A5003866771- Genetic factors in colorectal cancer
- Cancer Genomics and Diagnostics
- Colorectal Cancer Treatments and Studies
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Cancer Immunotherapy and Biomarkers
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Colorectal Cancer Surgical Treatments
- Seed Germination and Physiology
- Molecular Biology Techniques and Applications
- PI3K/AKT/mTOR signaling in cancer
- Melanoma and MAPK Pathways
- Cancer-related Molecular Pathways
- Enzyme Catalysis and Immobilization
- Potato Plant Research
- biodegradable polymer synthesis and properties
- Head and Neck Cancer Studies
- RNA and protein synthesis mechanisms
- Advanced Biosensing Techniques and Applications
- Colorectal and Anal Carcinomas
- Bladder and Urothelial Cancer Treatments
- Silk-based biomaterials and applications
- Ear and Head Tumors
- Tumors and Oncological Cases
- Light effects on plants
Universitätsmedizin Rostock
2024-2025
University of Rostock
2009-2024
Hertfordshire Community NHS Trust
2008
Expression of the human endogenous retrovirus (HERV)-H family has been associated with colorectal carcinomas (CRC), yet no individual HERV-H locus expression thoroughly correlated clinical data.Here, we characterized reactivations in CRC samples by integrating profiles, molecular patterns and data. relevant sequences was analyzed qRT-PCR on two well-defined cohorts (n = 139 pairs tumor adjacent normal colon tissue) including from adenomas 21) liver metastases 16). Correlations data were...
The production of biodegradable polymers in transgenic plants order to replace petrochemical compounds is an important challenge for plant biotechnology. Polyaspartate, a substitute polycarboxylates, the backbone cyanobacterial storage material cyanophycin. Cyanophycin, copolymer l-aspartic acid and l-arginine, produced via non-ribosomal polypeptide biosynthesis by enzyme cyanophycin synthetase. A gene from Thermosynechococcus elongatus BP-1 encoding synthetase has been expressed...
Over the time period from 2006 to 2017, consecutive patients operated on at University Medical Center Rostock participated in comprehensive biobanking and tumor-modelling approach known as HROC collection. Samples were collected using strict standard operating procedures including blood (serum lymphocytes), tumor tissue (vital snap frozen), adjacent normal epithelium. Patient data classification, molecular type, clinical outcome, results of model establishment are essential pillars. Overall,...
Summary The production of biodegradable polymers in transgenic plants is an important challenge plant biotechnology; nevertheless, it often accompanied by reduced fitness. In order to decrease the phenotypic abnormalities caused cytosolic polymer cyanophycin, and increase accumulation, four translocation pathway signal sequences for import into chloroplasts were individually fused coding region cyanophycin synthetase gene ( cph A Te ) Thermosynechococcus elongatus BP‐1, resulting constructs...
By a classical approach, the invasive margins of colorectal carcinomas can be typed as expansive or infiltrative, latter portending poor prognosis. Colorectal with infiltrative-type have long been known for their peculiar stroma and relative absence lymphohistiocytic host response, but they not studied contemporary gene expression techniques. Therefore, we submitted 18 microsatellite-stable that could having infiltrative (N = 7) type 11) confidence to nanoString nCounter® analysis (Tumor...
Zusammenfassung Wir berichten über einen nicht immunsupprimierten 60-jährigen Patienten mit mehrere Wochen progredienten, schmerzlosen subkutanen Noduli an der dominanten Hand bis Unterarm, die klinisch als Weichteiltumor vorrangig mesenchymaler Herkunft eingestuft wurden. Nach Exstirpation zweier Knötchen zur diagnostischen Abklärung zeigte Histologie eine chronisch-granulomatöse Entzündung Dermis und Subkutis zentralen, eosinophilen Nekrosen unter Einschluss von Zelldebris. Mittels...
Benign and malignant salivary gland tumours are clinically heterogeneous show different histology. Little is known about the role of human herpes virus 8 (HHV-8), Epstein-Barr (EBV), papillomavirus (HPV) infection in neoplasms. We investigated presence three viruses formalin-fixed, paraffin-embedded tissue samples a cohort 200 tumours. performed EBV-LMP-1 HHV-8 p16 immunohistochemistry, specific chip based hybridization assay for detection typing HPV chromogenic situ EBV analysis. Only one...
The production of biodegradable polymers that can be used to substitute petrochemical compounds in commercial products transgenic plants is an important challenge for plant biotechnology. Nevertheless, it often accompanied by reduced fitness. To decrease the phenotypic abnormalities sprout and increase polymer production, we restricted cyanophycin accumulation potato tubers using synthetase gene (cphA(Te)) from Thermosynechococcus elongatus BP-1, which under control tuber-specific class 1...
This study was designed to test programmed cell death 1 (PD-1) expression of T cells, the hallmark exhaustion, in different 'immune-classes' colorectal carcinoma microenvironments as delineated by unsupervised hierarchical cluster analysis.A tissue microarray made with punches from invasive margins 40 microsatellite-unstable and 34 microsatellite-stable carcinomas. Immune cells were phenotyped CD8, granzyme B, CD4, FoxP3, CD68, S-100, PD-1 ligand (PD-L1) immunohistochemistry; tumour area per...
Low-passage cancer cell lines are versatile tools to study tumor biology. Here, we have employed four such lines, established from primary tumors of colorectal (CRC) patients, evaluate effects the small molecule kinase inhibitors (SMI) vemurafenib, trametinib, perifosine, and regorafenib in an vitro setting. The mutant BRAF (V600E/V600K) inhibitor but also MEK1/2 trametinib efficiently inhibited DNA synthesis, signaling through ERK1/2 expression genes downstream cells only. In case AKT three...
Mice lines homozygous negative for one of the four DNA mismatch repair (MMR) genes (MLH1, MSH2, PMS2, MSH6) were generated as models MMR deficient (MMR-D) diseases. Clinically, hereditary forms MMR-D include Lynch syndrome (characterized by a germline gene defect) and constitutional MMR-D, biallelic form. knockout mice may be representative both Here, we aimed at characterizing MLH1-/- model focusing on tumor-immune microenvironment identification coding microsatellite mutations in lymphomas...
Tumour budding and podia formation are well-appreciated in surgical pathology as an aggressive invasion phenotype of colorectal carcinoma cells that is attained the microenvironment invasive margin. In this study, we addressed how tumour feature xenografts. Primary carcinomas (N = 44) various molecular types (sporadic standard type, high-degree microsatellite-unstable, CpG island methylator phenotype) were transplanted subcutaneously into T B cell-deficient NSG mice, making possible...
Malignant melanomas (MMs) are the abnormal proliferation of melanocytes and one lethal skin cancers in humans, equines, canines. Accordingly, MMs companion animals can serve as naturally occurring animal models, completing conventional cancer models. The common constitutive activation MAPK PI3K pathways has been described all three species. Targeting related is considered a potential option comparative oncologic approaches. Herein, we present cross-species analysis exposing set ten melanoma...
A substantial part of the human genome originates from transposable elements, remnants ancient retroviral infections. Roughly 8% consists about 400,000 LTR elements including endogenous retrovirus (HERV) sequences. Mainly, interplay between epigenetic and post-transcriptional mechanisms is thought to silence HERV expression in most physiological contexts. Interestingly, aberrant reactivation several HERV-H loci appears specific colorectal carcinoma (CRC).The Gag proteins (Gag-H) was assessed...
Infantile fibrosarcoma (IFS) is a rare pediatric tumor of intermediate malignancy with high local aggressiveness that typically presents in young infants. Its occurrence the head and neck region rare. Complete non-mutilating surgical resection often not possible, requiring multimodal treatment. IFS frequently harbors neurotrophic receptor tyrosine kinase (NTRK) fusions. Targeted therapy NTRK inhibitors modifying treatment paradigms IFS.
University and museum collections are very important sources of biological samples that can be used to asses the past present genetic diversity many species. Modern immunohistochemical techniques on long-term preserved fixed tissues from specimens answer epidemiological questions. A proof principle was established apply modern molecular genetics methods these old verify original diagnosis. We analysed 19 our university collection including human organs had been in fixative for more than 80...