A5044207292- Polar Research and Ecology
- Microbial Community Ecology and Physiology
- Algal biology and biofuel production
- Biocrusts and Microbial Ecology
- Protist diversity and phylogeny
- Marine and coastal ecosystems
- Aquatic Ecosystems and Phytoplankton Dynamics
- Diatoms and Algae Research
- Marine and coastal plant biology
- Mass Spectrometry Techniques and Applications
- Plant Pathogens and Fungal Diseases
- Wastewater Treatment and Nitrogen Removal
- Plant and Fungal Species Descriptions
- Metabolomics and Mass Spectrometry Studies
- Soil and Water Nutrient Dynamics
- Water Quality and Pollution Assessment
- Food Industry and Aquatic Biology
- Fungal and yeast genetics research
- Winter Sports Injuries and Performance
- Analytical chemistry methods development
- Inflammatory Bowel Disease
- Yeasts and Rust Fungi Studies
- Photosynthetic Processes and Mechanisms
- Plant and fungal interactions
- Geology and Paleoclimatology Research
Charles University
2016-2025
Institute of Botany of the Slovak Academy of Sciences
2025
University of Salzburg
2024
University of Applied Sciences Upper Austria
2024
Czech Academy of Sciences, Institute of Botany
1983-2023
Czech Academy of Sciences
2022-2023
Iscare I.V.F
2020
University of Chemistry and Technology, Prague
2013-2017
Hydrobiological Institute
1970-1993
Institute of Landscape Ecology of the Slovak Academy of Sciences
1983
Click to increase image sizeClick decrease size This article is part of the following collections: 1922–2022: 100 years SIL publications
Melting snowfields in polar and alpine regions often exhibit a red orange colouration caused by microalgae. The diversity of these organisms is still poorly understood. We applied polyphasic approach using three molecular markers light electron microscopy to investigate spherical cysts sampled from mountains Europe, North America South as well both regions. Molecular analyses revealed the presence single independent lineage within Chlamydomonadales. genus Sanguina described, with nivaloides...
Melting snow fields populated by aplanozygotes of the genus Chloromonas (Chlamydomonadales, Chlorophyta) are found in polar and alpine habitats.In High Tatra Mountains (Slovakia), cells causing blooms brownish-red designated as Scotiella tatrae Kol turned out to be genetically (18S, ITS1 ITS2 rDNA, rbcL) very closely related nivalis (Chodat) Hoham et Mullet from Austrian Alps.Therefore, Sc. is transferred into latter taxon reduced a subspecies Cr.nivalis subsp.tatrae.Both exhibit similar...
Melting polar and alpine ice surfaces frequently exhibit blooms of dark pigmented algae. These microbial extremophiles significantly reduce the surface albedo glaciers, thus accelerating melt rates. However, ecology, physiology taxonomy cryoflora are not yet fully understood. Here, a Swiss an Austrian glacier dominated either by filamentous
The uptake of ammonia and nitrate by particulate matter during photosynthesis in large light dark bottles was followed colorimetric mass spectrometric methods. Ammonia as determined the 15 N isotope always higher than parallel estimate from analysis. (or release) inversely related to protein content corresponding sample matter. Utilization proportional concentration available. organic contained 50.5% but 61.7% ones. mean ratio oxygen changes, calculated changes protein, carbohydrate, lipid...
Abstract Melting glacier surfaces are unique ecosystems for specialized microbes, frequently harbouring blooms of microalgae with pigments contributing to the darkening ice surfaces, reducing albedo and enhancing melt rates. The main cause this phenomenon is algae genus Ancylonema . Prior investigation depended on field-collected material because these resisted cultivation. To enhance research how dominate melting ice, we established a strain alaskanum from an alpine exposed temperatures...
Abstract Melting snow and glacier surfaces host microalgal blooms in polar mountainous regions. The aim of this study was to determine the dominant taxa at species level European Arctic Alps. A standardized protocol for amplicon metabarcoding using 18S rRNA gene ITS2 markers developed. This is important because previous biodiversity studies have been hampered by dominance closely related algal ice. Due limited resolution partial Illumina sequences, hypervariable region used further...
Ice-binding proteins (IBPs) of the DUF3494 type have been found in many ice-associated unicellular photoautotrophs, including chlorophytes, haptophytes, diatoms and a cyanobacterium. Unrelated IBPs land plants (streptophytes). Here we looked for two streptophyte algae that grow only on glaciers, group which not previously examined. The species, Ancylonema nordenskioeldii Ancylonema. alaskanum, belong to class Zygnematophyceae, whose members are closest relatives all plants. We one them, A....
Glacier ice algae and snow are important primary producers in extreme cold environments significantly impact global processes through their role carbon cycling glacier melting by reducing surface albedo. These organisms have evolved unique cold-adaptation mechanisms, making them promising candidates for biotechnological applications, yet few species characterised detail. Such studies also becoming particularly urgent, as polar cryospheric habitats rapidly changing because of the ongoing...
Snow algae (SA) significantly influence snowmelt dynamics and biogeochemical cycles by reducing snow albedo modulating concentration of some ions, thereby accelerating melt. Conversely, SA proliferate during when sufficient liquid water is present in the snowpack, adequate solar radiation fuels photosynthesis. This study investigates diversity with focus on forest species their role hydrological regimes within forested high mountain regions examines connection to climate change impacts.We...
Snow algae are excellent models for elucidating adaptations to abiotic stresses that characterize their extreme habitat. In cold-adapted microorganisms, changes in lipid composition represent an important strategy enables survival at low temperatures. However, our knowledge this field remains fragmentary. Using shotgun lipidomics, we identified 303 species samples of snow algal blooms originating from 14 sites across a wide altitudinal gradient mountains Central Europe. Red, orange, and...
Based on analyses of multiple molecular markers (18S rDNA, ITS1, ITS2
Abstract Snow algae blooms often form green or red coloured patches in melting alpine and polar snowfields worldwide, yet little is known about their biology, biogeography, species diversity. We investigated eight isolates collected from snow northern Norway, using a combination of morphology, 18S rRNA gene internal transcribed spacer 2 (ITS2) genetic markers. Phylogenetic ITS2 secondary structure analyses assigned six to the Raphidonema nivale, Deuterostichococcus epilithicus, Chloromonas...
<title>Abstract</title> Psychrophilic pucciniomycotinous yeasts inhabit snowfields and glacial ecosystems worldwide, yet their ecological role remains unclear. We isolated a clonal strain of Phenoliferia psychrophenolica (LCC-F-001-001) from an alpine red snowfield. Its 42-Mbp genome contains 11,523 genes, including 37 ice-binding protein the highest number recorded in fungi, mainly acquired through horizontal transfers. This yeast tolerates freezing, grows optimally at 10°C forms...
Here, we report an effect of short acclimation to a wide span temperatures on photosynthetic electron transfer, lipid and fatty acid composition in the snow alga Chlamydomonas cf. nivalis. The growth oxygen evolution capacity were low at 2 °C yet progressively enhanced 10 significantly higher from 5 15 comparison with mesophilic control reinhardtii. In search molecular mechanisms responsible for adaptation photosynthesis temperatures, have found unprecedented high rates QA QB transfer....
Slowly melting snowfields in mountain and polar regions are habitats of snow algae. Orange blooms were sampled three European ranges. The cysts within the morphologically resembled those Chloromonas nivalis (Chlorophyceae). Molecular morphological traits field cultured material showed that they represent a new species, hindakii sp. nov. performance photosystem II was evaluated by fluorometry. For first time for alga, cyst stages collected wide altitudinal gradient laboratory strain compared....
The determination of polyvinylalcohol (PVA) by the formation blue iodine–PVA complex method was established for a semi-micro scale. direct addition iodine solution proved to be better source than generated in situ. ageing reagents requires frequent calibrations. Common biopolymers such as starch, albumin and cellulose produced much lower colour intensity with their impact on analysis is negligible. not affected paper filter filtration. detection PVA successfully tested different wastewater...
Melting snowfields are dominated by closely related green algae.Although microscopy-based classification evaluable distinction tools, they can be challenging and may not reveal the diversity.High-throughput sequencing (HTS) allows for a comprehensive community evaluation but has been rarely used in such ecosystems.We found that assigning taxonomy to DNA sequences strongly depends on quality of reference databases.Furthermore, an accurate identification, combination manual inspection...