A5056778513- Adenosine and Purinergic Signaling
- BIM and Construction Integration
- Architecture and Computational Design
- Retinal Diseases and Treatments
- Calcium signaling and nucleotide metabolism
- Oral microbiology and periodontitis research
- Neuroscience of respiration and sleep
- Plant Molecular Biology Research
- Modular Robots and Swarm Intelligence
- Innovations in Concrete and Construction Materials
- Connexins and lens biology
- Wound Healing and Treatments
- Light effects on plants
- FOXO transcription factor regulation
- Photosynthetic Processes and Mechanisms
- Retinal Development and Disorders
- Retinopathy of Prematurity Studies
- Neuroscience and Neuropharmacology Research
- Construction Project Management and Performance
- Inflammasome and immune disorders
- Fibroblast Growth Factor Research
- Mesenchymal stem cell research
- Occupational Health and Safety Management
- Bone Metabolism and Diseases
- Surgical Simulation and Training
University of California, San Diego
2023-2024
Universitas Pramita Indonesia
2020-2023
University of Nevada, Reno
2007-2022
Northwestern University
2022
Englewood Hospital and Medical Center
2022
Tarumanagara University
2019-2020
Eastman Dental Hospital
2019
University College London
2019
University of Pennsylvania
2009-2018
Agency for Science, Technology and Research
2011-2015
Plastid-to-nucleus retrograde signaling coordinates nuclear gene expression with chloroplast function and is essential for the photoautotrophic life-style of plants. Three signals have been described, but little known their pathways. We show here that GUN1, a chloroplast-localized pentatricopeptide-repeat protein, ABI4, an Apetala 2 (AP2)–type transcription factor, are common to all three ABI4 binds promoter retrograde-regulated through conserved motif found in close proximity...
Plastid-to-nucleus retrograde signaling coordinates nuclear gene expression with chloroplast function and is essential for the photoautotrophic life-style of plants. Three signals have been described, but little known their pathways. We show here that GUN1, a chloroplast-localized pentatricopeptide-repeat protein, ABI4, an Apetala 2 (AP2)–type transcription factor, are common to all three ABI4 binds promoter retrograde-regulated through conserved motif found in close proximity...
Mechanical deformation produces complex effects on neuronal systems, some of which can lead to dysfunction and death. While astrocytes are known respond mechanical forces, it is not clear whether neurons also directly. We examined mechanosensitive ATP release the physiological response this in isolated retinal ganglion cells. Purified cells released upon swelling. Release was blocked by carbenoxolone, probenecid or peptide (10)panx, implicating pannexin channels as conduits. stretch...
As adenosine 5′‐triphosphate (ATP) released from astrocytes can modulate many neural signaling systems, the triggers and pathways for this ATP release are important. Here, ability of mechanical strain to trigger through pannexin channels effects sustained on expression were examined in rat optic nerve head astrocytes. Astrocytes when subjected 5% equibiaxial or hypotonic swelling. Although expressed mRNA pannexins 1–3, connexin 43, VNUT, pharmacological analysis suggested a predominant role...
Inflammatory responses play a key role in many neural pathologies, with localized signaling from the non-immune cells making critical contributions. The NLRP3 inflammasome is an important component of innate immune and can link insult to chronic inflammation. requires two stages contribute: priming activation. stage involves upregulation components while activation results assembly complex. step be rate limiting connect inflammation, but our knowledge signals that regulate sterile...
Lysosomal enzymes function optimally in acidic environments, and elevation of lysosomal pH can impede their ability to degrade material delivered lysosomes through autophagy or phagocytosis. We hypothesize that abnormal is a key aspect diseases accumulation restoring will improve cell function. The propensity nanoparticles end up the lysosome makes them an ideal method delivering drugs lysosomes. This study asked whether could traffic lysosomes, lower enhance degradation by cultured human...
Abstract Bacterial pathogens stimulate periodontitis, the most common osteolytic disease in humans and cause of tooth loss adults. Previous studies identified leukocytes their products as key factors this process. We demonstrate for first time that osteoblast lineage cells play a critical role periodontal disease. Oral infection stimulated nuclear localization NF-κB osteoblasts osteocytes periodontium wild type but not transgenic mice expressed specific dominant negative mutant IKK (IKK-DN)...
Lysosomal enzymes function optimally at low pH; as accumulation of waste material contributes to cell aging and disease, dysregulation lysosomal pH may represent an early step in several pathologies. Here, we demonstrate that stimulation the P2X7 receptor (P2X7R) for ATP alkalinizes lysosomes cultured human retinal pigmented epithelial (RPE) cells impairs function. P2X7R did not kill RPE but alkalinized by 0.3 U. Receptor also elevated cytoplasmic Ca2+; Ca2+ influx was necessary sufficient...
Healing is delayed in diabetic wounds. We previously demonstrated that lineage-specific Foxo1 deletion keratinocytes interfered with normal wound healing and keratinocyte migration. Surprisingly, the same of wounds had opposite effect, significantly improving response. In glucose media, forkhead box O1 (FOXO1) enhanced migration through up-regulating TGFβ1. high glucose, FOXO1 nuclear localization was induced but did not bind to TGFβ1 promoter or stimulate transcription. Instead, expression...
Positron emission tomography (PET) image quantification is a challenging problem due to limited spatial resolution of acquired data and the resulting partial volume effects (PVE), which depend on size structure studied in relation may lead over or underestimation true tissue tracer concentration. In addition, it usually necessary perform smoothing either during reconstruction afterwards achieve reasonable signal-to-noise ratio. Typically, an isotropic Gaussian filtering (GF) used for this...
Mechanical strain due to increased pressure or swelling activates inflammatory responses in many neural systems. As cytokines and chemokine messengers lead both pro-inflammatory neuroprotective actions, understanding the signaling patterns triggered by mechanical stress may help improve overall outcomes. While cytokine systems is often associated with glial cells like astrocytes microglia, contribution of neurons themselves response underappreciated has bearing on any balanced response....
Abstract Mechanical strain in neural tissues can lead to the up‐regulation and release of multiple cytokines including interleukin 6 (IL‐6). In retina, mechanosensitive ATP autostimulate P2X7 receptors on both retinal ganglion cell neurons optic nerve head astrocytes. Here, we asked whether purinergic signaling contributed IL‐6 response increased intraocular pressure (IOP) vivo , stretch or swelling vitro . Rat mouse eyes were exposed non‐ischemic elevations IOP 50–60 mmHg for 4 h. A PCR...
The transient receptor potential cation channel mucolipin 1 (TRPML1) is a conduit for lysosomal calcium efflux, and activity may be affected by contents. lysosomes of retinal pigmented epithelial (RPE) cells are particularly susceptible to build-up waste products because they must degrade the outer segments phagocytosed daily from adjacent photoreceptors; incomplete degradation leads accumulation lipid in lysosomes. This study asks whether stimulation TRPML1 can release RPE such...
Purpose All industry has been facing tremendous pressure since the beginning of 2020 owing to COVID-19 crisis, including real estate construction. The research is a case study investigating challenges and action plans faced by stakeholders (developers/consultants/contractors) in construction some major cities Indonesia. This aims identify prioritize on related stakeholders. Design/methodology/approach gathered distributing open questionnaire expert get insights their own verbatim. Data...
Background: Diabetes is known to impair wound healing and deteriorate the periodontal condition. There limited information about patterns events associated with repair. In this study, we evaluate dynamics of repair using micro‐computed tomography (microCT) immunohistochemistry. Methods: Thirty‐six male rats were used, diabetes was induced by streptozotocin. The maxillary first molars extracted, a tooth‐associated osseous defect created in extraction area. Animals sacrificed after 7, 14, 21...
Re-epithelialization is an important part in mucosal wound healing. Surprisingly little known about the impact of diabetes on molecular events We examined role transcription factor forkhead box O1 (Foxo1) oral wounds diabetic and normoglycemic mice with keratinocyte-specific Foxo1 deletion. Diabetic had significantly delayed healing reduced cell migration proliferation. deletion rescued negative but opposite effect mice. Diabetes vivo high glucose conditions vitro enhanced expression...
Keratinocyte migration is a key aspect of re-epithelialization during wound healing. Matrix metalloproteinase 9 (MMP9) contributes to this process and deficiencies in the MMP9 lead impaired Inappropriate expression also re-epithelialization. Previously we demonstrated that FOXO1 was activated healing but higher levels diabetic wounds. To address mechanisms examined vivo full thickness dermal scalp wounds created experimental K14.Cre + .Foxo1 L/L mice with lineage-specific Cre recombinase...
Cross-reactions between innate immunity, lysosomal function, and purinergic pathways may link signaling systems in cellular pathologies. We found activation of toll-like receptor 3 (TLR3) triggers ATP release from both astrocytes retinal pigmented epithelial (RPE) cells. efflux was accompanied by acid phosphatase beta hexosaminidase release. Poly(I:C) alkalinized lysosomes, alkalization with bafilomycin or chloroquine triggered Lysosomal rupture glycyl-L-phenylalanine-2-naphthylamide (GPN)...