A5074774989- Enzyme-mediated dye degradation
- Lignin and Wood Chemistry
- Metal-Catalyzed Oxygenation Mechanisms
- Biochemical and biochemical processes
- Biofuel production and bioconversion
- Enzyme Structure and Function
- Biochemical and Molecular Research
- Microbial Metabolic Engineering and Bioproduction
- Carbohydrate Chemistry and Synthesis
- Bacterial Genetics and Biotechnology
- Enzyme Catalysis and Immobilization
- Bacteriophages and microbial interactions
- Microbial bioremediation and biosurfactants
- Amino Acid Enzymes and Metabolism
- RNA and protein synthesis mechanisms
- Cancer therapeutics and mechanisms
- Microbial Metabolism and Applications
- Microbial metabolism and enzyme function
- Chemical Synthesis and Analysis
- Porphyrin Metabolism and Disorders
- Metabolism and Genetic Disorders
- Glycosylation and Glycoproteins Research
- Plant Parasitism and Resistance
- Plant biochemistry and biosynthesis
- Biochemical Acid Research Studies
University of Warwick
2016-2025
Coventry (United Kingdom)
2015-2021
University of Illinois Urbana-Champaign
2008
University of Leeds
2008
University of Southampton
1993-2006
Queen's University Belfast
2006
Iwate University
2003
Tenovus Cancer Care
2003
Syngenta (United Kingdom)
2001
Institut Pasteur
1992
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMolecular basis for vancomycin resistance in Enterococcus faecium BM4147: biosynthesis of a depsipeptide peptidoglycan precursor by proteins VanH and VanATimothy D. H. Bugg, Gerard Wright, Sylvie Dutka-Malen, Michel Arthur, Patrice Courvalin, Christopher T. WalshCite this: Biochemistry 1991, 30, 43, 10408–10415Publication Date (Print):October 1, 1991Publication History Published online1 May 2002Published inissue 1 October...
Rhodococcus jostii RHA1, a polychlorinated biphenyl-degrading soil bacterium whose genome has been sequenced, shows lignin degrading activity in two recently developed spectrophotometric assays. Bioinformatic analysis reveals unannotated peroxidase genes present the of R. RHA1 with sequence similarity to open reading frames other lignin-degrading microbes. They are members Dyp family and were annotated as DypA DypB, on basis bioinformatic analysis. Assay gene deletion mutants using...
Significance Metabolism of l -carnitine, a compound abundant in human diet, to trimethylamine by microbiota has been shown promote atherosclerosis and subsequent development heart disease. However, the underpinning molecular biochemical mechanisms remain unknown. In this study, we reveal that previously unidentified Rieske-type protein is responsible for carnitine transformation from microbiota. Knowledge gained our study provides opportunity not only explore Rieske inhibitors preventing...
Two spectrophotometric assays have been developed to monitor breakdown of the lignin component plant lignocellulose: a continuous fluorescent assay involving fluorescently modified lignin, and UV-vis chemically nitrated lignin. These used analyse degradation activity in bacterial fungal degraders, identify additional soil bacteria that show for degradation. known act as aromatic Pseudomonas putida Rhodococcus sp. RHA1, consistently showed these assays, strains were shown small scale...
The aromatic polymer lignin represents a possible renewable source of chemicals, if biocatalytic routes for breakdown can be developed. availability genome sequence Rhodococcus jostii RHA1, bacterium that breaks down lignin, has allowed the application targeted pathway engineering strategy to produce vanillin, valuable food/flavor chemical. A gene deletion strain R. RHA1 in which vanillin dehydrogenase had been deleted, when grown on minimal medium containing 2.5% wheat straw lignocellulose...
• Strigolactones are considered a novel class of plant hormones that, in addition to their endogenous signalling function, exuded into the rhizosphere acting as signal stimulate hyphal branching arbuscular mycorrhizal (AM) fungi and germination root parasitic seeds. Considering importance strigolactones biosynthetic origin (from carotenoids), we investigated relationship with hormone abscisic acid (ABA). Strigolactone production ABA content presence specific inhibitors oxidative carotenoid...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIdentification of vancomycin resistance protein VanA as a D-alanine:D-alanine ligase altered substrate specificityTimothy D. H. Bugg, Sylvie Dutka-Malen, Michel Arthur, Patrice Courvalin, and Christopher T. WalshCite this: Biochemistry 1991, 30, 8, 2017–2021Publication Date (Print):February 1, 1991Publication History Published online1 May 2002Published inissue 1 February...
Using a continuous fluorescence-based enzyme assay, we have characterized the antibacterial agents tumicamycin and liposidomycin B as inhibitors of solubilized Escherichia coli phospho-N-acetylmuramyl-pentapeptide translocase. Tunicamycin exhibited reversible inhibition (Ki = 0.55 +/- 0.1 microM) which was noncompetitive with respect to lipid acceptor substrate competitive fluorescent analog, dansyl-UDPMurNAc-pentapeptide. Liposidomycin slow-binding 80 15 nM) These results provide insight...
To develop a method to detect bacteria from environmental samples that are able metabolize lignin.A previously developed UV-vis assay for lignin degradation activity has been use as spray on agar plates. Nine mesophilic strains were isolated using this woodland soil incubated in enrichment cultures containing wheat straw lignocellulose: four Microbacterium isolates, two Micrococcus Rhodococcus erythropolis (all Actinobacteria) and Ochrobactrum isolates (Alphaproteobacteria). Three...
The soil bacterium Rhodococcus jostii RHA1 contains two dye-decolorizing peroxidases (DyPs) named according to the subfamily they represent: DypA, predicted be periplasmic, and DypB, implicated in lignin degradation. Steady-state kinetic studies of these enzymes revealed that have much lower peroxidase activities than C- D-type DyPs. Nevertheless, DypA showed 6-fold greater apparent specificity for anthraquinone dye Reactive Blue 4 (k(cat)/K(m) = 12800 ± 600 M(-1) s(-1)) either ABTS or...
A gene insertion approach is used in <italic>Rhodococcus jostii</italic> RHA1 to generate pyridine-dicarboxylic acid bioproducts from lignin.