A5036694342- Retinal Development and Disorders
- Zebrafish Biomedical Research Applications
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Developmental Biology and Gene Regulation
- Wnt/β-catenin signaling in development and cancer
- Advanced Fluorescence Microscopy Techniques
- Neurogenesis and neuroplasticity mechanisms
- Cell Image Analysis Techniques
- Visual perception and processing mechanisms
- Photochromic and Fluorescence Chemistry
- Ocular Disorders and Treatments
- Neuroscience and Neural Engineering
- Neurobiology and Insect Physiology Research
- Circadian rhythm and melatonin
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Axon Guidance and Neuronal Signaling
- Spaceflight effects on biology
The Graduate University for Advanced Studies, SOKENDAI
2021-2022
Max Planck Institute for Biological Intelligence
2022
National Institute of Genetics
2018-2021
Max Planck Institute of Neurobiology
2014-2020
RIKEN Advanced Science Institute
2009-2010
Kyoto University
2003-2008
RIKEN Center for Emergent Matter Science
2008
Abstract Dense reconstruction of synaptic connectivity requires high-resolution electron microscopy images entire brains and tools to efficiently trace neuronal wires across the volume. To generate such a resource, we sectioned imaged larval zebrafish brain by serial block-face at voxel size 14 × 25 nm 3 . We segmented resulting dataset with flood-filling network algorithm, automated detection chemical synapses validated results comparisons transmission microscopic light-microscopic...
The ciliary marginal zone of the vertebrate retina contains undifferentiated progenitor cells that continue to proliferate and add new neurons glia peripherally during embryonic stages - even after formation a functional retina. To understand molecular mechanism controls prolonged cell proliferation in zone, we employed candidate molecule approach, focusing on Wnt2b (formerly know as Wnt13), which is expressed most tip Frizzled 4 5, seven-pass transmembrane Wnt receptors, were peripheral...
During the development of central nervous system, cell proliferation and differentiation are precisely regulated. In vertebrate eye, progenitor cells located in marginal-most region neural retina continue to proliferate for a much longer period compared ones retina, thus showing stem-cell-like properties. Wnt2b is expressed anterior rim optic vesicles, has been shown control marginal retina. this paper, we show that stable overexpression retinal explants inhibited cellular induced continuous...
Abstract Genetic access to small, reproducible sets of neurons is key an understanding the functional wiring brain. Here we report generation a new Gal4- and Cre-driver resource for zebrafish neurobiology. Candidate genes, including cell type-specific transcription factors, neurotransmitter-synthesizing enzymes neuropeptides, were selected according their expression patterns in small unique subsets from diverse brain regions. BAC recombineering, followed by Tol2 transgenesis, was used...
Optogenetic actuators with diverse spectral tuning, ion selectivity and kinetics are constantly being engineered providing powerful tools for controlling neural activity subcellular resolution millisecond precision. Achieving reliable interpretable in vivo optogenetic manipulations requires reproducible actuator expression calibration of photocurrents target neurons. Here, we developed nine transgenic zebrafish lines stable opsin calibrated their efficacy vivo. We first used high-throughput...
In the vertebrate retina, stem cell-like progenitor cells are maintained in a distinct region called ciliary marginal zone (CMZ). Canonical Wnt signaling regulates maintenance of CMZ. However, its downstream molecular mechanisms have remained largely unclear. Here, we show that chick Hairy1, an established Notch effector, mediates Wnt-dependent CMZ chicken. Interestingly, unlike other developmental contexts which Hes gene expression is regulated by signaling, Hairy1 signaling. necessary and...
Purpose.: Development of the retinal pigment epithelium (RPE) is controlled by intrinsic and extrinsic regulators including orthodenticle homeobox 2 (Otx2) Wnt/β-catenin pathway, respectively. Otx2 β-catenin are necessary for expression RPE key regulator microphthalmia-associated transcription factor (Mitf); however, neither sufficient to promote Mitf in vivo. The study was conducted determine whether act a combinatorial manner tested co-expression presumptive chick retina induces ectopic...
Understanding brain-wide neuronal dynamics requires a detailed map of the underlying circuit architecture. We built an interactive cellular-resolution atlas zebrafish brain at six days post-fertilization (6 dpf) based on reconstructions over 2,000 individually GFP-labeled neurons. clustered our dataset in 'cell types', establishing unique database quantitatively described morphologies together with their spatial coordinates vivo. Over hundred transgene expression patterns (mainly Gal4 lines)...
Spontaneous activity during the early postnatal period is thought to be crucial for establishment of mature neural circuits. It remains unclear if peripheral structure developing somatosensory system exhibits spontaneous activity, similar that observed in retina and cochlea mammals. By establishing an ex vivo calcium imaging system, here we found neurons whisker-innervating region trigeminal ganglion (TG) neonatal mice generate activity. A small percentage showed some obvious correlated...
Abstract Basic helix–loop–helix (bHLH) transcription factors play important roles in cell type specification and differentiation during the development of nervous system. In this study, we identified a chicken homolog Atonal 8/ath6 (Cath6) examined its role developing retina. Unlike other Atonal‐family proneural genes that induce neuronal differentiation, Cath6 was expressed stem cell‐like progenitor cells marginal region retina, overexpression inhibited differentiation. A fused with VP16...
Abstract Optogenetic actuators with diverse spectral tuning, ion selectivity and kinetics are constantly being engineered providing powerful tools for controlling neural activity subcellular resolution millisecond precision. Achieving reliable interpretable in vivo optogenetic manipulations requires reproducible actuator expression calibration of photocurrents target neurons. Here, we developed nine transgenic zebrafish lines stable opsin calibrated their efficacy . We first used...