A5061702097- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Insect Utilization and Effects
- Insect Pheromone Research and Control
- Insect and Pesticide Research
- Invertebrate Immune Response Mechanisms
- Plant and animal studies
- Olfactory and Sensory Function Studies
- Animal Behavior and Reproduction
- Insect symbiosis and bacterial influences
- Physiological and biochemical adaptations
Johns Hopkins University
2019-2025
Brandeis University
2023
Temasek Life Sciences Laboratory
2010-2016
National University of Singapore
2010-2016
Gustatory pheromones play an essential role in shaping the behavior of many organisms. However, little is known about processing taste higher order brain centers. Here, we describe a male-specific gustatory circuit Drosophila that underlies detection anti-aphrodisiac pheromone (3R,11Z,19Z)-3-acetoxy-11,19-octacosadien-1-ol (CH503). Using behavioral analysis, genetic manipulation, and live calcium imaging, show Gr68a-expressing neurons on forelegs male flies exhibit sexually dimorphic...
Significance How sexual features and preferences originate evolve is one of the most important contentious problems in evolutionary biology. In some species, females choose male traits that indicate genetic quality or confer benefits. Thus, female are assumed to at same time coevolve. contrast, sensory exploitation occurs when males take advantage females’ preexisting biases. We show Drosophila by other through use a pheromone gain advantage. Notably, leads adaptation. These findings provide...
The yellow fever mosquito Aedes aegypti is a prolific vector of arboviral and filarial diseases that largely relies on its sense smell to find humans. To facilitate in-depth analysis the neural circuitry underlying Ae. olfactory-driven behaviors, we generated an updated in vitro atlas for antennal lobe olfactory brain region this disease using two independent neuronal staining methods. We performed morphological reconstructions with replicate fixed, dissected stained samples from adult male...
Abstract The mosquito Aedes aegypti is an emerging model insect for invertebrate neurobiology. We detail the application of a dual transgenesis marker system that reports nature transgene integration with circular donor template CRISPR–Cas9-mediated homology-directed repair at target chemoreceptor genes. Employing this approach, we demonstrate establishment cell-type-specific T2A-QF2 driver lines A. olfactory co-receptor genes Ir8a and orco via canonical CO2 receptor complex gene Gr1...
Abstract The yellow fever mosquito Aedes aegypti employs olfaction to locate humans. We applied neural activity mapping define the molecular and cellular logic of how brain is wired detect two human odorants that are attractive when blended together. determined breath volatile carbon dioxide (CO 2 ) detected by largest unit olfactory coding in antennal lobe brain. Synergistically, CO detection gates pre-synaptic calcium signaling sensory neuron axon terminals innervate unique regions tuned...
Eight analogues of (3R,11Z,19Z)-CH503 (3-acetoxy-11,19-octacosadien-1-ol), the anti-aphrodisiac pheromone male Drosophila melanogaster, were synthesized for a bioassay. These enantiomers 3-acetoxy-11,19-octacosadiyn-1-ol (1), 3-acetoxyoctacosan-1-ol (2), (Z)-3-acetoxy-11-octacosen-1-ol (3), and (Z)-3-acetoxy-19-octacosen-1-ol (4). None them pheromonally active, indicating that two double bonds at C-11 C-19 necessary bioactivity.
Unlike mammals, insects such as Drosophila have multiple taste organs. The chemosensory neurons on the legs, proboscis, wings and ovipositor of express gustatory receptors(1,2), ion channels(3-6), ionotropic receptors(7) that are involved in detection volatile non-volatile sensory cues. These directly contact tastants food, noxious substances pheromones therefore influence many complex behaviors feeding, egg-laying mating. Electrode recordings calcium imaging been widely used to quantify...
Abstract The yellow fever mosquito Aedes aegypti is a prolific vector of arboviral and filarial diseases that largely relies on its sense smell to find humans. To facilitate in-depth analysis the neural circuitry underlying Ae. olfactory-driven behaviors, we generated an updated in vitro atlas for antennal lobe olfactory brain region this disease using two independent neuronal staining methods. We performed morphological reconstructions with replicate fixed, dissected stained samples from...
Unlike mammals, insects such as Drosophila have multiple taste organs. The chemosensory neurons on the legs, proboscis, wings and ovipositor of express gustatory receptors1,2, ion channels3-6, ionotropic receptors7 that are involved in detection volatile non-volatile sensory cues. These directly contact tastants food, noxious substances pheromones therefore influence many complex behaviors feeding, egg-laying mating. Electrode recordings calcium imaging been widely used to quantify neuronal...