A5088901287- Microtubule and mitosis dynamics
- Malaria Research and Control
- Respiratory viral infections research
- Ubiquitin and proteasome pathways
- Cancer therapeutics and mechanisms
- Protein Degradation and Inhibitors
- Berberine and alkaloids research
- Histone Deacetylase Inhibitors Research
- Complement system in diseases
- Cellular transport and secretion
- Glycosylation and Glycoproteins Research
- Cancer-related Molecular Pathways
- Drug Transport and Resistance Mechanisms
- Redox biology and oxidative stress
- Toxin Mechanisms and Immunotoxins
- Quinazolinone synthesis and applications
- S100 Proteins and Annexins
- Genomics and Chromatin Dynamics
- Peptidase Inhibition and Analysis
- Click Chemistry and Applications
- Retinal Development and Disorders
- PARP inhibition in cancer therapy
- Synthesis and bioactivity of alkaloids
- 14-3-3 protein interactions
- Influenza Virus Research Studies
Virologie et Immunologie Moléculaires
2021-2024
Université Paris-Saclay
2021-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2024
Florida Atlantic University
2015-2019
Université de Montpellier
2010-2017
Institut des Biomolécules Max Mousseron
2017
Inserm
2016
Aix-Marseille Université
2016
National Academy of Sciences of Ukraine
2013-2015
Centre National de la Recherche Scientifique
2004-2013
Relocation, relocation: Inactivation of the mitotic kinesin MKLP-2 by a specific small-molecule inhibitor (1) leads to failure in recruitment chromosome passenger protein (survivin, red) central spindle (microtubules, green) during anaphase segregation (DNA, blue). Detailed facts importance specialist readers are published as ”Supporting Information”. Such documents peer-reviewed, but not copy-edited or typeset. They made available submitted authors. Please note: The publisher is responsible...
Respiratory syncytial virus (RSV) can cause pulmonary complications in infants, elderly and immunocompromised patients. While two vaccines prophylactic monoclonal antibodies are now available, treatment options still needed. JNJ-7184 is a non-nucleoside inhibitor of the RSV-Large (L) polymerase, displaying potent inhibition both RSV-A -B strains. Resistance selection hydrogen-deuterium exchange experiments suggested binds RSV-L connector domain. prevented RSV replication transcription by...
Background. The pathogenesis of influenza A virus (IAV) infections is a multifactorial process that includes the replication capacity and harmful inflammatory response to infection. Formyl peptide receptor 2 (FPR2) emerges as central in processes controlling resolution acute inflammation. Its role has not been investigated yet. Methods. We used pharmacologic approaches investigate FPR2 during IAV infection vitro vivo. Results. In vitro, expressed on A549 cells was activated by IAV, which...
PfEMP1 is a family of adhesive proteins expressed on the surface Plasmodium falciparum-infected erythrocytes (IEs), where they mediate adhesion IEs to range host receptors. Efficient PfEMP1-dependent IE sequestration often depends soluble serum proteins, including IgM. Here, we report comprehensive investigation which about 60 var gene-encoded variants per parasite genome can bind IgM via Fc part antibody molecule, and constituent domains those are involved. We erased epigenetic memory gene...
Respiratory syncytial virus (RSV) is a negative sense single-stranded RNA and one of the main causes severe lower respiratory tract infections in infants young children. RSV replication/transcription capping are ensured by viral Large (L) protein. The L protein contains polymerase domain associated with polyribonucleotidyl transferase its N-terminus, methyltransferase (MTase) followed C-terminal (CTD) enriched basic amino acids at C-terminus. MTase-CTD Mononegavirales forms clamp to...
Major complications and mortality from Plasmodium falciparum malaria are associated with cytoadhesion of parasite-infected erythrocytes (IE). The main parasite ligands for members the P. erythrocyte membrane protein 1 (PfEMP1) family. Interactions different host receptor-ligand pairs may lead to various pathological outcomes, like placental or cerebral malaria. It has been shown previously that IE can bind integrin αVβ3. Using bead-immobilized PfEMP1 constructs, we have identified PFL2665c...
Cyclin-dependent kinases constitute attractive pharmacological targets for cancer therapeutics, yet inhibitors in clinical trials target the ATP-binding pocket of CDK and therefore suffer from limited selectivity emergence resistance. The more recent development allosteric targeting conformational plasticity protein offers promising perspectives therapeutics. In particular tampering with T-loop dynamics CDK2 kinase would provide a selective means inhibiting this kinase, by preventing its...
The Rab small G-protein family plays important roles in eukaryotes as regulators of vesicle traffic. In proteins, the hydrolysis GTP to GDP is coupled with association and dissociation from membranes. Conformational changes related their different nucleotide states determine effector specificity. crystal structure human neuronal Rab6B was solved its `inactive' (with bound MgGDP) `active' (MgGTPγS-bound) forms 2.3 1.8 Å, respectively. Both crystallized space group P212121, similar unit-cell...
Amitozyn (Am) is a semi-synthetic drug produced by the alkylation of major celandine (Chelidonium majus L.) alkaloids with organophosphorous compound N,N’N’-triethylenethiophosphoramide (ThioTEPA). We show here that treatment living cells Am reversibly perturbs microtubule cytoskeleton, provoking dose-dependent cell arrest in M phase. changed dynamics tubulin polymerization vitro, promoted appearance aberrant mitotic phenotypes HeLa and induced apoptosis activation caspase-9, caspase-3 PARP,...
Malaria caused by Plasmodium falciparum is the most virulent form of malaria, leading to approximately a half million deaths per year. Chemotherapy continues be key approach in malaria prevention and treatment. Due widespread parasite drug resistance, identification development new anti-malarial compounds remains an important task malarial parasitology. The semi-synthetic amitozyn, obtained through alkylation major celandine (Chelidonium majus) alkaloids with...
The use of cytotoxic agents to eliminate cancer cells is limited because their nonselective toxicity and unwanted side effects. One the strategies overcome these limitations latent prodrugs that become toxic in situ after being enzymatically activated target cells. In this work we describe a method for producing tumor-specific toxins by using ubiquitin fusion technique. illustrated production recombinant in-frame saporin, toxin from plant Saponaria officinalis. Ubiquitin-fused were rapidly...
Amitozyn (Am) is a semi-synthetic drug produced by the alkylation of major celandine (Chelidonium majus L.) alkaloids with organophosphorous compound N,N'N'-triethylenethiophosphoramide (ThioTEPA).We show here that treatment living cells Am reversibly perturbs microtubule cytoskeleton, provoking dose-dependent cell arrest in M phase.Am changed dynamics tubulin polymerization vitro, promoted appearance aberrant mitotic phenotypes HeLa and induced apoptosis activation caspase-9, caspase-3...
Human respiratory syncytial virus (hRSV) is a major cause of illness in young children and can severe infections the elderly or immunocompromised adults. To date, there no vaccine to prevent hRSV infections, disease management limited preventive care by palivizumab infants supportive for Intervention with small-molecule antivirals specific represents good alternative, but such compounds are currently approved. The investigation existing drugs new therapeutic purposes (drug repositioning) be...
Abgedrängt: Die Inaktivierung des mitotischen Kinesins MKLP-2 durch den spezifischen niedermolekularen Inhibitor 1 verhindert, dass das Protein Survivin (rot im Bild; DNA blau, Mikrotubuli grün) während der Chromosomentrennung in Anaphase zur Spindel gelangt.