A5034410817- Regulation of Appetite and Obesity
- Pharmacological Effects of Natural Compounds
- Diet and metabolism studies
- Gastrointestinal motility and disorders
- Diet, Metabolism, and Disease
- Neurobiology and Insect Physiology Research
- Biochemical effects in animals
- Fungal Biology and Applications
- Biochemical Analysis and Sensing Techniques
- Herbal Medicine Research Studies
- Apelin-related biomedical research
- Esophageal and GI Pathology
- Gastroesophageal reflux and treatments
- Neuropeptides and Animal Physiology
- bioluminescence and chemiluminescence research
- Ion Channels and Receptors
- Dysphagia Assessment and Management
- Growth Hormone and Insulin-like Growth Factors
- Morinda citrifolia extract uses
- Receptor Mechanisms and Signaling
- Gene Regulatory Network Analysis
- Exercise and Physiological Responses
- Fungal and yeast genetics research
- Pancreatic function and diabetes
- Mercury impact and mitigation studies
Erasmus University College Brussels
2022-2024
KU Leuven
1985-2022
Universitat Pompeu Fabra
2009-2010
Vlaams Instituut voor Biotechnologie
2006-2007
Universitat Autònoma de Barcelona
1995-1997
Mayo Clinic
1997
WinnMed
1997
University of Cagliari
1995
Directorate of Medicinal and Aromatic Plants Research
1989
Ghrelin, the endogenous ligand of growth hormone secretagogue receptor, is released from stomach. Animal studies suggest that ghrelin stimulates gastrointestinal motor activity.To investigate influence on gastric emptying rate and meal-related symptoms in idiopathic gastroparesis.In six patients with gastroparesis, a breath test was used to measure rates (t(1/2)) for solids liquids after administration saline or 40 microg/30 min double-blind, randomized fashion. At each sampling, patient...
High intrinsic basal signaling in mitogen-activated protein kinase pathways ensures proper dynamic responses to environmental stimuli.
The cAMP–PKA pathway consists of an extracellular ligand-sensitive G protein-coupled receptor, a protein signal transmitter, and the effector, adenylate cyclase, which product, cAMP, acts as intracellular second messenger. cAMP activates PKA by dissociating regulatory subunit from catalytic subunit. Yeast cells ( Saccharomyces cerevisiae ) contain glucose/sucrose-sensitive seven-transmembrane domain Gpr1, that was proposed to activate cyclase through α Gpa2. Consistently, we show here binds...
ERYTHROMYCIN A appears to be a motilin agonist, although (a peptide) and erythromycin 14‐member macrolide antibiotic carrying two sugar substituents) are structurally totally unrelated. To obtain information concerning the structural requirements for erythromycin's interaction with receptor, we studied 60 derivatives seven fragments of erythromycin, six macrolides 16‐member ring structure. For every compound ability displace bound crude homogenate rabbit antral smooth muscle tissue induce...
Whole-cell biosensors could serve as eco-friendly and cost-effective alternatives for detecting potentially toxic bioavailable heavy metals in aquatic environments. However, they often fail to meet practical requirements due an insufficient limit of detection (LOD) high background noise. In this study, we designed a synthetic genetic circuit specifically tailored ionic mercury, which applied environmental samples collected from artisanal gold mining sites Peru. We developed two distinct...
Motilin is a potent agonist for gastrointestinal smooth muscle contraction and has been proposed to regulate the onset of phase III migrating motor complex in dogs humans. The effects motilin OHM-11526, antagonist rabbit strips, were examined isolated canine human jejunal circular cells using whole cell patch-clamp techniques with Ba2+ as charge carrier. Effects both drugs on inward current through L-type Ca2+ channels (ICaL) first observed at 10(-3) M. At 10(-6) M, increased ICaL by 43 +/-...
The maternal and fetal endocrine pancreas were investigated in the diabetic BB rat on day 21 of pregnancy. contained practically no insulin-positive B cells. A D cell mass also decreased, while plasma glucagon somatostatin levels normal or increased, confirming previous data. Six 11 rats had B-cell-specific surface antibodies (ICSA), whereas only 1 10 nondiabetic was ICSA-positive. volume density cells decreased compared to fetuses rats, but glucagon- somatostatin-positive remained normal....
This is the first report on central motilin receptors. Autoradiography cerebellar slices revealed specific motilin-binding sites in molecular layer of cortex. Scatchard analysis cold saturation studies showed existence a high-(pKd,hi = 9.07 +/- 0.09, where pKd negative logarithm dissociation constant) and low-affinity binding site (pKd,lo 6.56 0.09). Similar affinities were found with rabbit porcine (po) antagonist [Phe3, Leu13]po-motilin. Feline canine had markedly lower affinity for 5.29...
Juvenile hormone epoxide hydrolase (JHEH) plays an important role in the metabolism of JH III insects. To study control JHEH female Drosophila melanogaster, 1, 2 and 3 cDNAs were cloned sequenced. Northern blot analyses showed that three transcripts are expressed head thorax, gut, ovaries fat body females. Molecular modeling shows enzyme is a homodimer binds juvenile acid (JH IIIA) at catalytic groove better than III. Analyses promoters expressing short promoter sequences behind reporter...
Rhythmic oscillating complex (ROC) is a highly organized gastrointestinal motility pattern recently described in fasted avian species. ROCs show several high-speed aborad-propagated contractions that progressively change into others of orad direction. In addition, chickens migrating motor complexes (MMC) both fed and fasting states. Recently, motilin was isolated characterized from chicken small intestine. Accordingly, the aim this study to learn whether might be involved either ROC or MMC...
Juvenile hormone epoxide hydrolase (JHEH) plays an important role in the metabolism of juvenile III (JH III) insects. To study that JHEH female Aedes aegypti 1, 2, and 3 complementary DNA (cDNAs) were cloned sequenced. Northern blot analyses show three transcripts are expressed head thorax, gut, ovaries, fat body females. Molecular modeling shows enzyme is a homodimer binds JH acid IIIA) at catalytic groove better than III. The cDNA 1 2 very similar indicating close relationship. Knocking...