A5026656382- Glycosylation and Glycoproteins Research
- Connective tissue disorders research
- Skin and Cellular Biology Research
- Clostridium difficile and Clostridium perfringens research
- Wnt/β-catenin signaling in development and cancer
- Probiotics and Fermented Foods
- Gut microbiota and health
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Polysaccharides and Plant Cell Walls
- Transgenic Plants and Applications
- Parathyroid Disorders and Treatments
- Hair Growth and Disorders
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Proteoglycans and glycosaminoglycans research
- Skin Protection and Aging
- Wound Healing and Treatments
- Pluripotent Stem Cells Research
- Bone and Dental Protein Studies
- Fibroblast Growth Factor Research
- Renal and related cancers
- Bacteriophages and microbial interactions
University of Tsukuba
2014-2022
National Institute of Advanced Industrial Science and Technology
2021-2022
The detailed mechanism of reprogramming somatic cells into induced pluripotent stem (iPSCs) remains largely unknown. Partially reprogrammed iPSCs are informative and useful for understanding the but remain technically difficult to generate in a predictable reproducible manner. Using replication-defective persistent Sendai virus (SeVdp) vectors, we analyzed effect decreasing expression levels OCT4, SOX2, KLF4, c-MYC found that low KLF4 reproducibly gives rise homogeneous population partially...
Bacterial glycans modulate the cross talk between gut microbiota and its host. However, little is known about these because of lack appropriate technology to study them. In this study, we applied Glycan-seq for glycan profiling intact mice. The evaluation cultured gram-positive (Deinococcus radiodurans) gram-negative (Escherichia coli) bacteria showed significantly distinct profiles bacteria, which were selected further analyzed by flow cytometry. results cytometry agreed well with those...
Aging in the epidermis is marked by a gradual decline barrier function, impaired wound healing, hair loss, and an increased risk of cancer. This could be due to age-related changes properties epidermal stem cells defective interactions with their microenvironment. Currently, no biochemical tools are available detect evaluate aging cells. The cellular glycosylation involved cell-cell communications cell-matrix adhesions various physiological pathological conditions. Here, we explored glycans...
Concanavalin A (ConA), a mannose (Man)-specific leguminous lectin isolated from the jack bean (Canavalia ensiformis) seed extracts, was discovered over century ago. Although ConA has been extensively applied in various life science research, recombinant mature expression not fully established. Here, we aimed to produce (rConA) lettuce (Lactuca sativa) using an Agrobacterium tumefaciens-mediated transient system. rConA could be produced as active form soluble fractions of leaves and purified...
Abstract Background There has been immense interest in studying the relationship between gut microbiota and human health. Bacterial glycans modulate cross talk its host. However, little is known about these because of lack appropriate technology to study them. Methods We previously developed a sequencing-based glycan profiling method called Glycan-seq, which based on use 39 DNA-barcoded lectins. In this study, we applied analyze glycome intact mice. Fecal was incubated with lectins exposed...
Abstract Tissue stem cells divide infrequently as a protective mechanism against internal and external stresses associated with aging. Here, we demonstrate that slow- fast-cycling interfollicular epidermal in mouse skin undergo distinct aging processes. Two years of lineage tracing reveals Dlx1+ slow-cycling clones expanded into the cell territory, while number Slc1a3+ gradually declined. Transcriptome analysis further indicated molecular properties each population are altered age. Mice...