A5063493617- Advanced NMR Techniques and Applications
- Solid-state spectroscopy and crystallography
- Atomic and Subatomic Physics Research
- NMR spectroscopy and applications
- Inorganic Fluorides and Related Compounds
- Crystallography and molecular interactions
- X-ray Diffraction in Crystallography
- Electron Spin Resonance Studies
- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Nuclear Physics and Applications
- Protein Structure and Dynamics
- Alzheimer's disease research and treatments
- Bacteriophages and microbial interactions
- Legume Nitrogen Fixing Symbiosis
- Spacecraft and Cryogenic Technologies
- Gyrotron and Vacuum Electronics Research
- Crystallography and Radiation Phenomena
- Radiation Detection and Scintillator Technologies
- Radioactive element chemistry and processing
- Advanced Battery Materials and Technologies
- Microwave Engineering and Waveguides
- Advanced MRI Techniques and Applications
- Superconducting Materials and Applications
- Mesoporous Materials and Catalysis
Florida State University
2023-2025
National High Magnetic Field Laboratory
2023-2025
Washington University in St. Louis
2015-2019
Solid state nuclear magnetic resonance (NMR) enables atomic-resolution characterization of the molecular structure and dynamics within complex heterogeneous samples, but it is typically insensitive. Dynamic polarization (DNP) increases NMR signal intensity by orders magnitude can be performed in combination with magic angle spinning (MAS) for sensitive, high-resolution spectroscopy. Here we report MAS DNP experiments, first time, intact human cells >40-fold enhancement a sample temperature...
Bacterial biofilms are major contributors to persistent infections and antimicrobial resistance, posing significant challenges treatment. However, obtaining high‐resolution structural information on native bacterial has remained elusive due the methodological limitations associated with analyzing complex biological samples. Solid‐state NMR (ssNMR) shown promise in this regard, but its conventional application is hindered by sensitivity constraints for unlabeled samples . In study, we...
Dynamic nuclear polarization (DNP) can enhance NMR sensitivity by orders of magnitude transferring spin from electron paramagnetic resonance (EPR) to NMR. However, DNP polarizing agents have deleterious effects on signals. Electron decoupling mitigate these relaxation effects. We demonstrate experiments in conjunction with and magic-angle-spinning spectroscopy. Following a diffusion period, the microwave irradiation frequency is quickly tuned on-resonance electrons agent. The performance...
Spherical rotors can be used for magic angle spinning nuclear magnetic resonance.
Bacterial biofilms are major contributors to persistent infections and antimicrobial resistance, posing significant challenges treatment. However, obtaining high‐resolution structural information on native bacterial has remained elusive due the methodological limitations associated with analyzing complex biological samples. Solid‐state NMR (ssNMR) shown promise in this regard, but its conventional application is hindered by sensitivity constraints for unlabeled samples . In study, we...
The contribution of protons in or near biradical polarizing agents Dynamic Nuclear Polarization (DNP) has recently been under scrutiny. Results from selective deuteration and simulations have previously suggested that the role molecule depends on strength electron–electron coupling. Here we use cross effect DNP mechanism to identify acquire 1H solid-state NMR spectra contribute propagation hyperpolarization, via an experimental approach dubbed Nuclear–Nuclear Double Resonance (NUDOR).
With the rapid increase in temperatures around planet, need to develop efficient means reduce CO2 emissions has become one of greatest challenges scientific community. Many different strategies are being studied worldwide, which consists trapping gas into porous materials, either for its short- or long-term capture and storage, re-use production value-added compounds. Yet, further development such systems, there is a real fully understand their structure properties, including at...
With the rapid increase in temperatures around planet, need to develop efficient means reduce CO2 emissions has become one of greatest challenges scientific community. Many different strategies are being studied worldwide, which consists trapping gas porous materials, either for its short- or long-term capture and storage, re-use production value-added compounds. Yet, further development such systems, there is a real fully understand their structure properties, including at molecular-level...
ABSTRACT Cryptococcus species are major fungal pathogens responsible for life-threatening infections in approximately a million individuals globally each year, with alarmingly high mortality rates. These fungi distinguished by distinctive cell wall architecture further reinforced two virulence-associated layers, melanin and capsule, rendering them insensitive to antifungal agents targeting the wall, such as echinocandins. The molecular interplay between these three biomolecular layers...
Non-specific disruption of cellular membranes induced by amyloidogenic aggregation β-amyloid (Aβ) peptides remains a viable cytotoxicity mechanism in Alzheimer's disease (AD). Obtaining structural information about the intermediate states Aβ-membrane systems and their molecular interactions is challenging due to heterogeneity low abundance. Here, we systematically study membrane-associated Aβ1-42 using solid-state nuclear magnetic resonance (ssNMR) spectroscopy, focusing on primary...
The importance of (bi)carbonate salts cannot be understated. They are vital to the Earth’s geology and ecosystems used as precursors by chemists for synthesis functional materials. Naturally, solid-state NMR (ssNMR) appears spectroscopic tool choice probe atomic-level structure dynamics salts. Of possible nuclei available probes in carbonate bicarbonate ions (i.e., ¬1H, 13C, 17O), oxygen-17 is highly attractive. Yet, it seldom employed, largely due its low natural abundance (0.04%) lack...
Dynamic nuclear polarization (DNP) can significantly enhance the sensitivity of NMR spectroscopy, via transfer from unpaired electrons to nuclei. As a result, there is considerable interest in improving DNP efficiency, particularly through development new polarizing agents (PAs). Here, series 32 PAs, including 26 dinitroxide and 6 hetero biradicals, which 11 are introduced for first time, were evaluated experiments at magnetic field strengths 9.4 T, 14.1 21.1 T. The PAs soluble aqueous media...
Dynamic nuclear polarization (DNP) can significantly enhance the sensitivity of NMR spectroscopy, via transfer from unpaired electrons to nuclei. As a result, there is considerable interest in improving DNP efficiency, particularly through development new polarizing agents (PAs). Here, series 32 PAs, including 26 dinitroxide and 6 hetero biradicals, which 11 are introduced for first time, were evaluated experiments at magnetic field strengths 9.4 T, 14.1 21.1 T. The PAs soluble aqueous media...