A5039626692- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- Sleep and Wakefulness Research
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Crystallography and molecular interactions
- EEG and Brain-Computer Interfaces
- Anesthesia and Sedative Agents
- Zebrafish Biomedical Research Applications
- Photoreceptor and optogenetics research
- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Epilepsy research and treatment
- Asymmetric Synthesis and Catalysis
- Asymmetric Hydrogenation and Catalysis
- Scientific Computing and Data Management
- Molecular spectroscopy and chirality
- Anesthesia and Neurotoxicity Research
University of California, San Francisco
2018-2024
University of California, Berkeley
2024
University of San Francisco
2024
Massachusetts General Hospital
2016-2021
Massachusetts Institute of Technology
2016-2019
Harvard University
2016
Dopamine (DA) promotes wakefulness, and DA transporter inhibitors such as dextroamphetamine methylphenidate are effective for increasing arousal inducing reanimation, or active emergence from general anesthesia. neurons in the ventral tegmental area (VTA) involved reward processing, motivation, emotion, reinforcement, cognition, but their role regulating wakefulness is less clear. The current study was performed to test hypothesis that selective optogenetic activation of VTA sufficient...
Abstract The hippocampus is a mammalian brain structure that expresses spatial representations 1 and crucial for navigation 2,3 . Navigation, in turn, intricately depends on locomotion; however, current accounts suggest dissociation between hippocampal the details of locomotor processes. Specifically, thought to represent mainly higher-order cognitive variables such as position, speed direction movement 4–7 , whereas limb movements propel animal can be computed represented primarily...
Objective. Personalized automatic control of medically-induced coma, a critical multi-day therapy in the intensive care unit, could greatly benefit clinical and further provide novel scientific tool for investigating how brain response to anesthetic infusion rate changes during therapy. would require real-time tracking inter- intra-subject variabilities while simultaneously delivering therapy, which has not been achieved. Current systems coma separate offline model fitting experiment deal...
Sigmatropic rearrangements number among the most powerful complexity-building transformations in organic synthesis but have remained largely insensitive to enantioselective catalysis due diffuse nature of their transition structures. Here, we describe a synergistic ion-binding strategy for asymmetric anionic sigmatropic rearrangements. This approach is demonstrated with [2,3]-Wittig rearrangement α-allyloxy carbonyl compounds afford highly enantioenriched homoallylic alcohol products. Chiral...
Although general anesthetics are routinely administered to surgical patients induce loss of consciousness, the mechanisms underlying anesthetic-induced unconsciousness not fully understood. In rats, we characterized changes in extradural EEG and intracranial local field potentials (LFPs) within prefrontal cortex (PFC), parietal (PC), central thalamus (CT) response progressively higher doses inhaled anesthetic sevoflurane. During induction with a low dose sevoflurane, beta/low gamma (12-40...
Many general anesthetics potentiate gamma-aminobutyric acid (GABA) A receptors but their neuroanatomic sites of action are less clear. GABAergic neurons in the rostromedial tegmental nucleus (RMTg) send inhibitory projections to multiple arousal-promoting nuclei, role these modulating consciousness is unknown. In this study, designer exclusively activated by drugs (DREADDs) were targeted RMTg Vgat-ires-Cre mice. DREADDs expression was found and other brainstem regions. Activation decreased...
Abstract Scientific progress depends on reliable and reproducible results. Progress can also be accelerated when data are shared re-analyzed to address new questions. Current approaches storing analyzing neural typically involve bespoke formats software that make replication, as well the subsequent reuse of data, difficult if not impossible. To these challenges, we created Spyglass , an open-source framework enables analyses sharing both intermediate final results within across labs. uses...
New recording technologies and the potential for closed-loop experiments have led to an increasing demand computationally efficient accurate algorithms decode population spiking activity in multi-dimensional spaces. Exact point process filters can accurately low-dimensional signals, but are intractable high-dimensional signals. Approximate Gaussian efficient, inaccurate when signals complex distributions nonlinear dynamics. Even particle filter methods tend become inefficient distribution...
The brain has the remarkable ability to learn and guide performance of complex tasks. Decades lesion studies suggest that different regions perform specialized functions in support behaviors1-3. Yet recent large-scale neural activity reveal similar patterns encoding distributed widely throughout brain4-6. How these are compatible with regional specialization function remains unclear. Two frontal regions, dorsal medial prefrontal cortex (dmPFC) orbitofrontal (OFC), a paradigm this conundrum....
The cognitive ability to go beyond the present consider alternative possibilities, including potential futures and counterfactual pasts, can support adaptive decision making. Complex changing real-world environments, however, have many possible alternatives. Whether how brain select among them represent alternatives that meet current needs remains unknown. We therefore examined neural representations of spatial locations in rat hippocampus during navigation a complex patch foraging...
The emergence of deep learning techniques has provided new tools for the analysis complex data in field neuroscience. In parallel, advanced statistical approaches like point-process modeling provide powerful analyzing spiking activity neural populations. How and machine compare when applied to remains largely unclear. this research, we performance a filter long short-term memory (LSTM) network decoding 2D movement trajectory rat using recorded from an ensemble hippocampal place cells. We...
Summary paragraph The hippocampus is a vertebrate brain structure that expresses spatial representations 1 and critical for navigation 2,3 . Navigation in turn intricately depends on locomotion; however, current accounts suggest dissociation between hippocampal the details of locomotor processes. Specifically, thought to primarily represent higher-order cognitive variables like position, speed, direction movement 4–7 , while limb movements propel animal are be computed represented...