A5066704814- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Cancer-related Molecular Pathways
- Advanced Proteomics Techniques and Applications
- BRCA gene mutations in cancer
- Molecular Biology Techniques and Applications
- Genomic variations and chromosomal abnormalities
- Gene expression and cancer classification
- Breast Cancer Treatment Studies
- Cervical Cancer and HPV Research
- Pancreatic and Hepatic Oncology Research
- Cell Adhesion Molecules Research
- Thyroid Cancer Diagnosis and Treatment
- Head and Neck Cancer Studies
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- DNA Repair Mechanisms
- Lung Cancer Treatments and Mutations
- Microtubule and mitosis dynamics
- Ovarian cancer diagnosis and treatment
- Cancer Cells and Metastasis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genomics and Chromatin Dynamics
- Breast Lesions and Carcinomas
- Salivary Gland Tumors Diagnosis and Treatment
Karolinska Institutet
2015-2024
Karolinska University Hospital
2014-2023
Helmholtz Zentrum München
2011
Svenska Örtmedicinska Institute
2010
KTH Royal Institute of Technology
2007
Lund University
1986-2007
National Cancer Institute
1978-2004
Center for Cancer Research
2004
Baylor College of Medicine
2004
Institute of Radiobiology of NAS of Belarus
2002
We have chosen tumors of the uterine cervix as a model system to identify chromosomal aberrations that occur during carcinogenesis. A phenotype/genotype correlation was established in defined regions archived, formalin-fixed, and hematoxylin/eosin-stained tissue sections were dissected from normal cervical epithelium (n = 3), mild 4), moderate 6), severe dysplasias/carcinomas situ (CIS) 13), invasive carcinomas 10) investigated by comparative genomic hybridization. The same tissues analyzed...
Comparative genomic hybridization was used to screen the DNA extracted from histologically defined tissue sections consecutive stages of colorectal carcinogenesis for chromosomal aberrations. No aberrations were detected in normal epithelium (n = 14). Gain chromosome 7 occurred as a single event low-grade adenomas In high-grade 12), an overrepresentation chromosomes and 20 present 30% cases analyzed. The transition colon carcinomas 16) characterized by emergence multiple Chromosomes 1, 13,...
Representational Oligonucleotide Microarray Analysis (ROMA) detects genomic amplifications and deletions with boundaries defined at a resolution of approximately 50 kb. We have used this technique to examine 243 breast tumors from two separate studies for which detailed clinical data were available. The very high technology has enabled us identify three characteristic patterns copy number variation in diploid measure correlations patient survival. One these is characterized by multiple...
Microtubule-stabilizing (MTS) agents, such as taxanes, are important chemotherapeutics with a poorly understood mechanism of action. We identified set genes repressed in multiple cell lines response to MTS agents and observed that these overexpressed tumors exhibiting chromosomal instability (CIN). Silencing 22/50 genes, many which involved DNA repair, caused cancer death, suggesting the survival aneuploid cells. Overexpression "CIN-survival" is associated poor outcome estrogen...
Measurement of the nuclear DNA content allows classification human cancers as either diploid or aneuploid. To gain further insight into mechanisms aneuploidy, we compared cytogenetic profile mismatch-repair-deficient versus mismatch-repair-proficient aneuploid colorectal carcinoma cell lines using comparative genomic hybridization and spectral karyotyping. Aneuploid carcinomas revealed an average 19 chromosomal imbalances per line. Such numerical aberrations were exceedingly scarce in...
We have analyzed 30 cases of advanced-stage cervical squamous cell carcinoma (stages IIb-IV) by comparative genomic hybridization (CGH). The most consistent chromosomal gain in the aneuploid tumors was mapped to chromosome arm 3q 77% cases. Acquisition genetic material also occurred frequently on Iq (47%), 5p (30%), 6p (27%), and 20 (23%). Recurrent losses were 2q (33%), 3p (50%), 4 8p (23%), 13q (27%). High-level copy number increases 8, arms 3q, 5p, 8q, 12p, 14q, 17q, 19q, 20p, 20q, bands...
Molecular markers and the rich biological information they contain have great potential for cancer diagnosis, prognostication therapy prediction. So far, however, not superseded routine histopathology staging criteria, partly because few studies performed on molecular subtyping had little validation limited clinical characterization.We obtained gene expression data 412 breast cancers from population-based cohorts of patients Stockholm Uppsala, Sweden. Using intrinsic set approximately 500...
The role of viral oncoprotein expression in the maintenance cellular transformation was examined as a function time through controlled simian virus 40 T antigen (TAg). Expression TAg submandibular gland transgenic mice from birth induced and extensive ductal hyperplasia by 4 months age. reversed when silenced for 3 weeks. When after 7 months, however, persisted even though absent. Although polyploidy cells could be at age, age remained polyploid absence TAg. These results support model...
There is evidence that the extent of G2/M arrest following irradiation correlated with tumour cell survival and hence therapeutic success. We studied regulation cellular response to radiation treatment by miR-21-mediated modulation cycle progression in breast cancer cells analysed miR-21 expression tissue samples long-term follow up. The levels were quantified (qRT-PCR) a panel 86 cases invasive carcinomas relation metastasis free survival. radiosensitivity human after was determined...
Treatment decisions for cervical cancer, a common disease worldwide, depend on demonstrating whether or not tumor invasion of the surrounding tissue has occurred. Invasion can be difficult to assess by standard histopathologic methods, especially when limited amounts are available. Several studies variety cancers have reported increased expression laminin-5-an important attachment protein epithelial cells-in invasive carcinomas. This study was designed investigate presence laminin-5 is...
Four hundred nine consecutive breast cancer patients were studied retrospectively. Microspectrophotometric DNA measurements performed using archival, fine-needle slide preparations upon which the primary diagnoses had been based 8 to 13 years earlier. The distribution patterns of tumor cell populations analyzed according various criteria and cytochemical data correlated clinical course, defined as distant recurrence-free survival. results demonstrated a strong relationship between nuclear...
Recently, expression profiling of breast carcinomas has revealed gene signatures that predict clinical outcome, and discerned prognostically relevant cancer subtypes. Measurement the degree genomic instability provides a very similar stratification prognostic groups. We therefore hypothesized these features are linked. used 48 specimens profoundly differed in their identified set 12 genes defines 2 The biological significance this was established through survival prediction published...
The predictive value of nuclear DNA content in breast cancer relation to clinical and morphologic factors was studied 227 consecutive cases invasive adenocarcinomas with follow-up periods 8 13 years. results show that, the use Cox multivariate analysis, provided significant prognostic information additional that given by all other histomorphologic variables taken together. This fact indicates cells reflects biological properties, associated malignant behavior tumor, than those determining...
Cases of adenoid cystic carcinomas the salivary (n = 90) and lacrimal glands 6) from years 1965–1980 were evaluated retrospectively with regard to clinical, epidemiologic histomorphologic parameters, in 52 cases, nuclear DNA content was assessed using a single cell scanning cytophotometry procedure order determine prognostic factors. Clinical courses poor high incidence recurrences, hematogenous metastases deaths tumor. Histology related prognosis, glandular tumors showing better prognosis...
p53 triggers cell cycle arrest and apoptosis through transcriptional regulation of specific target genes. We have investigated the effect activation on proteome using 2D gel electrophoresis analysis mitomycin C-treated HCT116 colon carcinoma cells carrying wild-type p53. Approximately 5,800 protein spots were separated in overlapping narrow-pH-range strips, 115 showed significant expression changes upon activation. The identity 55 was obtained by mass spectrometry. majority identified...