagnostics
- Cell Adhesion Molecules Research
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- Cancer-related Molecular Pathways
- Reproductive Biology and Fertility
- Caveolin-1 and cellular processes
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Telomeres, Telomerase, and Senescence
- Plant Genetic and Mutation Studies
- interferon and immune responses
- Retinal Development and Disorders
- Hippo pathway signaling and YAP/TAZ
Université Paris Sciences et Lettres
2021-2024
Inserm
2021-2024
Institut Curie
2021-2024
La Ligue Contre le Cancer
2021-2022
Centre National de la Recherche Scientifique
2007-2020
University of Basel
2017-2020
Institut de Génétique Humaine
2008-2020
Université de Montpellier
2019-2020
Google (United States)
2018
Sorbonne Université
2007
In metazoans, thousands of DNA replication origins (Oris) are activated at each cell cycle. Their genomic organization and their genetic nature remain elusive. Here, we characterized Oris by nascent strand (NS) purification a genome-wide analysis in Drosophila mouse cells. We show that both species most CpG islands (CGI) contain Oris, although methylation is nearly absent , indicating this epigenetic mark not crucial for defining the origin. Initiation synthesis starts borders CGI, resulting...
Disruption of splicing patterns due to mutations genes coding factors in tumors represents a potential source tumor neoantigens, which would be both public (shared between patients) and tumor-specific (not expressed normal tissues). In this study, we show that the factor SF3B1 uveal melanoma generate such immunogenic neoantigens. Memory CD8+ T cells specific for these neoantigens are preferentially found 20% patients with bearing SF3B1-mutated tumors. Single-cell analyses neoepitope-specific...
Article22 March 2018Open Access Transparent process Structural centrosome aberrations promote non-cell-autonomous invasiveness Olivier Ganier Biozentrum, University of Basel, Switzerland Search for more papers by this author Dominik Schnerch Philipp Oertle Swiss Nanoscience Institute, Roderick YH Lim Marija Plodinec Erich A Nigg Corresponding Author [email protected] orcid.org/0000-0003-4835-5719 Information Ganier1,‡, Schnerch1,3,‡, Oertle1,2, Lim1,2, Plodinec1,2 and *,1 1Biozentrum, 2Swiss...
Transfer of somatic cell nuclei to enucleated eggs and ectopic expression specific transcription factors are two different reprogramming strategies used generate pluripotent cells from differentiated cells. However, these methods poorly efficient, other unknown might be required increase their success rate. Here we show that Xenopus egg extracts at the metaphase stage (M phase) have a strong activity on mouse embryonic fibroblasts (MEFs). First, they reset replication properties MEF toward...
Terminal differentiation is the process by which cycling cells stop proliferating to start new specific functions. It involves dramatic changes in chromatin organization as well gene expression. In present report we used cell flow cytometry and genome wide DNA combing investigate replication during murine erythroleukemia-induced terminal differentiation. The results obtained indicated that rate of fork movement slows down inter-origin distance becomes shorter precommitment commitment periods...
Centrosomes determine the disposition of microtubule networks and thereby contribute to regulate cell shape, polarity, motility, as well chromosome segregation during division. Additionally, centrioles, core components centrosomes, are required for formation cilia flagella. Mutations in genes coding centrosomal centriolar proteins responsible several human diseases, foremost ciliopathies developmental disorders resulting small brains (primary microcephaly) or body size (dwarfism). Moreover,...
Centrosome aberrations disrupt tissue architecture and may confer invasive properties to cancer cells. Here we show that structural centrosome aberrations, induced by overexpression of either Ninein-like protein (NLP) or CEP131/AZI1, sensitize polarized mammalian epithelia basal cell extrusion. While unperturbed typically dispose damaged cells through apical dissemination into luminal cavities, certain oncogenic mutations cause a switch in directionality towards extrusion, raising the...
Abstract Background: Cell division or cytokinesis, which results from a series of events starting in metaphase, is the mechanism by mother cell cytoplasm divided between two daughter cells. Hence it final step cycle. The aim present study was to demonstrate that mammalian cells undergoing cytokinesis can be sorted selectively flow cytometry. Materials and Methods: Cultures HeLa were arrested prometaphase nocodazole, collected mitotic shake‐off released for 90 min into fresh medium enrich...
2006 Cold Spring Harbor Cell Cycle Meeting Report
Transition of cytosine to thymine in CpG dinucleotides is the most frequent type mutation cancer. This increased mutability commonly attributed spontaneous deamination 5-methylcytosine (5mC), which normally repaired by base-excision repair (BER) pathway. However, contribution 5mC increasing diversity cancer mutational signatures remains poorly explored. We integrate analysis a large series tumor whole genomes with lineage-specific epigenomic data draw detailed view uncover type-specific...
Meiosis involves two successive rounds of chromosome segregation without an intervening S phase. Exit from meiosis I is distinct mitotic exit, in that replication origins are not licensed by Mcm2-7 chromatin binding, but spindle disassembly occurs during a transient interphase-like state before II. The absence licensing assumed to explain the block DNA replication, this has been formally tested. Here we attempt subvert expressing control factors Cdc18 and Cdt1 interval between meiotic...
SUMMARY DNA replication initiates with pre-replication complex (pre-RC) formation at origins in G1 (replication origin licensing), followed by activation of a pre-RC subset the S phase. It has been suggested that checkpoint prevents phase entry when too few are licensed. Yet, we found normal cells, complete synthesis inhibition overexpression non-degradable geminin variant, or CDT1 silencing without inducing any checkpoint. Cells continue cycling and enter mitosis, despite absence replicated...
Abstract Centrosomes are the main microtubules organizing centers of animal cells. Although centrosome aberrations common in tumors, their consequences remain subject to debate. Here, we studied impact structural aberrations, induced by deregulated expression Ninein-like protein (NLP), on epithelial spheres grown Matrigel matrices. We demonstrate that NLP-induced trigger escape (’budding’) living cells from epithelia. Remarkably, all disseminating into matrix were undergoing mitosis. This...
The TERT/CLPTM1L risk locus on chromosome 5p15.33 is a pleiotropic cancer in which multiple independent alleles have been identified, across well over ten types. We previously conducted genome-wide association study uveal melanoma (UM), uncovered role for the this intraocular tumor and identified highly correlated alleles. Aiming to unravel biological mechanisms UM of locus, contains domain enriched active chromatin marks enhancer elements, we demonstrated allele-specific activity region...
Abstract Inactivating mutations of MBD4 have been reported in subsets various tumors. A deficiency this DNA glycosylase, recognizing specifically T:G mismatch resulting from the deamination methyl-cytosine, results a hypermutated phenotype due to accumulation CpG>TpG transitions. Here, we hypothesize that difference metabolism consecutive may result specific cytotoxicities MBD4-deficient tumor cells synthetic lethality fashion. After large-scale drug repurposing screen, show two isogenic...
<div>Abstract<p>Disruption of splicing patterns due to mutations genes coding factors in tumors represents a potential source tumor neoantigens, which would be both public (shared between patients) and tumor-specific (not expressed normal tissues). In this study, we show that the factor <i>SF3B1</i> uveal melanoma generate such immunogenic neoantigens. Memory CD8<sup>+</sup> T cells specific for these neoantigens are preferentially found 20% patients with...
Supplementary Figure from Splicing Patterns in <i>SF3B1</i>-Mutated Uveal Melanoma Generate Shared Immunogenic Tumor-Specific Neoepitopes