A5052541350- Pediatric Hepatobiliary Diseases and Treatments
- Genetic and Kidney Cyst Diseases
- Pancreatic function and diabetes
- Liver physiology and pathology
- Pancreatic and Hepatic Oncology Research
- Organ Transplantation Techniques and Outcomes
- Birth, Development, and Health
- Genetics and Neurodevelopmental Disorders
- Liver Disease Diagnosis and Treatment
Cleveland Clinic Lerner College of Medicine
2017-2024
Cleveland Clinic
2017
The intrahepatic biliary network is a highly branched three-dimensional lined by epithelial cells, but how its branching patterns are precisely established not clear. We designed new computer-based algorithm that quantitatively computes the structural differences of networks. Utilizing algorithm, we showed inhibition Cyclin-dependent kinase 5 (Cdk5) led to reduced in zebrafish. Further, identified previously unappreciated downstream cascade regulated Cdk5. Pharmacological manipulations this...
Impaired formation of the intrahepatic biliary network leads to cholestatic liver diseases, which are frequently associated with autoimmune disorders. Using a chemical mutagenesis strategy in zebrafish combined computational analysis, we screened for novel genes involved formation. We positionally cloned mutation nckap1l gene, encodes cytoplasmic adaptor protein WAVE regulatory complex. The is located last exon after stop codon primary splice isoform, only disrupting previously unannotated...
Impaired formation of the biliary network can lead to congenital cholestatic liver diseases; however, genes responsible for proper system and maintenance have not been fully identified. Combining computational structure analysis algorithms with a zebrafish forward genetic screen, we identified 24 new mutants that display impaired intrahepatic formation. Complementation tests suggested these mutations affect different genes. We applied unsupervised clustering unbiasedly classify recovered...
The intrahepatic biliary network is a highly branched three-dimensional lined by epithelial cells, but how its branching patterns are precisely established not clear. We designed new computer-based algorithm that quantitatively computes the structural differences of networks. Utilizing algorithm, we showed inhibition Cyclin-dependent kinase 5 (Cdk5) led to reduced in zebrafish. Further, identified previously unappreciated downstream cascade regulated Cdk5. Pharmacological manipulations this...
Abstract Impaired formation of the biliary network can lead to congenital cholestatic liver diseases; however, genes responsible for proper system and maintenance have not been fully identified. Combining computational structure analysis algorithms with a zebrafish forward genetic screen, we identified 24 new mutants that display impaired intrahepatic formation. Complementation tests suggested these affect different genes. We applied unsupervised clustering classify recovered into three...