A5045635519- RNA modifications and cancer
- Biochemical and Molecular Research
- Chemotherapy-induced cardiotoxicity and mitigation
- Paraoxonase enzyme and polymorphisms
- Reproductive System and Pregnancy
- Chemotherapy-induced organ toxicity mitigation
- Pregnancy and preeclampsia studies
- PARP inhibition in cancer therapy
- CRISPR and Genetic Engineering
- Allergic Rhinitis and Sensitization
- Peptidase Inhibition and Analysis
- Cancer-related gene regulation
- Cancer-related Molecular Pathways
- Sirtuins and Resveratrol in Medicine
- CAR-T cell therapy research
- Renal cell carcinoma treatment
- Genomics, phytochemicals, and oxidative stress
- Cancer Research and Treatments
- Autoimmune Bullous Skin Diseases
- Cynara cardunculus studies
- Blood transfusion and management
- Nonmelanoma Skin Cancer Studies
- Oral Health Pathology and Treatment
- Autophagy in Disease and Therapy
- Cancer Cells and Metastasis
Marche Polytechnic University
2016-2025
New York Structural Biology Center
2016-2018
Ospedali Riuniti di Ancona
2016
Fondazione Salvatore Maugeri
2000-2004
Istituti di Ricovero e Cura a Carattere Scientifico
2000-2002
Sapienza University of Rome
1986
Background Improved detection methods for diagnosis of malignant pleural mesothelioma (MPM) are essential early and reliable as well treatment. Since recent data point to abnormal levels microRNAs (miRNAs) in tumors, we hypothesized that a profile deregulated miRNAs may be marker MPM the specific used monitoring its progress. Methods Results isolated from fresh-frozen biopsies patients were tested expression 88 types miRNA involved cancerogenesis. Most downregulated tissues compared with...
Nicotinamide N-methyltransferase (NNMT) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl-l-methionine as donor. NNMT plays a significant role in regulation metabolic pathways is expressed at markedly high levels several kinds cancers, presenting it potential molecular target for cancer therapy. We have determined crystal structure human ternary complex bound to both demethylated donor S-adenosyl-l-homocysteine acceptor substrate 2.7 Å resolution....
Background/Aims: Head and neck squamous cell carcinoma (HNSCC) ranks sixth worldwide for tumor-related mortality. A subpopulation of tumor cells, termed cancer stem cells (CSCs), has the ability to support growth. Therefore, profiling CSC-enriched populations could be a reliable tool study biology. Methods: We performed phenotypic characterization 7 HNSCC lines evaluated presence CSCs. CSCs from Hep-2 line primary cultures were enriched through sphere formation sphere-forming have been...
Melanoma represents the most aggressive skin cancer, being responsible for majority of deaths related with these neoplasms. Despite chemotherapy a frontline approach management advanced stages disease, it displayed poor response rates and short-term efficacy due to melanoma cell resistance. Therefore, discovery molecules that can be used effective targeted therapy is crucial. In this study, we evaluated impact paraoxonase-2 (PON2) silencing on proliferation, viability, resistance treatment...
Melanoma accounts for less than 5% of all cutaneous neoplasms but is responsible the greater part skin cancer-related deaths. Therefore, identification molecules that could serve as therapeutic target urgent. This study focused on enzyme nicotinamide N-methyltransferase (NNMT). The effect NNMT knockdown cell proliferation and migration A375 melanoma cells was evaluated by MTT wound healing assays, respectively. Viability downregulating also explored under treatment with dacarbazine, a...
Oral squamous cell carcinoma represents the most aggressive and frequent form of head neck cancer. Due to drug resistance, 5-year survival rate patients with advanced disease is less than 50%. In order identify molecular targets for effective oral cancer treatment, we focused on paraoxonase-2 enzyme. Indeed, based data previously obtained from preliminary immunohistochemistry Western blot analyses performed tissue specimens, enzyme was found be upregulated in tumor compared normal mucosa....
Oral squamous cell carcinoma (OSCC) is the most common type of oral cancer. Despite progress in treatment OSCC, overall survival has not improved substantially last three decades. Therefore, identification reliable biomarkers becomes essential to develop effective anti-cancer therapy. In this study, we focused on enzyme Nicotinamide N-methyltransferase (NNMT), which plays a fundamental role biotransformation many xenobiotics. Although several tumors have been associated with abnormal NNMT...
Paraoxonase-2 (PON2) is a ubiquitously expressed intracellular protein that localized in the perinuclear region, endoplasmic reticulum (ER), and mitochondria, also associated with plasma membrane. PON2 functions as an antioxidant enzyme by reducing levels of reactive oxygen species (ROS) mitochondria ER through different mechanisms, thus having anti-apoptotic effect preventing formation atherosclerotic lesions. While antiatherogenic role played this has been extensively explored within...
Abstract Background Osteosarcoma (OS) is a primary bone malignancy that mostly affects young people, characterized by high metastatic potential, and marked chemoresistance responsible for disease relapse in most patients. Therefore, it necessary to identify novel molecules setup targeted strategies improve the clinical outcome. The enzyme nicotinamide N‐methyltransferase (NNMT) catalyses N‐methylation of other analogs, playing crucial role biotransformation drugs xenobiotics. NNMT...
Abstract Merkel cell carcinoma (MCC) is an aggressive skin cancer, with a propensity for early metastasis. Therefore, diagnosis and the identification of novel targets become fundamental. The enzyme nicotinamide N -methyltransferase (NNMT) catalyzes reaction -methylation other analogous compounds. Although NNMT overexpression was reported in many malignancies, significance its dysregulation cancer phenotype partly clarified. Several works demonstrated that promotes proliferation, migration,...
We investigated expression levels of Nicotinamide N-Methyltransferase (NNMT), an enzyme involved in the biotransformation many drugs and xenobiotic compounds, oral squamous cell carcinoma (OSCC). Measurements were performed by immunohistochemistry relationship between tumour characteristics NNMT OSCC was studied to evaluate effectiveness as a prognostic marker cavity. In conclusion, present study suggests that may have potential biomarker therapeutic target for OSCC.
Oral squamous cell carcinoma (OSCC) is the most common form of head and neck cancer worldwide. In recent decades, 5-year mortality rate approximately 50% around world. As reliable biomarkers oral are still lacking, it necessary to identify new target molecules for early diagnosis, effective therapy, monitoring disease. present work, we focused on expression enzyme nicotinamide N-methyltransferase (NNMT). We analyzed activity in 37 paired tumor non-tumor tissues found that levels...
Lung cancer is the second most commonly diagnosed neoplasm, and represents leading cause of tumour death worldwide. As patients are often at a late stage, current therapeutic strategies have limited effectiveness prognosis remains poor. Successful treatment depends on early diagnosis knowledge concerning molecular mechanisms underlying lung carcinogenesis. In present study, we focused nicotinamide N-methyltransferase (NNMT), which overexpressed in several malignancies. First, analysed NNMT...
Nicotinamide N -methyltransferase (NNMT) is an enzyme that catalyzes the -methylation of nicotinamide and pyridine compounds, participating in xenobiotic drug metabolism. Data on literature have evidenced a possible role NNMT many solid cancers, but no data are currently available cutaneous melanoma. Recent important advances been achieved treatment advanced melanoma with targeted therapy immunotherapy. However, identification biomarkers can be used for detection early stage disease as well...
The goal of the current study was to identify potential roles paraoxonase-2 in bladder carcinogenesis. T24 cancer cells were transfected with plasmids inducing silencing or overexpression. Upon selection clones stably down- upregulating paraoxonase-2, cell proliferation, migration, and production reactive oxygen species evaluated, before after treatment cisplatin gemcitabine, used alone combination. activity levels both caspase-3 caspase-8 also analyzed. shRNA-mediated gene overexpression...
Abstract The cancer stem cell theory states that a subset of tumor cells, termed cells (CSCs), has the ability to self‐renew and differentiate within tumors. According this theory, CSCs would be mainly responsible for initiation, progression, resistance therapy, recurrence, metastasis. In study, culture system was setup enrich from bladder (T24), lung (A549), colorectal (CaCo‐2), osteosarcoma (MG63) lines, through sphere formation. Magnetic‐activated sorting also used further increase CSC...