A5053148069- Biochemical and Molecular Research
- Enzyme Structure and Function
- Amino Acid Enzymes and Metabolism
- Bacterial Genetics and Biotechnology
- Microbial Natural Products and Biosynthesis
- Microbial Community Ecology and Physiology
- Prion Diseases and Protein Misfolding
- Pharmaceutical and Antibiotic Environmental Impacts
- Obstructive Sleep Apnea Research
- Tracheal and airway disorders
- Antibiotic Resistance in Bacteria
- Plant biochemistry and biosynthesis
- Bioactive Compounds and Antitumor Agents
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Neuroscience of respiration and sleep
- Microbial Metabolism and Applications
- Methane Hydrates and Related Phenomena
St Vincent's Hospital
2022
Box Hill Institute
2022
Florey Institute of Neuroscience and Mental Health
2022
The University of Melbourne
2021
CSIRO Land and Water
2016-2020
Monash University
2017-2020
Australian National University
2016-2020
Commonwealth Scientific and Industrial Research Organisation
2016-2017
ACT Government
2016
University of Otago
2016
Abstract Introduction Many patients with cognitive and neuropsychiatric symptoms face diagnostic delay misdiagnosis. We investigated whether cerebrospinal fluid (CSF) neurofilament light (NfL) total‐tau (t‐tau) could assist in the clinical scenario of differentiating neurodegenerative (ND) from psychiatric disorders (PSY), rapidly progressive disorders. Methods Biomarkers were examined specialist services (ND PSY) a national Creutzfeldt‐Jakob registry (Creutzfeldt‐Jakob disease [CJD]...
Abstract Cofactor F 420 plays critical roles in primary and secondary metabolism a range of bacteria archaea as low-potential hydride transfer agent. It mediates variety important redox transformations involved bacterial persistence, antibiotic biosynthesis, pro-drug activation methanogenesis. However, the biosynthetic pathway for has not been fully elucidated: neither enzyme that generates putative intermediate 2-phospho- l -lactate, nor function FMN-binding C-terminal domain γ-glutamyl...
A defining feature of mycobacterial redox metabolism is the use an unusual deazaflavin cofactor, F420 This cofactor enhances persistence environmental and pathogenic mycobacteria, including after antimicrobial treatment, although molecular basis for this remains to be understood. In work, we explored our hypothesis that by serving as a in antimicrobial-detoxifying enzymes. To test this, performed series phenotypic, biochemical, analytical chemistry studies relation model soil bacterium...
An unusual aspect of actinobacterial metabolism is the use redox cofactor F420. Studies have shown that F420H2-dependent reductases promiscuously hydrogenate diverse organic compounds in biodegradative and biosynthetic processes. These enzymes therefore represent promising candidates for next-generation industrial biocatalysts. In this work, we undertook first broad survey these as potential biocatalysts by exploring extent, well mechanistic structural bases, their substrate promiscuity. We...
SdhE is required for the flavinylation and activation of succinate dehydrogenase fumarate reductase (FRD). In addition, conserved in proteobacteria (α, β γ) eukaryotes. Although function this recently characterized family proteins has been determined, almost nothing known about how their genes are regulated. Here, RsmA (CsrA) RsmC (HexY) post-transcriptional post-translational regulators have identified shown to repress sdhEygfX expression Serratia sp. ATCC 39006. Conversely, flagella master...
F420 is a microbial cofactor that mediates wide range of physiologically important and industrially relevant redox reactions, including in methanogenesis tetracycline biosynthesis. This deazaflavin comprises redox-active isoalloxazine headgroup conjugated to lactyloligoglutamyl tail. Here we studied the catalytic significance oligoglutamate chain, which differs length between bacteria archaea. We purified short-chain (two glutamates) from methanogen isolate long-chain (five eight recombinant...
Mycobacteria are major environmental microorganisms and cause many significant diseases, including tuberculosis. make an unusual vitamin-like compound, F 420 , use it to both persist during stress resist antibiotic treatment. Understanding how mycobacteria is important, as this process can be targeted create new drugs combat infections like In study, we show that in a way different from other bacteria. We studied the molecular machinery determining chemical mechanism for identifying novel...
Abstract F 420 is a low-potential redox cofactor used by diverse bacteria and archaea. In mycobacteria, this has multiple roles, including adaptation to stress, cell wall biosynthesis, activation of the clinical antitubercular prodrugs pretomanid delamanid. A recent biochemical study proposed revised biosynthesis pathway for in mycobacteria; it was suggested that phosphoenolpyruvate served as metabolic precursor pathway, rather than 2-phospholactate long proposed, but these findings were...
Swan Hill District Health (SHDH) provides Otolaryngological services to outer regional Victoria. A preoperative checklist determines eligibility for tonsillectomy, and the role of investigations such as oximetry. Visiting specialists who provide T&A also remain on-site 24 h post-surgery. Management post-discharge complications is supported by SHDH's Emergency Department. Unstable patients are transferred tertiary care hospitals. This study examines safety outcomes associated with rural...
Abstract Cofactor F 420 plays critical roles in primary and secondary metabolism a range of bacteria archaea as low-potential hydride transfer agent. It mediates variety important redox transformations involved bacterial persistence, antibiotic biosynthesis, pro-drug activation methanogenesis. However, the biosynthetic pathway for has not been fully eluci-dated: neither enzyme that generates putative intermediate 2-phospho- L -lactate, nor function FMN-binding C-terminal domain γ-glutamyl...